Eubacterium ventriosum and two-shape eubacterium preparation and application thereof

A technology of Eubacterium phleiformis and Eubacterium tumefaciens, applied in the field of biopharmaceuticals, can solve the problem that the curative effect is not very ideal, etc.

Inactive Publication Date: 2009-12-02
QINGDAO EASTSEA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main ingredients of the current microecological preparations include Bifidobacterium, Lactobacillus, Enterococcus, B

Method used

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  • Eubacterium ventriosum and two-shape eubacterium preparation and application thereof
  • Eubacterium ventriosum and two-shape eubacterium preparation and application thereof
  • Eubacterium ventriosum and two-shape eubacterium preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0038] Preparation of Medicine Example 1 Preparation of Oral Protruding Eubacterium Live Bacteria Powder, Oral Dimorph Bacteria Live Bacteria Powder and Dual Live Bacteria Powder

[0039] 1 Preparation of bacteria powder and identification of strains

[0040] The feces of a healthy baby in Jiaonan City, Shandong Province were collected, and then the feces were placed in a sterilized anaerobic bottle, and nitrogen gas was blown in while fully mixing, and 2 grams of feces were quickly added to 18 mL of sterilized diluent, and nitrogen gas was blown in while fully mixing. Evenly, in the aseptic operating table, carry out 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Gradient dilution, take 10 -5 、10 -6 、10 -7 Three dilution gradients, spread on the solid medium for the selective single colony isolation of Eubacterium protrudoides or Eubacterium dimorphus, place in an anaerobic tank, culture at 37°C for 48 hours in anaerobic conditions, and select two single colonies wi...

Embodiment 1

[0055] Example 1. Research on the repairing effect of Eubacterium protrudoides and Eubacterium dimorphus on intestinal mucosa

[0056] 1 Materials and methods

[0057] 1.1 Materials

[0058] 1.1.1 Drugs Protruding fungus bacterial liquid (10 7 CFU / mL); Amphimorpha bacterial liquid (10 7 CFU / mL); dual live bacteria solution (10 7 CFU / mL); Bifidobacteria liquid (10 7 CFU / mL); dextran sodium sulfate (DSS, 10g / bottle, Sino-American Biotec); Alcian blue (1g / bottle, Sino-American Biotec).

[0059] 1.1.2 Animals 70 SD male rats, weighing 80-100 g, standard grade 2, were purchased from the Experimental Animal Center of China Institute for the Control of Pharmaceutical and Biological Products.

[0060] 1.2 Method

[0061] 1.2.1 Modeling and treatment of intestinal mucosal injury

[0062] There were 70 rats, 10 rats were used as the normal control group, and the other 60 rats were fed with 3% DSS solution (1mL / 100g) to the fasting rats, once a day for 7 days, and the last time th...

Embodiment 2

[0071] Example 2. Studies on the Regulatory Expression of Immunity and Inflammatory Factors by Eubacterium protrudoides and Eubacterium amphimorpha

[0072] 1 Experimental materials and methods

[0073] 1.1 Experimental animals: male SD rats, 180-220g.

[0074] 1.2 Experimental drug: mesalamine (200mg / mL); protruding fungus liquid (10 7 CFU / mL); Amphimorpha bacterial liquid (10 7 CFU / mL); dual live bacteria solution (10 7 CFU / mL); Bifidobacteria liquid (10 7 CFU / mL). Provided by Qingdao Donghai Pharmaceutical Co., Ltd.

[0075] 1.3 Experimental reagents: Freund's complete adjuvant (sigma), MTT (sigma); concanavalin (ConA) (Sigma); lipopolysaccharide (LPS) (Sigma); MTT (Sigma), purchased from Beijing Shubowei Chemical Industry Instrument Co., Ltd.; 1640 culture medium (Gibco); lyophilized powder of bovine colonic mucosal protein (self-made); IL-8 ELISA kit (BD); TNF-α ELISA kit (Ebioscience); rat IgG reference serum; Mouse IgG antiserum was purchased.

[0076] 2 Experim...

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Abstract

The invention relates to an eubacterium ventriosum and two-shape eubacterium preparation and application thereof, in particular to microbial ecological preparation prepared by using eubacterium ventriosum or/and two-shape eubacterium as main active ingredients, and application of the microbial ecological preparation for treating relative diseases. The microbial ecological preparation comprises medicine composition, health products, drinks, veterinary drug and feed additive.

Description

technical field [0001] The present invention relates to preparations of Eubacterium trachees and Eubacterium dimorphus and their application, in particular to the preparation of Eubacterium trachees and Eubacteria dimorphus alone or in combination as the main active ingredients to make a microecological preparation, and the use of the microecological preparation in the treatment of related diseases The application in the invention belongs to the field of biopharmaceuticals. Background technique [0002] At present, the harm of the misuse of antibacterial drugs in clinical and aquaculture has attracted attention. Since probiotics have no side effects, the use of probiotics to treat related diseases, health care, and as veterinary drugs and feed additives has been widely used. The main ingredients of current probiotics include Bifidobacterium, Lactobacillus, Enterococcus, Bacillus licheniformis, Bacillus subtilis, etc., and the curative effect of treating related diseases is n...

Claims

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Application Information

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IPC IPC(8): A61K35/74A23L1/30A23K1/16A61P1/00A61P1/02A61P1/04A61P1/10A61P1/12A61P1/14A61P3/02A61P3/06A61P9/00A61P9/10A61P9/12A61P11/00A61P17/00A61P19/02A61P19/08A61P29/00C12N1/20
Inventor 崔云龙李洪福王发合
Owner QINGDAO EASTSEA PHARMA
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