Gene chip for parallelly detecting various upper respiratory track viruses

Abstract

The invention relates to gene chips for parallelly detecting various upper respiratory track viruses, in particular to gene chips arranged in a matrix form by using probes of nucleotide sequences complementing to respiratory track viruses DNA / RNA, which combines with polymerase chain reaction (PCR) technology and is used for rapidly and parallelly detecting and distinguishing more than 20 common respiratory track viruses. The gene chips can take a nylon membrane, a silicon chip, a glass sheet or a plastic sheet as a substrate, and contain a specific probe array (comprising 1, the gene chip; 2, a probe region; 3, a positioning point; 4, a positive quality control point; 5, a negative charging point; and specific probes) aiming at genotyping. The invention fully combines the advantage that the gene chips can simultaneously detects a plurality of genes in a sample at a time and the advantage of high specificity and sensitivity of the polymerase chain reaction (PCR) technology, and has broad coverage of parallel detection. The gene chips have the advantages of simple, convenient and fast operation, high specificity, high sensitivity, intuitional judgment of results and the like.

Description

technical field [0001] The present invention relates to a kind of gene chip that detects multiple respiratory viruses in parallel, in particular to a gene chip that utilizes probes of nucleotide sequences complementary to respiratory virus DNA / RNA to arrange in matrix form and multiplex polymerase chain reaction (PCR) The technology is used for rapid parallel detection and resolution of various respiratory viruses, and belongs to the technical field of gene chips and diagnostic reagents. Background technique [0002] Acute respiratory infection is a common disease in the world, frequently-occurring disease, its main cause is caused by various respiratory viruses, the common ones are influenza A virus (including H1, H3, H5 subtypes), influenza B virus, para Influenza virus (including A, B, C, D), respiratory fusion virus (including A, B), rhinovirus, enterovirus, OC43 coronavirus, SARS coronavirus, 229E Type coronavirus, human metapneumovirus, Mycoplasma pneumoniae, Chlamydi...

AI Technical Summary

Problems solved by technology

Due to the many types of possible pathogens, the isolation and culture of viruses is not only long-term and costly, but also technically difficult

It is also difficult to quickly identify pathogens with immunological detection methods

However, the common limitation of the current popular methods is that they can only detect a single virus at a time, and the sensitivity of the detection method is not high enough

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