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Method for extracting superoxide dismutase

A superoxide and dismutase technology, applied in the direction of oxidoreductase, etc., can solve the problems that the specific activity and purity of enzymes are difficult to achieve a comprehensive balance, superoxide dismutase is limited, and it is difficult to meet market needs, etc., to achieve good resource development and utilization Foreground, easy artificial afforestation, strong natural regeneration effect

Inactive Publication Date: 2010-01-20
SICHUAN UNIV
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Problems solved by technology

With the frequent reports of malignant infectious diseases such as mad cow disease, avian influenza, and mouth-to-mouth disease all over the world, the risk of producing animal-derived blood products has increased, resulting in the extraction of superoxide dismutase due to limited raw materials, which has a low output and is difficult to meet market needs. question
Moreover, the existing method for extracting superoxide dismutase has complex process steps, and the specific activity and purity of the enzyme are difficult to achieve a comprehensive balance

Method used

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  • Method for extracting superoxide dismutase
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Examples

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Embodiment

[0039] The fresh jatropha seeds used in this example were collected from the botanical garden of the College of Life Sciences, Sichuan University; the reagents used included: Sephadex G2100 (product of Pharmacia), protein marker, bovine serum albumin, acetone, chloroform, ammonium sulfate, etc. were of domestic analytical grade. Nitroblue Tetrazolium Chloride (NBT), Methionine, Diethylaminoethylcellulose (DEAE), Tetramethylethylenediamine (TEMED), Tris (Tris), Propylene Amide (Sinopharm Chemical Reagent Co., Ltd.), methylene bisacrylamide (Shanghai Chemical Reagent Company, China Pharmaceutical Group) and other chemical reagents are all analytically pure, and the standard protein is bovine serum albumin produced by Shanghai Biochemical Reagent Company, Coomassie Brilliant Blue G-250 (Shanghai Chemical Reagent Packing Factory); the instruments used include: UV29100 ultraviolet-visible spectrophotometer (Beijing Ruili Analytical Instrument Factory), high-speed refrigerated centri...

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Abstract

The invention relates to a method for extracting superoxide dismutase, in particular to a method for extracting superoxide dismutase from barbadosnut. The method for extracting superoxide dismutase is achieved by using seeds or leaves of barbadosnut as raw materials, and comprises the steps of the preparing crude extract, removing foreign protein, sectional salting-out ammonium sulfate, precipitating acetone, carrying out Sephadex G2100 molecular sieve column chromatography, and the like. The invention provides a new source for extracting superoxide dismutase, and optimizes technical parameters for extracting superoxide dismutase. The method for extracting superoxide dismutase can give consideration to both the specific activity and the recovery ratio of enzyme, and is beneficial to the large-scale production of enterprises.

Description

technical field [0001] The invention relates to a method for extracting superoxide dismutase, in particular to a method for extracting superoxide dismutase from Jatropha curcas. Background technique [0002] Superoxide dismutase (SOD) is a metalloenzyme commonly found in animals, plants and microorganisms. According to the different metal ions bound to the active site, superoxide dismutase can be divided into four types: CuZn-SOD, Mn-SOD, Fe-SOD and Ni-SOD. Superoxide dismutase is an antioxidant enzyme that specifically removes superoxide anion radicals in organisms. It can catalyze the dismutation of superoxide anion radicals into molecular oxygen and hydrogen peroxide, thereby reducing the harm of oxygen free radicals to the body. [0003] Oxygen free radicals are related to most diseases and the aging of the body. The massive accumulation of free radicals can cause protein denaturation and cross-linking, causing many enzymes and hormones in the body to lose their biolog...

Claims

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Application Information

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IPC IPC(8): C12N9/02
Inventor 谢无畏陈放欧阳超唐琳徐莺王胜华
Owner SICHUAN UNIV
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