Hemokinin-iron oral liquid

A technology of heme peptide and iron oral liquid, which is applied in the direction of blood diseases, extracellular fluid diseases, peptide/protein components, etc., can solve problems such as bad taste, damage to the balance of metal ions in the human body, and adverse reactions, so as to avoid residual toxicity and odor, realize real-time automatic operation, and avoid the effect of losing active ingredients

Inactive Publication Date: 2010-03-10
张滨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Treat IDA, adopt iron therapy more at home and abroad, although these products have certain curative effect, there are following problems: (1) low bioavailability: just be difficult to be absorbed by human body as the ferrous sulfate that has been popular for more than 200 years, the absorption rate About 1.6%, other inorganic iron and organic iron, the absorption rate is also between 3% and 5%
(2) Large side effects: These iron products all destroy the balance of metal ions in the human body and cause adverse reactions in the gastrointestinal tract. In order to reduce the side effects of gastrointestinal discomfort, they are required to be taken after meals. Inhibits iron absorption, making its absorption rate even lower
(3) Poor taste, difficult to adhere to: These products often have a certain rusty taste, and the taste is not good. In addition, the treatment of IDA often requires sev...

Method used

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  • Hemokinin-iron oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Separation of red blood cells from livestock and poultry blood

[0043] (1) blood collection

[0044] Healthy livestock and poultry that have passed the inspection are fasted for 24 hours (only water is fed during the period), transported to the slaughtering workshop, and the blood is quickly collected in a sealable container (non-toxic plastic bag, non-toxic plastic bottle, stainless steel) with a tube-type vacuum blood sampling knife. and other containers).

[0045] (2) Anticoagulant treatment

[0046] 3-5 minutes before blood collection, use 0.2% trisodium citrate and 0.15% sodium citrate mixture as anticoagulant, add 5% of the blood collection volume into the blood collection container, and stir with the blood at 100r / min for 3 minutes .

[0047] (3) Separation of red blood cells

[0048] Add 10% of blood volume 2% D-glucose, 0.85% NaCl, 0.05% citric acid, 0.08% sodium citrate to anticoagulated blood to prepare cell dispersion liquid, let it stand for...

Embodiment 2

[0049] Example 2: Red blood cell wall breaking

[0050] (1) Red blood cell wall breaking

[0051] Add distilled water twice the amount of red blood cell sediment, and place it in an ultrasonic processor for 2 minutes to break the red blood cell wall and release hemoglobin.

[0052] (2) Adjust the pH of the hemoglobin solution

[0053] Adjust the pH value of the hemoglobin solution so that it conforms to the optimal pH value for protease degradation. If trypsin is used, the pH value should be adjusted to 8.0, and if neutral protease is used, the pH value should be adjusted to 7.0. The pH adjustment time should be completed within 2 minutes, mainly It is to prevent the oxidation reaction of iron ions in hemoglobin.

Embodiment 3

[0054] Embodiment 3: Under vacuum high-frequency pulse environmental conditions, protease degrades hemoglobin

[0055] (1) Requirements for equipment for vacuum high-frequency pulse proteolysis of hemoglobin

[0056] Vacuum chamber: The vacuum chamber can withstand the vacuum degree below 0.01MPa, and the vacuum degree change is within ±0.2Pa within 6 hours.

[0057] High-frequency pulse: the pulse electric field parameter is T 1 -500.0us, T 2 500.0us, Δ1.000ms, f1.000KHz, V 1 1.88v, V 2 -120mv, h20.3, the pulse wave type is two-way square wave, the electrode distance is 1.2cm, and the pulse frequency is 200kHz.

[0058] (2) Add protease

[0059] Add 6000 U / mL protease solution to the solution that has been broken and adjust the pH of the hemoglobin solution, stir at 50 r / min for 2 minutes, seal the container, and place it in a vacuum chamber.

[0060] (3) Insert the pulse electrode

[0061] Insert the pulse electrode connected to the pulse signal generator into the air...

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Abstract

The invention provides a divalent hemokinin-iron oral liquid, the preparation method thereof comprises: separating hematid out of blood of livestock or poultry, adding doubleness distilled water, combining ultrasonication to break wall, releasing hemoglobin, adjusting pH value of hemoglobin solution, adding protease, placing in a vacuum dry chamber, leading in pulse current for acting for 4 hours,carrying out heating enzyme killing treatment at 95 DEG C for 30 minutes, obtaining divalent hemokinin-iron with purity more than 90% by metal-chelating affinity chromatography and electromagnetic separation, then adding tomato juice, red date juice, liquorice juice, angelica sinensis juice, astragalus mongholicus juice, juice of dried rhizome of rehmannia, stevioside, glycoprotein, carrageenan and algae ester sodium to blend so as to obtain the hemokinin-iron oral liquid. The oral liquid beverage product prepared by the invention has small dose, good mouth feel and high portability, can be directly absorbed by intestinal mucosal cells, cannot directly generate any digestion characteristic irritation symptom, and the absorptivity is high.

Description

technical field [0001] The present invention relates to a biological product and its preparation method, in particular, it provides a preparation method of divalent heme peptide-iron and its separation and purification method. The fields of application are iron supplement capsules, iron supplement granules, iron supplement oral liquids or iron supplement beverages. Background technique [0002] Iron is an indispensable nutrient in the vast majority of organisms. It contains about 4-5 grams in the adult body, accounting for only 1 / 10,000 of the body weight. present in the form of ferritin (23%). But its role is very important. Iron is the basic raw material for the body to produce hemoglobin, myoglobin and iron-containing enzymes. It is an essential trace element for the human body. It participates in the transportation of oxygen and carbon dioxide in the body and is a component of tissue respiratory enzymes such as cytochrome oxidase and peroxidase. . Therefore, the impo...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61K9/00A61P3/02A61P7/06C12P21/06A23L2/00A61K36/81A61K36/815A61K35/64A61K35/18
Inventor 张滨
Owner 张滨
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