A method for continuous extraction and separation of 1-deoxynojirimycin (dnj) and flavonoids from mulberry leaves

Abstract

A method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavonoids from mulberry leaves, the main points of the method are: extracting after mixing mulberry leaves and ethanol, centrifuging and filtering, adding a flocculant to flocculate and precipitate after concentrating; removing the precipitate Finally, the filtrate is added to a plurality of connected cation exchange resin columns, and flows through the macroporous adsorption resin column connected in series with the resin column; after the filtrate is added, it is washed with pure water until the macroporous resin column effluent is colorless. It is disconnected, and then eluted 1-deoxynojirimycin (DNJ) from the cationic resin column with ammonia water or ammonia-containing ethanol solution; Concentrate and filter with a filter membrane, and then spray dry to obtain 1-deoxynojirimycin (DNJ) in the form of brown yellow powder. Wash the macroporous resin column with 3%-10% ethanol to remove colloidal impurities, then elute flavonoids with 50%-70% ethanol, collect the brown-red eluate, concentrate under reduced pressure to recover ethanol, and then spray dry to obtain yellow-brown Powdered total flavonoids of mulberry leaves.

Description

technical field [0001] The invention relates to a method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavonoids from mulberry leaves. Background technique [0002] Mulberry leaf is the dry leaf of Moraceae plant mulberry, which is cultivated or wild, and has strong adaptability to soil, and it is mostly produced in most parts of the country. Bitter in the mouth, sweet, cold in nature. Studies have found that mulberry leaves have anti-inflammatory and hypoglycemic effects pharmacologically, and can lower blood fat and diuresis. Mulberry leaves have the above activities and pharmacological effects, mainly because their components contain 1-deoxynojirimycin (DNJ), flavonoids and other components, so it is very necessary to extract them from mulberry leaves for concentrated use . [0003] 1-Deoxynojirimycin (DNJ) is a strong α-glucosidase inhibitor, which inhibits α-glucosidase in the small intestine to decompose disaccharides such as sucrose and m...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that a high-speed centrifuge is used for material-liquid separation, which is expensive in industry; in addition, the material liquid is separated from flavonoids through a macroporous resin, and then the alkaloids are extracted through a cationic resin, which is easy to lose precious and relatively low content. Low active ingredient 1-deoxynonomycin (DNJ) (0.2%-0.4% in mulberry leaves)

But its disadvantage is that this process uses organic solvents such as chloroform and n-hexane when preparing alkaloids, which are toxic and difficult to remove from the product, and are not suitable for large-scale industrial production

The advantage is that the separated product can be easily obtained, but the disadvantage is that the production step needs to cool the extract to 10°C to -30°C, the conditions are relatively harsh, and methanol, diethylamine, triethylamine are used in the process Toxic and flammable organic solvents, which are not conducive to clean production