Method for preparing lactobacillus microcapsules

A technology of lactic acid bacteria microcapsules and microcapsules is applied in the directions of microcapsule preparations, microsphere preparation, food forming, etc., and can solve the problems of low yield of lactic acid bacteria microcapsules, uneven dispersion of lactic acid bacteria, high mortality of lactic acid bacteria, and achieve food safety. High performance, avoiding uneven dispersion, and reducing serum cholesterol

Inactive Publication Date: 2010-03-31
广州万物生健康产业有限公司
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Problems solved by technology

[0007] The purpose of the present invention is to solve the difficulty in obtaining dry lactic acid bacteria microcapsule powder in the current preparation method of lactic acid bacteria microcapsules, uneven dispersion of lactic acid bacteria, high mortality rate of lactic acid bacteria in the preparation process, the crosslinking agent used has certain toxicity, and the yield of lactic acid bacteria microcapsules low defect, and a kind of preparation method of lactic acid bacteria microcapsule provided

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  • Method for preparing lactobacillus microcapsules
  • Method for preparing lactobacillus microcapsules
  • Method for preparing lactobacillus microcapsules

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specific Embodiment approach 1

[0015] Specific embodiment one: the lactic acid bacteria microcapsules of this embodiment are prepared according to the following steps: 1. Mix the microcapsule core material and the microcapsule wall material evenly according to the volume ratio of 1:2 to obtain a mixed solution, wherein the microcapsule core material is added Protective agent and prebiotic lactic acid bacteria liquid, the microcapsule wall material is a sodium alginate solution with an emulsifier added, the mass concentration of sodium alginate in the microcapsule wall material is 1.8% to 2.2%, and the emulsifier in the microcapsule wall material It is Tween-20, the mass concentration of emulsifier is 0.09%~0.11%; 2, the mixed liquid that step 1 obtains is added dropwise in the salad oil that contains stearic acid and emulsifier Span-80, and at 37 ℃, rotating speed Under the condition of 1080~1140r / min, it is emulsified for 14~16min to form a primary emulsion, wherein the volume ratio of the dripping amount o...

specific Embodiment approach 2

[0027] Specific embodiment two: the lactic acid bacteria microcapsules of this embodiment are prepared according to the following steps: 1. Mix the microcapsule core material and the microcapsule wall material evenly according to the volume ratio of 1:2 to obtain a mixed solution, wherein the microcapsule core material is added Protective agent and prebiotic lactic acid bacteria liquid, the microcapsule wall material is sodium alginate solution with emulsifier added, the mass concentration of sodium alginate in the microcapsule wall material is 2%, and the emulsifier in the microcapsule wall material is Tween- 20. The mass concentration of the emulsifier is 0.1%; 2. Add the mixed liquid obtained in step 1 dropwise to the salad oil containing stearic acid and emulsifier Span-80, and heat it under the conditions of 37°C and 1100r / min Lower emulsification for 15min forms primary emulsion, wherein the volume ratio of the amount of mixed solution added to the salad oil is 1:2, the m...

specific Embodiment approach 3

[0059] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the concentration of the lactic acid bacteria bacterial liquid in step one is 0.46×10 9 cfu / mL Lb.paracasei HD1.7 bacteria liquid. Other steps and parameters are the same as those in Embodiment 1 or 2.

[0060] In this embodiment, Lactobacillus paracasei HD1.7 (Lb.paracasei HD1.7) has been preserved in the China Type Culture Collection Center, the preservation number is CCTCC M205015, and it has a Chinese invention patent "peptide natural microbial preservative producing bacteria and Applications and preparation methods of preservatives" (publication number CN1670184A, published on September 21, 2005). Lactobacillus paracasei HD1.7 (Lb.paracasei HD1.7) was isolated in 2002 from the fermentation broth of L-lactic acid bacteria pickled sauerkraut by the Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University.

[0061] Lb.paracasei HD1.7 belo...

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Abstract

The invention discloses a method for preparing lactobacillus microcapsules, which relates to a method for preparing microcapsules. The method solves the defects that the prior method for preparing thelactobacillus microcapsules cannot obtain dry lactobacillus microcapsule powder easily, the lactobacilli are diffused unevenly, the mortality rate of the lactobacilli is high in the preparation process, a used crosslinking agent has certain toxicity, and the yield of the lactobacillus microcapsules is low. The method for preparing the lactobacillus microcapsules comprises the following steps: firstly, mixing a core material and a wall material; secondly, preparing a primary emulsion; thirdly, preparing a microcapsule oil solution; fourthly, preparing a multiple emulsion; and fifthly, performing condensation, solidification and vacuum freeze drying. The method for preparing the lactobacillus microcapsules can obtain the dry lactobacillus microcapsule powder and avoid the defects of the uneven diffusion of the lactobacilli and the high mortality rate by means of the vacuum freeze drying; the method uses no crosslinking agents and has no toxic action on the lactobacilli; the external water phase is not miscible with the internal water phase; and the yield of the lactobacillus microcapsules prepared by the method reaches 84.6 percent, and the freeze-drying survival rate reaches over 60.107 percent.

Description

technical field [0001] The invention relates to a preparation method of microcapsules. Background technique [0002] At present, the interface polymerization method, phase separation method, extrusion method, double emulsion method and spray drying method are mainly used to prepare lactic acid bacteria microcapsules. [0003] The phase separation method, also known as the coagulation method, is to emulsify or disperse the core material containing lactic acid bacteria in the continuous phase in which the wall material is dissolved, and then reduce the solubility of the wall material and separate it from the continuous phase to form a viscous liquid phase (not precipitation ), wrapped on the core material to form lactic acid bacteria microcapsules. According to the different solubility of encapsulation materials in water, the phase separation method can be divided into water phase separation method and oil phase separation method. However, the existing phase separation metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02A23P1/04A23P10/30
Inventor 雷虹平文祥董晓霞孙庆申单毓钰张广臣何欣
Owner 广州万物生健康产业有限公司
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