Bacterial strain for repairing chromium slag contaminated site

A site remediation and strain technology, applied in the field of chromium-reducing functional bacteria

Inactive Publication Date: 2010-03-31
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Bacterial strain for repairing chromium slag contaminated site
  • Bacterial strain for repairing chromium slag contaminated site
  • Bacterial strain for repairing chromium slag contaminated site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Separation, purification and domestication of the chromium reducing bacteria of the present invention

[0012] Collect the soil under the chromium residue storage site of Hunan Ferroalloy Plant, weigh 10g of the chromium residue soil into a 150mL sterilized Erlenmeyer flask, add 10mL sterilized liquid medium (5g / L sodium lactate, 5g / L yeast extract and 2g / L Sodium chloride, the rest is water, use 5mol / L NaOH to adjust pH=9.5~9.8), culture in 30℃ incubator for 4 days. Then take 1mL of mud and dilute it to 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 , Using a pipette from 10 -6 , 10 -7 And 10 -8 Take 0.1 mL of the diluent and place it with 250mg / L Cr(VI) (in K 2 Cr 2 O 7 The solution is used as the solid medium for the chromium source), and the solid medium is composed of 5 g / L sodium lactate, 5 g / L yeast extract, 2 g / L sodium chloride, and 15 g / L agar. Fully spread with a triangular rod, and place the plate upside down in a 30°C incubator for 4 days. Use a ste...

Embodiment 2

[0015] Example 2: Bacteriological characteristics and biological identification of strains

[0016] Take a little drop of the bacteria liquid on the glass slide, allow the bacteria to attach for 15 minutes, put it in a 2.5% glutaraldehyde solution and fix for 1 hour, dehydration with an alcohol gradient of 30% to 70%, replace with isoamyl acetate for 30 minutes, and dry at the critical point , Ion sputtering, scanning electron microscope observation and taking pictures, see attached figure 1 .

[0017] 1. Morphological characteristics

[0018] (1) Colony morphology characteristics: the liquid medium with chromium-reducing bacteria is inoculated into a solid medium containing 250mg / L of hexavalent chromium by the streaking plate method, and the plate is placed upside down in a 30°C constant temperature incubator. After 3 to 4 days, observe the morphology of the colony. On the plate with Cr(VI), the colony is blue-gray, round, with neat edges, and a protrusion in the middle. figure 1 ...

Embodiment 3

[0030] Example 3: Reduction of Cr(VI) by chromium reducing bacteria

[0031] Bacterial reduction Cr(VI) conditions: medium components: 5g / L sodium lactate, 5g / L yeast extract, 2g / L sodium chloride; adjust pH=9.5~9.8 with 5mol / L NaOH; temperature 30℃.

[0032] Use an inoculating loop to pick the purified colony into an Erlenmeyer flask filled with 100 mL sterile culture medium. Incubate at 30°C and a 150 rpm shaker for 24 hours. Then, use a sterile pipette to transfer 10 mL of the overnight growth , Inoculate into 100mL sterile culture medium with hexavalent chromium concentrations of 25, 50, 100, 150, 200, 300, 400 and 500 mg / L (with K 2 Cr 2 O 7 Solution as the source), incubate at 30°C, 150rpm, and shaker for 72h. During this period, at 0, 6, 12, 24, 36, 48, 60 and 72h after incubation, sample with a sterile syringe, centrifuge, and supernatant Carry out the determination of residual hexavalent chromium, the results of the determination of Cr(VI) in each period are as attached F...

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Abstract

The invention discloses a bacterial strain Pannonibacterphragmitetus BB for repairing a chromium slag contaminated site, which has the preserved number of CGMCC No. 3052. The inventor collects contaminated soil of a chromium slag scrapyard in a Hunan ferroalloy work to obtain the bacterial strain with the hexavalent chromium reducing characteristic through screening, separation and acclimatization. The bacterial strain has the function of reducing the hexavalent chromium to trivalent chromium, is used for treating chromium contamination, and has low cost and simple operation.

Description

Technical field [0001] The invention relates to a strain used for environmental pollution treatment, in particular to a chromium-reducing functional strain. Background technique [0002] Hexavalent chromium is a carcinogenic, teratogenic and mutagenic substance with high toxicity. Chromium pollution in the environment mainly comes from the discharge of chromium-containing wastewater from electroplating, tanning, painting, printing and dyeing industries, and the solid waste generated by the metal chromium and chromium salt production industry-chromium slag. The method of microbial remediation of chromium pollution has attracted increasing interest of environmental protection workers due to its low cost, no secondary pollution and simple operation. The isolation and acquisition of efficient chromium-reducing bacteria has always been a work that researchers are committed to carrying out. There are many reports in this area at home and abroad. There are many kinds of bacteria that ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/43
Inventor 柴立元杨志辉彭兵王云燕闵小波王海鹰黄顺红苏长青王兵
Owner CENT SOUTH UNIV
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