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Immune protective agent of attenuated living vaccine against infectious bursal disease and preparation method thereof

A technology for chicken infectivity and immune protection, applied in antiviral agents, pharmaceutical formulas, virus antigen components, etc., can solve the problem that protein protective agents do not have the function of immune adjuvant, and achieve the effect of improving the control effect

Active Publication Date: 2010-04-07
LIAONING YIKANG BIOLOGICAL CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, protein protectants do not have the function of immune adjuvants

Method used

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  • Immune protective agent of attenuated living vaccine against infectious bursal disease and preparation method thereof
  • Immune protective agent of attenuated living vaccine against infectious bursal disease and preparation method thereof
  • Immune protective agent of attenuated living vaccine against infectious bursal disease and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1. Preparation of Chinese medicine polysaccharide

[0074] Chinese herbal polysaccharides were extracted from the following weights of Chinese herbal medicines:

[0075] Codonopsis 30 kg, Atractylodes macrocephala 20 kg, Polyporus 15 kg, Alisma 15 kg, Poria cocos 10 kg, plantain 10 kg.

[0076] The extraction method is as follows:

[0077] a. After chopping and washing the raw materials of the traditional Chinese medicine prescription, soak them in cold water for 2 hours until the drug core is fully soaked, then add water 12 times the weight of the raw materials, and heat to boiling at 90°C. Under the reflux concentration process, decoct for 180 minutes and concentrate at the same time to obtain concentrated medicinal liquid.

[0078] b. Centrifuge the concentrated medicinal solution prepared in a. at 10,000 rpm for 20 minutes, discard the precipitate, obtain the supernatant and concentrate it under negative pressure to form a concentrated medicinal solutio...

Embodiment 2

[0080] Embodiment 2. Preparation of traditional Chinese medicine saponins

[0081] The process is as follows:

[0082] After chopping and washing the raw materials of the traditional Chinese medicine prescription described in Example 1, extract with ethanol or methanol, recover the solvent, take the residue cake and dissolve or suspend it in water to make an aqueous solution, and then do the following two-way extraction and purification:

[0083] a. Add petroleum ether or ether to the aqueous solution, recover the lipophilic pigment and oil in the aqueous solution, and leave the traditional Chinese medicine saponin in the aqueous solution;

[0084] B, add the stronger organic solvent butanol of hydrophilicity in the aqueous solution that a step makes, in the solution, the material with stronger hydrophilicity continues to stay in aqueous solution, and traditional Chinese medicine saponin is transferred in the organic solvent extraction liquid, collects organic solvent The ext...

Embodiment 3

[0086] Embodiment 3. synthetic chicken infectious bursa attenuated live vaccine immune protection agent of the present invention

[0087] Mix the Chinese medicine polysaccharide prepared in Example 1, the Chinese medicine saponin prepared in Example 2, and the commercially available casein according to the following ratio: 8 kilograms of Chinese medicine polysaccharide, 1 kilogram of Chinese medicine saponin, and 1 kilogram of casein, and then add 5 times aseptic detoxification Diluted with ionized water, cut and homogenized at a high speed of 10,000 rpm, then frozen or spray-dried to make a powder, which is the immunoprotective agent of chicken infectious bursal attenuated live vaccine of the present invention.

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PUM

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Abstract

The invention provides an immune protective agent of an attenuated living vaccine against infectious bursal disease, which consists of the following components in part by weight: 8 parts of Chinese medicinal polysaccharide, 1 part of Chinese medicinal saponin and 1 part of casein, wherein the Chinese medicinal polysaccharide and the Chinese medicinal saponin are extracted from the following Chinese medicinal raw materials in part by weight: 30 parts of szechwon tangshen root, 20 parts of largehead atractylodes rhizome, 15 parts of agaric, 15 parts of oriental waterplantain rhizome, 10 parts of Indian buead and 10 parts of plantain herb. The immune protective agent is mainly embodied in that specific Chinese medicinal effective components guide immunogen to generate specific immunoreactions on a specific organism part, is also embodied in that the immune protective agent has specific protection effect on the immunogen in the whole process of processing, storing and using the vaccine, and has a scientific formula for integrating channel tropism of Chinese medicines, immunoadjuvant and immunogen protective agent for the first time.

Description

technical field [0001] The invention relates to an immune protection agent, in particular to an immune protection agent specially for chicken infectious bursa attenuated live vaccine, and a preparation method thereof. Background technique [0002] Morein proposed the immune stimulating complex (ISCOM) for the first time, which is a brand-new immune adjuvant, composed of saponins, cholesterol, phospholipids, etc. other saponin based adjuvants (J). Adv Drug Deiv Rev, 1998, 32(3): 247-271). This adjuvant is characterized by highly efficient presentation of the immunogen to monocyte-macrophages via saponins, laying a strong foundation for the immune response. [0003] Traditional immune adjuvants are enriched with immunogens (such as aluminum gel), slow-released immunogens (such as oil emulsion), activated mononuclear phagocytes to present antigens (such as BCG, bacterial lipopolysaccharide), and various comprehensive effects (such as without complete Freund's adjuvant and com...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/36A61K47/26A61K39/39A61K36/884A61K39/12A61P31/14C08B37/00
Inventor 苗玉和李尚波张亚坤胥桂华张勇飞蒋玉文武洁梅米宏有高英杰
Owner LIAONING YIKANG BIOLOGICAL CORP LTD