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Microchip analysis system for capillary electrophoresis separation and chemiluminescence detection

Inactive Publication Date: 2010-04-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, compared with laser-induced fluorescence detectors, chemiluminescence detectors have not been widely used in microfluidic chip analysis systems, and there are no commercial instruments integrating chip capillary electrophoresis separation and chemiluminescence detection.

Method used

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  • Microchip analysis system for capillary electrophoresis separation and chemiluminescence detection
  • Microchip analysis system for capillary electrophoresis separation and chemiluminescence detection
  • Microchip analysis system for capillary electrophoresis separation and chemiluminescence detection

Examples

Experimental program
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Effect test

Embodiment 1

[0020] see figure 1 , the microfluidic chip 1 has a buffer solution reservoir B, a sample solution reservoir S, a sample waste solution reservoir SW, a sheath flow reservoir L and H, and a waste solution reservoir W. Among them, the microfluidic chip injection channel is S-SW, and the separation channel is B-P 2 , the injection channel intersects the separation channel P 1 , the separation channel intersects the sheath flow channel P 2 , P 1 and P 2 The channels between are effectively separated by channel P 1 -P 2 . Effective separation channel P 1 -P 2 There are microporous plugs 2, sheath flow reservoirs L and H and separation channel connection point P 2 Sheath channel L-P 2 and sheath channel H-P 2 , separating channel terminal P 2 and the channel between the waste pool W is the detection channel P 2-W, the photomultiplier tube is placed directly below the detection channel to detect the light intensity emitted by the chemiluminescent reaction. The negative ...

Embodiment 2

[0027] According to Example 1, an example of analyzing heavy metal ions in a water sample with a microchip analysis system is provided. see figure 1 , the channel between the sample reservoir S and the sample waste reservoir SW on the microfluidic chip 1 is the sampling channel, P 1 and P 2 The channel between is the effective separation channel, the effective separation channel P 1 -P 2 Microporous plug 2, P 2 The channel between W and W is a detection channel, and the photomultiplier tube is placed directly under the detection channel to detect the light intensity emitted by the chemiluminescent reaction. Make small holes at both ends of the channel, and fix three plastic reservoirs with an inner diameter of 5.0mm and a height of 15.0mm around the holes S, SW and B with epoxy glue; the three inner diameters are 7.0mm, and the height Plastic reservoirs with a diameter of 25.0 mm are respectively fixed around the holes L, H, and W as their reservoirs. Reservoirs S, SW, B...

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Abstract

The invention provides a microchip analysis system integrated capillary electrophoresis separation with chemiluminescence detection, which consists of a microfluidic chip, a single-path high voltage power source, a minipump, a three-way valve, an interface, a needle adjusting valve, a U-tube type monometer, a photomultiplier, a vacuum flask, an electric contact vacuum gauge and a time relay. The microchip analysis system provided by the invention has simple structure, no mutual interference of the capillary electrophoresis separation and chemiluminescence detection, and high separation efficiency of the capillary electrophoresis and high sensitivity of the chemiluminescence, has the characteristics of high separation efficiency, high detection sensitivity, small volume, light weight, convenient operation, low cost and the like, and is an ideal portable microchip analysis system.

Description

technical field [0001] The invention relates to a micro-total analysis system, in particular to a portable micro-chip analysis system integrating chip capillary electrophoresis separation and chemiluminescence detection. Background technique [0002] Since the concept of micro-total analysis system was proposed in 1990, microfluidic chip technology has opened up a broad space for development in the fields of chemistry, life science, environmental science and food science. Because chip capillary electrophoresis has the advantages of high separation efficiency, easy portability, less reagent consumption, and short analysis time, it is a commonly used separation technology in microchip analysis systems. However, the detection volume of chip capillary electrophoresis is small and the optical path is short, so the detector used with it must have extremely high sensitivity. [0003] Due to the high sensitivity of the laser-induced fluorescence detector and the low technical diffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N1/28
Inventor 殷学锋王修中
Owner ZHEJIANG UNIV
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