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Extraction method of shark chondroitine

A chondroitin sulfate and extraction method technology, which is applied in the field of new bioengineering, can solve the problems that the enzyme cannot be used repeatedly, cannot be continuously produced, and the technical process is cumbersome, so as to achieve large-scale industrial production, easy operation, and simple technical process. Effect

Active Publication Date: 2010-05-05
浙江澳兴生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method not only has many process steps, but also has a cumbersome process; moreover, after enzymolysis, it needs to heat up to deactivate and filter the enzyme, so the enzyme cannot be used repeatedly, and continuous production cannot be carried out.

Method used

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  • Extraction method of shark chondroitine
  • Extraction method of shark chondroitine
  • Extraction method of shark chondroitine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Select 200g of shark head cartilage, cook in boiling water for 2-6 hours, rinse well, soak in ethanol or acetone for 30 minutes to remove oil. The degreased shark cartilage was broken into pieces, 800g of 5% NaOH solution and 800g of 3% salt solution were added, and the alkali extraction was intermittently stirred at a temperature of 20°C for 10 hours, filtered, and the residue was soaked in distilled water and filtered, combined Twice the filtrate and adjust the pH to 8.0 with HCl.

[0036] Papain was selected, and it was made into 4 g of immobilized papain by common cross-linking immobilization method. Among them, chitosan was used as carrier and glutaraldehyde was used as cross-linking agent for immobilization. Such as figure 2 As shown, in the fluidized bed reactor 1, immobilized papain is added, and the filtrate after alkali extraction is added to the enzymolysis circulation tank 3 and injected into the fluidized bed reactor 1 by the enzymolysis circulation pump...

Embodiment 2

[0044] Select 100g of shark tail cartilage and 100g of shark fin cartilage, cook in boiling water for 2-6 hours, rinse, soak in ethanol or acetone for 30 minutes to remove grease. The degreased shark cartilage is broken into pieces, add 1000g of 3% NaOH solution and 1000g of 2% salt solution, stir intermittently at 30°C for alkali extraction for 6 hours, filter, soak and filter the residue with appropriate amount of distilled water, and combine Twice the filtrate and adjust the pH to 9.0 with HCl.

[0045] Papain and subtilisin were selected and prepared into 4 g of immobilized papain and 2 g of immobilized subtilisin by ordinary embedding and immobilization methods, and packed into columns respectively. Among them, chitosan and sodium alginate were used as carriers for immobilization. The enzymatic hydrolysis process is the same as in Example 1, and will not be repeated. During the enzymatic hydrolysis, the pH was adjusted to 8.5 with sodium hydroxide solution. The new alk...

Embodiment 3

[0049] Select 50g of shark tail cartilage, 50g of shark vertebral cartilage, and 100g of shark fin cartilage, cook in boiling water for 2-6 hours, rinse well, and soak in ethanol or acetone for 30 minutes to remove grease. The degreased shark cartilage was broken into fragments, 1200g of 2% NaOH solution and 1200g of 1% salt solution were added, and the temperature was 40°C for intermittent stirring for alkali extraction for 3 hours, filtered, and the residue was soaked in distilled water and filtered, combined Twice the filtrate and adjust the pH to 8.5 with HCl.

[0050] Pepsin was selected, and it was made into 10 g of immobilized pepsin by common embedding and immobilization method. Among them, sodium alginate was used as the carrier for embedding and immobilization. The enzymatic hydrolysis process is the same as in Example 1, and will not be repeated. During the enzymatic hydrolysis, the pH was adjusted to 2.0 with hydrochloric acid solution. The new alkaline extract ...

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Abstract

The invention provides an extraction method of shark chondroitine, belonging to the field of novel biology engineering technology; the extraction method solves the problems that the existing chondroitine has multiple extraction steps, complex process flows, low product yield, high protein and nitrogen impurity content; the extraction method of shark chondroitine comprises the following steps: A: alkali extraction; B. enzymolysis; C. ultrafiltration; D. oxidization and crystallization. The extraction method of shark chondroitine has simple process flow, less process steps and easy operation and can realize large-scale industrial production, the product yield can reach 17-22 percent and the purity can reach 90-96 percent.

Description

technical field [0001] The invention relates to a method for extracting chondroitin sulfate, in particular to a method for extracting shark chondroitin sulfate; it belongs to the technical field of novel bioengineering. Background technique [0002] Chondroitin sulfate is a mucopolysaccharide extracted from animal tissues, and its basic units are D-glucuronic acid and N-acetyl-D-galactosamine sulfate. Generally, chondroitin sulfate contains about 50-70 disaccharide units, with an average molecular weight of 10,000-30,000. The molecule has a large number of negative charges, and has lipid-lowering, anti-inflammatory and weak anti-coagulant and anti-thrombotic biological activities, and plays an important role in maintaining the relative stability and normal function of the cell environment. [0003] Chondroitin sulfate can promote the growth of fibers in the matrix, enhance permeability, improve blood circulation, accelerate metabolism, promote the absorption of permeate and...

Claims

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Application Information

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IPC IPC(8): C08B37/08C12S3/02
Inventor 殷文静王松叶詹金明陈志伟
Owner 浙江澳兴生物科技有限公司
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