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Preparation of anti-coagulation dermis scaffold

A technology of dermis and preparation steps, which is applied in the field of medical devices, can solve the problems of ingrowth, blood coagulation contact, unfavorable capillaries, etc., and achieve the effect of simple preparation method, maintaining activity and reducing blood coagulation

Active Publication Date: 2010-05-12
SHENZHEN QIKANG MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the case of a large area of ​​skin trauma, when it needs to be repaired with the assistance of artificial skin, if a large amount of blood coagulation occurs, scars appear, and the dermis of the artificial skin is in contact with necrotic tissue, it is not conducive to the growth of capillaries into the scaffold.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The ratio of silk fibroin and chitosan is 1:3, and the artificial dermal scaffold containing heparin 0.01% silk fibroin / chitosan / heparin is prepared as follows:

[0039] 1. Prepare a 0.2M acetic acid solution. Dissolve 1.2 g of acetic acid in deionized water and titrate to 100 mL to obtain a 0.2 M acetic acid solution.

[0040] 2. Prepare the heparin solution. Dissolve 0.16 mg of heparin in 40 mL of the acetic acid solution prepared in step 1, and titrate with acetic acid to the pH of the solution before heparin was added.

[0041] 3. Prepare a 3% chitosan / heparin solution. Take 40 mL of the heparin solution prepared in step 2 and dissolve 1.2 g of chitosan to obtain a milky white viscous liquid.

[0042] 4. Prepare silk fibroin solution. The concentration of silk fibroin in the solution was made 4%.

[0043] 5. Prepare silk fibroin / chitosan / heparin mixed solution. Add 40 mL of acetic acid / heparin solution prepared in step 3 into 9 mL of silk fibroin / chitosan mixe...

Embodiment 2

[0048] The ratio of silk fibroin and chitosan is 3:1, and the artificial dermal scaffold containing heparin 0.04% silk fibroin / chitosan / heparin is prepared as follows:

[0049] 1. Prepare a 0.2M acetic acid solution. Dissolve 1.2 g of acetic acid in deionized water and titrate to 100 mL to obtain a 0.2 M acetic acid solution.

[0050] 2. Prepare the heparin solution. Dissolve 2 mg of heparin in 40 mL of the acetic acid solution prepared in step 1 and titrate with acetic acid to the pH of the solution before heparin was added.

[0051] 3. Prepare 3% chitosan / heparin solution. Take 40 mL of the heparin solution prepared in step 2 and dissolve 1.2 g of chitosan to obtain a milky white viscous liquid.

[0052] 4. Prepare silk fibroin solution. The concentration of silk fibroin in the solution was made 4%.

[0053] 5. Prepare silk fibroin / chitosan / heparin mixed solution. Add 40ml of chitosan / heparin solution prepared in step 3 to 80ml of silk fibroin solution prepared in step 4,...

Embodiment 3

[0058] The ratio of silk fibroin and chitosan is 1:1, and the artificial dermal scaffold containing heparin 0.1% silk fibroin / chitosan / heparin is prepared as follows:

[0059] 1. Prepare a 0.2M acetic acid solution. Dissolve 1.2 g of acetic acid in deionized water and titrate to 100 mL to obtain a 0.2 M acetic acid solution.

[0060] 2. Prepare the heparin solution. Dissolve 2.4 mg of heparin in 40 mL of the acetic acid solution prepared in step 1 and titrate with acetic acid to the pH of the solution before the addition of heparin.

[0061] 3. Prepare a 3% chitosan / heparin solution. Take 40 mL of the heparin solution prepared in step 2 and dissolve 1.2 g of chitosan to obtain a milky white viscous liquid.

[0062] 4. Prepare silk fibroin solution. The concentration of silk fibroin in the solution was made 4%.

[0063] 5. Prepare silk fibroin / chitosan / heparin mixed solution. Add the chitosan / heparin solution prepared in step 3 to 30 mL of the silk fibroin solution prepar...

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Abstract

The invention relates to a preparation of anti-coagulation dermis scaffold which is used for skin injury repair and has good three-dimensional porous structure. The scaffold preparation uses three natural biologicals of regeneration silk fibroin, chitosan and heparin as raw materials, wherein, heparin has outstanding anti-coagulation property. The dermis scaffold has high porosity and aperture size appropriate for dermal cells to grow; the scaffold has better mechanical property and good hydrophily; the artificial dermis scaffold has heparin slow release function, so that the protrombin time, partial thromboplastin time and thrombin time thereof can be obviously prolonged, thus proving that the dermis scaffold has excellent anti-coagulation property.

Description

technical field [0001] The invention belongs to the field of medical devices. More specifically, the present invention relates to a preparation method of an artificial dermal scaffold with good three-dimensional porous structure and anticoagulant properties for skin damage repair. technical background [0002] The skin is the largest organ of the human body and has the functions of retaining moisture in the human body, preventing excessive heat dissipation, ventilating and preventing bacterial invasion. When the human body is damaged by trauma, burns, inflammation, etc., especially when a large area of ​​skin is severely burned or damaged, the wound should be protected immediately. If only a superficial or small area of ​​the skin is damaged, the new skin will regenerate on its own, but if there is a severe trauma, the skin cannot repair on its own, and usually the superficial skin from other parts of the body must be grafted onto the wound. [0003] Since there is no alte...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/54A61L27/56A61L27/60A61L33/10
Inventor 佘振定王明波谭荣伟
Owner SHENZHEN QIKANG MEDICAL DEVICES
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