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Method for treating xylose mother solution

A xylose mother liquor, processing method technology, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of insufficient utilization of glucose and arabinose, low yield of fermented sugar alcohol, production of Process complexity and other problems, to achieve the effect of reducing the inhibition effect, improving the utilization rate, and simplifying the production process

Active Publication Date: 2010-05-12
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, xylitol yeast strains can use the xylose and convert it into xylitol, but if the xylose mother liquor is directly used as the medium for xylitol fermentation, the abundant glucose in the mother liquor will form during the fermentation process. Secondary metabolites will seriously inhibit the conversion of xylose into xylitol, resulting in a low yield of fermented sugar alcohol, so the concentration of glucose in the xylitol fermentation medium must be controlled
[0004] At present, the main ways to utilize xylose mother liquor are as follows: 1. Utilize xylose mother liquor to produce xylose and xylitol. The production process is complicated, and xylose and arabinose and other impurities need to be separated. Glucose and arabinose are not fully utilized; 2. The production process of arabinose by using xylose mother liquor is complex and requires further treatment of xylose mother liquor; 3. The production of furfural by using xylose mother liquor involves high temperature and high pressure synthesis conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of alcohol fermentation medium: xylose mother liquor 20% (m / v), yeast powder 0.5% (m / v), corn steep liquor 1.0% (m / v), potassium dihydrogen phosphate 0.05% (m / v), sulfuric acid Magnesium 0.075% (m / v), dilute tap water to 1.08L, pH natural. A 5L fermenter was used.

[0038] The alcoholic fermentation medium was sterilized at 110° C. for 15 minutes, and when cooled to 30° C., 0.12 L of cultivated genetically engineered yeast liquid seeds (S. cerevisiae ZU-10) were added. Sterile air was introduced at a flow rate ratio of 1:0.6, the stirring speed was 300 rpm, and cultured at 30°C for about 9 hours. The optical density (O.D.) value of the cell concentration was 40.3. Stop the ventilation, maintain the stirring speed, and continue the cultivation. 33 DEG C, control the glucose concentration of fermentation medium in the scope of 1~2% (m / v) by feeding the mode of adding xylose mother liquor, stop feeding xylose mother liquor when the fermented mash volume reache...

Embodiment 2

[0042] Prepare xylose mother liquor 20% (m / v), yeast powder 0.5% (m / v), corn steep liquor 1.0% (m / v), potassium dihydrogen phosphate 0.05% (m / v), magnesium sulfate 0.075% (m / v), tap water is settled to 1.08L, the 5L fermenter alcoholic fermentation culture medium of pH natural.

[0043]Sterilize at 110° C. for 15 minutes, and insert 0.12 L of cultured genetically engineered yeast liquid seeds (S. cerevisiae ZU-10) when cooled to 30° C. Introduce sterile air at a flow rate ratio of 1:0.6, stir at 300 rpm, and incubate at 30°C for about 11 hours. The optical density (O.D.) value of the cell concentration is 58.8. Stop aeration, maintain the stirring speed, and raise the culture temperature to 33°C. Control the glucose concentration of the fermentation medium in the range of 1 to 2% (m / v) by feeding the xylose mother liquor, stop feeding the xylose mother liquor when the fermented mash volume reaches 3.5L, and continue to cultivate until the fermentation ends.

[0044] Measured...

Embodiment 3

[0047] Prepare xylose mother liquor 15% (m / v), yeast powder 0.5% (m / v), corn steep liquor 1.0% (m / v), potassium dihydrogen phosphate 0.05% (m / v), magnesium sulfate 0.075% (m / v), tap water is settled to 0.96L, and the 5L fermenter with natural pH uses alcoholic fermentation medium.

[0048] Sterilize at 110°C for 15 minutes, and when cooled to 30°C, insert 0.24L of cultured genetically engineered yeast liquid seeds (S.cerevisiae ZU-10) for cultivation, first pass sterile air at a flow rate ratio of 1:0.6, and stir Rotate at 300rpm, culture at 30°C for about 9 hours, the optical density (O.D.) value of the cell concentration is 52.9, stop the aeration, maintain the stirring speed, raise the culture temperature to 33°C, and control the glucose in the fermentation medium by adding xylose mother liquor When the concentration is within the range of 1-2% (m / v), the feeding of xylose mother liquor is stopped when the volume of fermented mash reaches 3.5 L, and the cultivation is cont...

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Abstract

The invention relates to a method for treating xylose mother solution. The method comprises the following steps: firstly, using glucose in the alcohol yeast strain fermented xylose mother solution to prepare alcohol by distilling; and then, using xylose in the xylose yeast strain fermented mother solution to prepare xylitol by extracting, and finally extracting gum sugar. The method changes the xylose mother solution into valuable by using the two-step fermentation method, so on the one hand, the method reduces the inhibiting effect brought about by excessive glucose during producing the xylitol by fermenting, and on the other hand, improves the utilization rates of the glucose and the xylose mother solution, and greatly reduces the COD treated as wastewater at the same time; and the production process is simple, safe and energy-saving.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular, the invention relates to a treatment method of xylose mother liquor. Background technique [0002] Xylose is an important functional food additive and sweetener. Because of its unique chemical properties and physiological functions, it is especially suitable as a therapeutic additive for patients with diabetes and obesity. At present, xylitol is mainly produced by chemical The method uses corncobs, bagasse and other raw materials rich in pentosan, which are acid-hydrolyzed into a hydrolyzate containing xylose, and then xylose is extracted from the hydrolyzate through the steps of neutralization, decolorization, ion exchange, and crystallization. [0003] Xylose mother liquor is a waste liquid after processing xylose, which is rich in glucose, xylose, arabinose and galactose. Among them, xylose and arabinose are five-carbon sugars, which are difficult to be utilized...

Claims

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Application Information

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IPC IPC(8): C12P7/08C12P7/18C07H3/02C07H1/06A23K1/00C12R1/74C12R1/865A23K10/18
Inventor 李荣杰尚海涛邓远德郑辉陈龙泉
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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