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Compound beta-mannase and lactobacillus feed additive

A feed additive and mannanase technology, applied in the field of poultry feed additives, can solve problems such as the impact of feeding effects, achieve the effects of improving the body's immunity, reducing feed costs, and requiring less cultivation and storage conditions

Active Publication Date: 2010-06-02
北京伟嘉盛邦生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They are all just a mixture of pure enzyme preparations, which are not superimposed with the probiotic effect of lactic acid bacteria, so the feeding effect is affected

Method used

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  • Compound beta-mannase and lactobacillus feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of embodiment 1 Enterococcus faecium fermentation broth

[0053] Enterococcus faecium Ef1008 (preserved on December 23, 2009 in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC No.3537) bacterial classification received MRS medium ( The composition and content of the MRS medium are: peptone 10g, beef extract 10g, yeast extract 5g, K 2 HPO 4 2g, diammonium hydrogen citrate 2g, glucose 20g, sodium acetate 5g, Tween-80 1mL, MgSO 4 ·7H 2 O 0.58g, MnSO 4 4H 2 (0.25g, 1000mL distilled water, adjust pH to 7.2 with 1mol / L NaOH, sterilize at 115°C for 30min), cultivate at 37°C and 160r / min for 9 hours to obtain Enterococcus faecium fermentation liquid; the activity of the obtained fermentation liquid Bacteria ≥ 5×10 10 CFU / mL.

Embodiment 2

[0054] The preparation of embodiment 2 compound β-mannanase and lactic acid bacteria feed additive

[0055] Proceed as follows:

[0056] According to the following parts by weight, get β-mannanase (purchased from Feed Research Institute of Chinese Academy of Agricultural Sciences), enzyme activity is 1700U / g) 4, xylanase (purchased from Wuhan Xinhua Yang Biotechnology Co., Ltd., enzyme activity is ≥50000U / g)) 27, β-glucanase (purchased from Wuhan Xinhuayang Biotechnology Co., Ltd., enzyme activity ≥ 8000U / g) 13, cellulase (purchased from Wuhan Xinhuayang Biotechnology Co., Ltd., enzyme Activity is ≥ 10000U / g) 31, pectinase (purchased in Wuhan Xinhua Yang Biotechnology Co., Ltd., enzyme activity is ≥ 20000U / g) 24, the Enterococcus faecium fermentation broth prepared in Example 1 (the viable bacteria in the fermentation broth is ≥ 5×10 10 CFU / mL) 101; Mix the above enzymes evenly, then mix the Enterococcus faecium fermentation broth with the compound β-mannanase evenly, and th...

Embodiment 3

[0057] The preparation of the laying hen feed of embodiment 3 compound β-mannanase and lactic acid bacteria preparation

[0058] According to the basal ration components and premix in Table 1, each component was mixed to obtain the basal ration.

[0059] Table 1 Basic diet components and nutrients

[0060]

[0061] Premix (purchased from Beijing Weijiaren Biotechnology Co., Ltd., compound premix feed 3210 for 1% laying hens) was added in an amount of 1%, wherein each kilogram of premix provided: Vitamin D 3 200,000 to 400,000 IU, 1,300 to 3,000 IU for vitamin E, and 1,300 to 3,000 IU for vitamin K 3 200-400mg, vitamin B 1 ≥ 160mg, vitamin B 2 ≥700mg, vitamin B 6 ≥300mg / Kg, Vitamin B 12 ≥1.8mg for biotin, ≥12mg for biotin, ≥2200mg for niacin, ≥900mg for pantothenic acid, and ≥70mg for folic acid.

[0062] Add 0.05% compound β-mannanase and lactic acid bacteria feed additive to the basal ration in the ration of the test group, and mix them evenly.

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Abstract

The invention discloses a compound beta-mannase and lactobacillus feed additive. The feed additive comprises the following components in part by weight: beta-mannase 2-8, xylanase 24-30, beta-glucanase 9-15, cellulose 28-33, pectase 20-27 and Enterococcus faecium fermentation liquor 90-110. The invention also discloses an Enterococcus faecium strain EF1008. The feed additive of the invention can be added in feed for egg-laying chickens in a proportion of 0.03 to 0.1 percent. The feed additive can improve the egg laying rate of the egg-laying chickens and can be used at a high rate by animals and reduce the cost of the feed. In the feed additive, the Enterococcus faecium can promote the micro ecological balance in the intestinal tract, improve body immunity and reduce the death rate of livestock in a stressful environment.

Description

technical field [0001] The invention belongs to the field of poultry feed additives, in particular to a compound β-mannanase and lactic acid bacteria feed additive and its application in laying hen feed. Background technique [0002] At present, most laying hen feed materials contain more anti-nutritional factors, which not only affects the digestion and utilization rate of animals, but also causes waste of feed resources and environmental pollution. In addition, egg products also have problems such as drugs, hormones, toxic and harmful substances exceeding the standard, and the deterioration of the breeding environment, which has become a bottleneck affecting the sustainable development of my country's laying hen farming industry. Research, development and utilization of "green", "safe" and "environmentally friendly" feed should be an effective way to solve the above problems. [0003] The Chinese patent "Unconventional Protein Compound Chicken Feed for Adult Broilers" (ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K1/18A23K10/18A23K10/30A23K10/37A23K20/142A23K20/174A23K20/189A23K20/20A23K20/26A23K50/75
CPCY02P60/87
Inventor 王婷婷李冬青
Owner 北京伟嘉盛邦生物技术有限公司
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