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Tissue engineering microencapsulated hepatocyte and preparation and application thereof

A technology of tissue engineering and liver cells, which is applied in the fields of medical formula, digestive system, drug combination, etc., can solve the problems of high cost, lack of donor organs, etc., and achieve the effects of avoiding side effects, small size, and high biocompatibility

Inactive Publication Date: 2010-06-02
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these treatments can significantly improve the patient's symptoms, there are still many problems: For example, liver organ transplantation is limited by the shortage of donor organs, high costs and long-term use of immunosuppressants
[0006] At present, there are no relevant reports on the treatment of liver failure with microencapsulated hepatocytes and vascular endothelial cells at home and abroad

Method used

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  • Tissue engineering microencapsulated hepatocyte and preparation and application thereof
  • Tissue engineering microencapsulated hepatocyte and preparation and application thereof
  • Tissue engineering microencapsulated hepatocyte and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Preparation of Tissue Engineering Microencapsulated Hepatocytes

[0032] (1) Isolation and culture of SD rat hepatocytes

[0033] Adult Sprague-Dawley rats (250 g, male) were anesthetized with sodium pentobarbital, and the abdominal wall skin and muscles of the rats were cut; the gastrointestinal tract was opened to expose the portal vein and inferior vena cava. Infuse 250mL of PBS solution containing 12500U / L heparin and 500mL of preperfusion solution at one time. When the preperfusion solution is perfused to the surface of the liver and turns yellow, the postperfusion solution containing 0.05wt% type IV collagenase is perfused to the subcapsular tissue of the liver. Turtle-shaped cracks. The complete liver was removed, the capsule and fiber components were torn off with ophthalmic tweezers, the liver cells were separated by blunt scraping and blowing repeatedly, filtered through a 200-mesh filter, and the liver cell suspension was prepared and rinsed with P...

Embodiment 2

[0038] Embodiment 2 Establishment and treatment of acute liver failure animal model

[0039] SD rats (250g, female) were injected intraperitoneally with a ratio of 1.2g D-galactosamine per kilogram of body weight to induce acute liver failure model. Various enzymes in the blood are released into the blood, resulting in an increase in enzyme indicators. The natural course of this acute liver failure model is 2 weeks (judgment criteria: ALT, AST are significantly higher than normal values, and ALB is significantly lower than normal values);

[0040] Within 24 hours of acute liver failure, 5ml of tissue-engineered microencapsulated hepatocytes prepared in Example 1 were transplanted into the abdominal cavity of rats with liver failure. This experimental group was called the co-encapsulation group. Three control groups were set at the same time, respectively Empty capsule group, free hepatocyte group and microencapsulated hepatocyte group. In the empty capsule group, APA microcap...

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Abstract

The invention discloses a tissue engineering microencapsulated hepatocyte and a preparation method thereof, belonging to the field of animal cell tissue engineering. The preparation method comprises the following steps of: adding hepatocytes and vascular endothelial cells in the quantity ratio of 1: (0.5-1) into water solution of sodium alginate for mixing to prepare a cell suspension, wherein the mass percentage concentration of the water solution of sodium alginate is 1.5-2; and then preparing sodium alginate-polylysine-sodium alginate microencapsulated hepatocyte by adopting a high-voltage static microcapsule generator. The invention also discloses the application of the tissue engineering microencapsulated hepatocyte in preparing hepatic failure medicaments; the prepared microencapsulated hepatocyte has the characteristics of small volume, high biocompatibility and favorable permeability, is suitably used as a vector for cell transplantation, and can exert in a host body for a long term so as to avoid the side effects caused by repeatedly using chemical medicaments; in addition, the microencapsulated hepatocyte has simple preparation process, thereby being suitable for large-batch production.

Description

technical field [0001] The invention relates to animal cell tissue engineering products, more specifically, to a tissue engineering microencapsulated hepatocyte and its preparation method and application. Background technique [0002] Liver failure is a clinically refractory disease with a high mortality rate. Currently, there are two main clinically feasible methods for the treatment of liver failure: liver transplantation and hepatocyte transplantation. Although these treatments can significantly improve the patient's symptoms, there are still many problems: for example, liver organ transplantation is limited by the shortage of donor organs, high costs and long-term use of immunosuppressants. Another effective method is liver cell transplantation. If a certain number of liver cells are successfully implanted in a patient with liver failure, the function of the liver will be improved and the regeneration of the liver will be significantly promoted. However, the body will ...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C12N11/04A61K35/407A61K35/12A61P1/16A61K35/44
Inventor 王常勇邱丽媛段翠密王滟濛林秋霞
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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