Separation method for preparing natural 2-phenylethyl alcohol by biotransformation method
A technology of biotransformation and separation method, which is applied in the field of separation of natural 2-phenylethanol prepared by biotransformation method, can solve problems such as insufficient source of raw materials and difficulty in meeting market demand, and achieve low material consumption, high industrial application feasibility, and production low cost effect
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Embodiment 1
[0031] The composition of medium used for Saccharomyces cerevisiae is as follows:
[0032] Plate medium: glucose 10g / L, peptone 5g / L, yeast extract powder 3g / L, 2-phenylethanol 3g / L, agar 20g / L, solvent is water, pH is natural, sterilized by high pressure steam at 121℃ for 20min.
[0033] Incline medium: glucose 15g / L, peptone 10g / L, yeast extract powder 5g / L, agar 20g / L, solvent is water, pH is natural, sterilized by high pressure steam at 121℃ for 20min.
[0034] Seed medium: glucose 20g / L, peptone 10g / L, yeast extract powder 5g / L, solvent is water, pH is natural, sterilized by high pressure steam at 121°C for 20min.
[0035] Transformation medium: sucrose 100g / L, yeast extract powder 5g / L, KH 2 PO 4 7.5g / L, K 2 HPO4 ·3H 2 O 12.6g / L, MgSO 4 ·7H 2 O 0.5g / L, the solvent is water, the pH is natural, and sterilized by high-pressure steam at 121°C for 20min.
[0036] Pick a ring full of Saccharomyces cerevisiae slant strains (culture of Angel Alcohol Active Dry Yeast, prod...
Embodiment 2
[0038] 200 mL of the transformed fermented liquid obtained by the method in Example 1 was centrifuged at 4500 r / min for 15 min, and the supernatant in the centrifuge cup was collected. The pH of the supernatant was measured at 5.5 without adjustment. The concentration of 2-phenylethanol determined by liquid chromatography was 4.63g / L.
[0039] The macroporous resin used in this example is D101 (produced by Nankai University Chemical Plant, with a particle size of 0.32 mm to 1.25 mm and a specific surface area of 500 m 2 / g~580m 2 / g), the pretreated D101 is packed into a chromatographic column of 10mm×300mm, the packing height is 250mm, and the resin bed volume is 20mL. After packing, rinse the packed column with 200mL distilled water and set it aside.
[0040] Take 110mL of the above-mentioned 2-phenylethanol fermentation supernatant, pass through the macroporous resin-filled column at a flow rate of 5BV / h, and then directly elute with 60mL of 75% acetone at a flow rate ...
Embodiment 3
[0042] 200 mL of the transformed fermented liquid obtained by the method in Example 1 was centrifuged at 4500 r / min for 15 min, and the supernatant in the centrifuge cup was collected. The pH of the supernatant was measured at 5.5 without adjustment. The concentration of 2-phenylethanol determined by liquid chromatography was 4.73g / L.
[0043] The macroporous resin used in this example is D101 (produced by Nankai University Chemical Plant, with a particle size of 0.32 mm to 1.25 mm and a specific surface area of 500 m 2 / g~580m 2 / g), the pretreated D101 is packed into a chromatographic column of 10mm×300mm, the packing height is 250mm, and the resin bed volume is 20mL. After packing, rinse the packed column with 200mL distilled water and set it aside.
[0044] Take 100mL of 2-phenylethanol conversion fermentation broth and put it on the column at a flow rate of 10BV / h, then rinse the resin with 100mL distilled water at a flow rate of 7.5BV / h. After the leaching finishes...
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