Method for preparing glucose-sensitive insulin controlled-release biological material

A glucose-sensitive biomaterial technology, applied in the field of preparation of dextran-concanavalin A-based insulin self-controlled release biocarrier materials, can solve problems such as difficult realization of large-scale production, and achieve easy preparation of large-scale production, The effect of mild conditions and rapid reaction process

Active Publication Date: 2013-01-23
ZHEJIANG UNIV OF TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this polymerization method is not yet easy to achieve large-scale production, and the sensitivity and self-controlled release performance of the obtained carrier system still need to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dissolve 5.4g dextran (molecular weight 10kDa) and 0.8g methacrylic anhydride in 80mL dimethyl sulfoxide, add 0.08g 4-dimethylaminopyridine, react at 48°C for 36h, use deionized water and The dialysis membrane was dialyzed for 7 days and freeze-dried to obtain a methallyl dextran monomer with a substitution degree of 16%.

[0021] Dissolve 1 g of concanavalin A and 0.1 g of methacrylic anhydride in 24 mL of phosphate buffer (20 mM, pH 7.4) and react at 51 °C for 2 h, then dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 8 kDa for 7 days, freeze-dried to obtain methallyl concanavalin A monomer.

[0022] Take 50 mg of the above-mentioned methallyl dextran monomer, 25 mg of the above-mentioned methallyl concanavalin A monomer, dissolve in 1.5 mL of phosphate-citric acid buffer (50 mM, pH 5), and obtain a single body solution; add 7.5 mg of ammonium persulfate and 9.4 mg of tetramethylethylenediamine to the monomer solution, react at ...

Embodiment 2

[0025] Dissolve 5.5g dextran (molecular weight 100kDa) and 1.3g methacrylic anhydride in 100mL dimethyl sulfoxide, add 0.26g 4-dimethylaminopyridine, react at 55°C for 12h, use deionized water and The dialysis membrane was dialyzed for 7 days and freeze-dried to obtain a methallyl dextran monomer with a substitution degree of 12%; the preparation of the methallyl concanavalin A monomer was the same as in Example 1.

[0026] Take 50 mg of the above-mentioned allyl dextran monomer and 50 mg of the above-mentioned methallyl concanavalin A monomer, and dissolve them in 1 mL of Tris-hydrochloric acid buffer (10 mM, pH 9) to obtain a monomer solution; Add 2mg of ammonium persulfate and 10mg of tetramethylethylenediamine to the monomer solution, react at 60°C for 5min, dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 10kDa for 3 days, remove unreacted substances, and obtain sensitive self-controlled release of glucose Biomaterials for insulin. ...

Embodiment 3

[0029] Dissolve 6 g of dextran (molecular weight 70 kDa) and 1.2 g of acrylic anhydride in 100 mL of dimethyl sulfoxide, add 0.18 g of 4-dimethylaminopyridine, react at 50 °C for 24 h, and dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 10 kDa After 7 days, it was freeze-dried to obtain an allyl dextran monomer with a substitution degree of 18%.

[0030] Dissolve 1 g of concanavalin A and 0.1 g of acrylic anhydride in 24 mL of phosphate buffer (20 mM, pH 7.4) and react at 51 °C for 2 h, then dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 8 kDa for 7 days. Freeze-dry to obtain allyl concanavalin A monomer.

[0031]Take 75mg of the above-mentioned allyl dextran monomer and 75mg of the above-mentioned allyl concanavalin A monomer, dissolve in 1mL Tris-hydrochloric acid buffer (10mM, pH 8) to obtain a monomer solution; Add 7.5 mg of ammonium persulfate and 3 mg of tetramethylethylenediamine to the so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
degree of substitutionaaaaaaaaaa
degree of substitutionaaaaaaaaaa
Login to view more

Abstract

The invention discloses a new method for preparing an intelligent glucose-sensitive insulin controlled-release biological material. The method comprises the following steps of: firstly, dissolving glucose derivative monomers with C=C derivatives and concanavalin A derivative monomers with C=C double bonds in buffer solution to produce monomer solution; secondly, adding a catalyst in the monomer solution to perform free radical polymerization at a certain temperature; and finally, removing unreacted reactant through dialysis to obtain the glucose-sensitive insulin controlled-release biologicalmaterial. The preparation method has the advantages of convenient implementation, fast reaction process, mild reaction condition and easy production preparation on a large scale. The obtained glucan-concanavalin A insulin controlled-release biological carrier material has the advantages of good glucose-sensitive property and excellent insulin controlled-release performance.

Description

(1) Technical field [0001] The invention relates to a method for preparing a glucose-sensitive biomaterial capable of self-controlling release of insulin, in particular to a new method for preparing a glucose-sensitive dextran-concanavalin A-based insulin self-controllable release biocarrier material, The invention belongs to the technical field of drug controlled release and biomaterials. (2) Background technology [0002] Insulin-dependent diabetes mellitus (type I) is a systemic chronic metabolic system disease, which is very harmful to human health. Controlled release of insulin is an effective method for the treatment of type 1 diabetes. In recent years, the self-regulating plant lectin-saccharide derivative intelligent insulin delivery carrier system developed for the research and development of type I diabetes provides a new way for the treatment of diabetes. The carrier system mainly uses the specific binding properties of plant lectins (such as concanavalin A) and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/36A61K38/28A61P5/48C08B37/02
Inventor 张玮沈绍传
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap