Method for fixing biological molecules on polymer microporous membrane surface

A biomolecules and surface immobilization technology, applied in semi-permeable membrane separation, chemical instruments and methods, membrane technology, etc., can solve the problems of shortened membrane service life, large membrane mass transfer driving force, poor wettability, etc., and achieve equipment Simple, widely applicable, and low-cost effects

Active Publication Date: 2010-06-23
ZHEJIANG UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For most synthetic polymer membranes, the membrane material itself has strong hydrophobicity and poor wettability, which makes it have the following outstanding problems in the aqueous phase separation system: (1) The hydrophobicity of the membrane makes it necessary for water to overcome Higher resistance can pass through the membrane, the driving force of membrane mass transfer is large, and the energy consumption is high; (2) In the organic matter/water separation system (especially the solution containi...

Method used

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  • Method for fixing biological molecules on polymer microporous membrane surface
  • Method for fixing biological molecules on polymer microporous membrane surface
  • Method for fixing biological molecules on polymer microporous membrane surface

Examples

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Embodiment 1

[0052] Dissolve dopa in tris-hydrochloric acid (Tris-HCl) buffer solution to form a dopa solution with a pH value of 7.5 and a concentration of 0.2 g / L; dissolve heparin in 0.01 mol / L phosphoric acid In the buffer solution, prepare heparin solution, the pH value of the solution is 7.4, and the solution concentration is 0.25-2.0 g / L; cut, wash and dry PE flat microporous membrane (prepared by stretching method, average pore size 0.5 micron) 5× 5cm 2 , soaked in 95% ethanol for 1 hour, took it out and placed it in the dopa solution and oscillated for 100 hours, the oscillation frequency was 60 times / min, and the reaction temperature was 10°C to obtain a dopa composite modified PE flat microporous membrane; Soak the dopa composite modified PE flat microporous membrane in the above heparin solution to react for a set time, take out the membrane and place it in deionized water for vibration cleaning, and then vacuum dry. The cleaning vibration frequency is 60 times / min, and the cle...

Embodiment 2

[0057] Dissolve dopamine in tris-hydrochloric acid (Tris-HCl) buffer solution to prepare a dopamine solution with a pH value of 7.5 and a concentration of 0.2 g / L; dissolve heparin in 0.01 mol / L phosphate buffer 20 PP hollow fiber microporous membranes with a length of 10 cm (prepared by stretching method, average pore size of 0.15 microns), washed Clean drying, soaking in 95% ethanol for 1 hour, placing in the above-mentioned dopamine solution, oscillating for 0.5 hours, the oscillation frequency is 80 times / min, and the reaction temperature is 60°C, to obtain a dopamine composite modified PP hollow fiber membrane; Soak the dopamine composite modified PP hollow fiber membrane in the aforementioned heparin solution to react for a set time, take out the membrane and place it in deionized water for oscillation cleaning, the cleaning oscillation frequency is 80 times / min, the cleaning time is 96 hours, and the cleaning temperature is 30°C , and then vacuum-dried at a drying tempe...

Embodiment 3

[0063] Dopa is dissolved in tris-hydrochloric acid (Tris-HCl) buffer solution to prepare a pH value of 10.0 and a concentration of 2.0 grams per liter of dopa solution; BSA is dissolved in 0.01 mol / liter of phosphoric acid In the buffer solution, it is made into BSA solution, the pH value of the solution is 7.4, and the solution concentration is 0.25-2.0 g / L; cut 10 PVDF hollow fiber microporous membranes with a length of 10 cm (prepared by dry-wet spinning method, average pore diameter 0.1 micron), fully washed with deionized water and dried, soaked in industrial ethanol for 1 hour, then placed in the aforementioned dopa solution, oscillating for 24 hours, the oscillating frequency was 70 times / min, and the reaction temperature was 60°C. Obtain the dopa composite modified PVDF hollow fiber membrane; soak the dopa composite modified PVDF hollow fiber membrane in the above-mentioned BSA solution to react until the set time, take out the membrane and place it in deionized water f...

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Abstract

The invention discloses a method for fixing biological molecules on a polymer microporous membrane surface, comprising the following steps: (1) respectively dissolving DOPA compounds and the biological molecules in a trihydroxymethyl aminomethane - muriatic acid buffer solution and a phosphoric acid buffer solution to prepare solutions; (2) immersing a polymer microporous membrane in a DOPA compound solution and oscillating to cause the DOPA compounds to be auto-polymerized-compounded on the membrane surface and to be closely attached to the membrane surface and a membrane pore wall; (3) immersing the polymer microporous membrane modified by the DOPA compounds in a biological molecule solution, and fixing the biological molecules on the polymer microporous membrane surface by the addition reaction between catechol groups of the membrane surface and amidogen in the biological molecules. The method for fixing biological molecules on the polymer microporous membrane surface has simple process, the prepared polymer microporous membrane has good hydrophilic property and biocompatibility, and the method has important meaning for improving the permeability and the biocompatibility of the polymer microporous membrane.

Description

technical field [0001] The invention relates to the technical field of surface modification of polymer microporous membranes, in particular to a method for immobilizing biomolecules on the surface of polymer microporous membranes. Background technique [0002] Membrane technology is a new type of high-efficiency separation technology, which has the advantages of energy saving and environmental friendliness. It is one of the common supporting technologies to solve major problems faced by human beings in the fields of water resources, environment, and energy. It is widely used in water treatment, Chemical separation, food and beverage purification, biomedicine, medical treatment and other fields. Membrane materials are the core of membrane technology. The properties of membrane materials directly affect the physical and chemical stability and separation performance of membranes. The development of modern polymer science has developed polymer membrane materials with different c...

Claims

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Application Information

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IPC IPC(8): B01D71/82B01D67/00
Inventor 朱利平蒋金泓徐又一奚振宇朱宝库
Owner ZHEJIANG UNIV
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