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Adenosine deaminase diagnostic reagent (kit) and adenosine deaminase activity concentration measuring method

A technology of adenosine deaminase and reagents, applied in biochemical equipment and methods, measurement/inspection of microorganisms, measurement of color/spectral characteristics, etc., can solve problems that are difficult to popularize, apply, and measure

Inactive Publication Date: 2010-06-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot be determined by the visible light analyzer in general hospitals, but requires the use of a special ultraviolet light analyzer, so it is difficult to practically popularize and apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The adenosine deaminase diagnostic reagent of this embodiment is a single reagent, including:

[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0043] Stabilizer 500mmol / L

[0044] Coenzyme 3mmol / L

[0045] Nitrite reductase 6000U / L

[0046] Nitrate reductase 8000U / L

[0047] Adenosine 4mmol / L

[0048] Ferricytochrome C 6mmol / L

[0049] Calcium chloride 3mmol / L

[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the tested adenosine deaminase sample to the reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is about 1 minute, the detection time is about 2 minute...

Embodiment 2

[0054] The adenosine deaminase diagnostic reagent of this embodiment is a double reagent, comprising:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Coenzyme 3mmol / L

[0059] Adenosine 4mmol / L

[0060] Ferricytochrome C 6mmol / L

[0061] Calcium chloride 3mmol / L

[0062] Reagent 2

[0063]Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0064] Stabilizer 500mmol / L

[0065] Nitrite reductase 6000U / L

[0066] Nitrate reductase 8000U / L

[0067] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0068] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, test adenosine deaminase sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is p...

Embodiment 3

[0071] The adenosine deaminase diagnostic reagent of this embodiment is three reagents, comprising:

[0072] Reagent 1

[0073] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 50mmol / L

[0075] Coenzyme 3mmol / L

[0076] Adenosine 4mmol / L

[0077] Ferricytochrome C 6mmol / L

[0078] Calcium chloride 3mmol / L

[0079] Reagent 2

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 500mmol / L

[0082] Nitrate reductase 8000U / L

[0083] Reagent 3

[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Nitrite reductase 6000U / L

[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0088] When measuring the activity concentration of adenosine deaminase, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, i...

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Abstract

The invention relates to an adenosine deaminase diagnostic reagent (kit) which utilizes an enzyme colorimetric method and an enzyme linking immunoassay technology, and also relates to an adenosine deaminase activity concentration measuring method as well as composition and components of the reagent, belonging to the technical field of medical test measurement. The reagent (kit) mainly comprises the following components: buffer solution, coenzyme, adenosine, ferricytochrome C, calcium chloride, nitrite reductase, nitrate reductase and stabilizing agent; a sample is mixed with the reagent according to a certain volume proportion, so that a series of enzymatic reaction is carried out; and reactant is arranged under an ultraviolet / visible light analysis instrument, and the absorbance rising speed is detected when the main wavelength is 340nm, so that the activity concentration of the adenosine deaminase can be measured and calculated.

Description

technical field [0001] The invention relates to a diagnostic reagent (box) for adenosine deaminase, and at the same time, the invention also relates to a method for measuring the activity concentration of adenosine deaminase, which belongs to the technical field of medical examination and measurement. Background technique [0002] Medical research shows that adenosine deaminase is a nucleic acid metabolizing enzyme that has an important relationship with the body's cellular immune activity. Therefore, the determination of adenosine deaminase activity is used as an important diagnostic marker for the differential diagnosis of benign and malignant hydrothorax, cerebrospinal fluid, severe combined immunodeficiency disease (SCID), acute and chronic hepatitis, liver cirrhosis, liver cancer and other diseases. [0003] The methods for determining the activity of adenosine deaminase mainly include radionuclide method, physical method and biochemical method. As far as the applicant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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