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Method for measuring total ginkgolic acid in ginkgo biloba extract

A technique for Ginkgo biloba extract and total ginkgolic acid is applied in the field of determination of total ginkgolic acid in Ginkgo biloba extract, which can solve the problems of long extraction and reflux time, large sample sampling amount, low measurement result, etc., and achieves reduction of sampling amount and experimental operation. Convenience, the effect of increasing the speed of dissolving and dispersing

Active Publication Date: 2012-05-23
GUANGZHOU HANFANG PHARMA
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  • Abstract
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  • Application Information

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Problems solved by technology

During this period, standard solutions of several concentrations need to be prepared, and the absorbance is measured to make a standard curve; the sample is pre-purified by silica gel column chromatography; the preparation of ginkgolic acid monomer and the determination of the absorbance coefficient are relatively complicated.
[0009] Due to the lack of systematic research on the extraction and determination of total ginkgolic acids in ginkgo extracts in the existing literature, and the extraction method of analytical sample preparation also has the disadvantages of large sample volume, long extraction and reflux time, and low measurement results, it is necessary to seek a more efficient method. Method for effective determination of total ginkgolic acid in ginkgo extract

Method used

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  • Method for measuring total ginkgolic acid in ginkgo biloba extract

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Experimental program
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Effect test

Embodiment 2

[0035] Embodiment 2: implement the assay scheme of total ginkgolic acid in the ginkgo extract of the present invention:

[0036] Take about 5 g of ginkgo extract powder, weigh it accurately, add 50 ml of 40% methanol solution, that is, the ratio of ginkgo extract to solvent is 1:10, and after ultrasonic treatment for 20 min, the methanol solution is extracted 3 times with 1:1 petroleum ether, Collect the petroleum ether liquid, recover the solvent under reduced pressure to dryness, add methanol to dissolve it, and dilute to 5ml to obtain the test solution. Octadecyl bonded silica gel column, methanol: 1% glacial acetic acid solution = 90:10 as the mobile phase, the detection wavelength is 310nm, and the flow rate is 1ml / min. The reference substance solution and the test solution were injected respectively to obtain a chromatogram. The total ginkgolic acid content was calculated by external standard method. Take 3 samples in parallel, process, and measure total ginkgolic acid...

Embodiment 3

[0037]Embodiment 3: As shown in Embodiment 2, the difference is: add 60% methanol solution, ultrasonic treatment for 20 min, and the methanol solution is extracted 4 times with 1:1 petroleum ether. Take 3 samples in parallel, process, and measure the total ginkgolic acid 620ppm, RSD% is 1.4%. It shows that as the ultrasonic solvent concentration increases to 60% and the number of extractions increases to 4 times, the measured total ginkgolic acid content increases.

Embodiment 4

[0038] Embodiment 4: as shown in Example 2, the difference is: 70% methanol solution, ultrasonic treatment 30min, methanol solution is extracted 4 times with the sherwood oil of 1: 1 amount, get 3 parts of samples in parallel, process, record total ginkgo biloba Acid 677ppm, RSD% is 1.4%. With the increase of ultrasonic time, the content of total ginkgolic acid also increased.

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Abstract

The invention discloses a method for measuring total ginkgolic acid in a ginkgo biloba extract. The method comprises the following steps: the ginkgo biloba extract is dissolved and dispersed by alcohol in an ultrasonic manner, and is extracted by petroleum ether; the petroleum ether is collected; a solvent is recovered until and the extract is dried; methanol is added until the volume is constant, and then the measuring is finished. By adopting the ultrasonic extraction method, the invention can enhance the penetrating power of the solvent against medicinal powder by utilizing the cavitation effect, mechanical effect and thermal effect of ultrasonic waves, so as to increase the dissolving and dispersing speed of the extract, thereby not only being beneficial for uniform dissolving-out anddispersing of the total ginkgolic acid in the ginkgo biloba extract, but also improving the analyzing accuracy for a sticky and caked sample which is poor in physical properties and contains many insoluble components. The invention also has the following characteristics: the sampling quantity is small and the operation is convenient; the sample is not directly dissolved in methanol or petroleum ester but is extracted by petroleum ester, thereby removing partial impurities and protecting columns for HPLC analysis.

Description

technical field [0001] The invention relates to a method for determining total ginkgolic acid in ginkgo extract. Background technique [0002] Ginkgo Biloba is one of the oldest extant seed plants and the only survivor of the Ginkgo family dating back more than 200 million years ago. In China, ginkgo preparations have been used for treatment for more than 5,000 years, that is to say, since the earliest origin of Chinese herbal medicine. Since the 1960s, plant extracts of Ginkgo biloba have been used in the treatment of cerebrovascular and peripheral vascular diseases in many countries, such as Germany, France, Japan and Korea. [0003] The main components of Ginkgo biloba are flavonoids, which include at least 14 different compounds, such as flavonols, flavonoids, flavanones and biflavones, etc. Among these compounds, flavonoid glycosides and flavonol glycosides include kaempferol, quercetin Cortexin and isorhamnetin conjugated with glucose or rhamnose, the most prominent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/16G01N30/02
Inventor 刘翠红钟鸣黄展平全一思蔡鸿飞李金华
Owner GUANGZHOU HANFANG PHARMA