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Homocysteine diagnosis/determination reagent (kit) and homocysteine concentration determination method-982

A homocysteine ​​and reagent technology, applied in the field of medical testing and determination, can solve the problems of long test time, complicated operation, large variation of test data, etc.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Coenzyme 3mmol / L

[0049] Betaine-homocysteine ​​methyltransferase 10000U / L

[0050] Methionine γ-lyase 12000U / L

[0051] Guanosine monophosphate reductase 12000U / L

[0052] Trimethylammonium acetate 7mmol / L

[0053] Inosine monophosphate 7mmol / L

[0054] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0055] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is...

Embodiment 2

[0058] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0059] Reagent 1

[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0061] Stabilizer 50mmol / L

[0062] Coenzyme 3mmol / L

[0063] Trimethylammonium acetate 7mmol / L

[0064] Inosine monophosphate 7mmol / L

[0065] Reagent 2

[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0067] Stabilizer 50mmol / L

[0068] Betaine-homocysteine ​​methyltransferase 10000U / L

[0069] Methionine γ-lyase 12000U / L

[0070] Guanosine monophosphate reductase 12000U / L

[0071] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0072] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, tested homocysteine ​​sample and reagent 1, re...

Embodiment 3

[0075] The homocysteine ​​diagnosis / measurement reagents of this embodiment are three reagents, including:

[0076] Reagent 1

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 50mmol / L

[0079] Coenzyme 3mmol / L

[0080] Trimethylammonium acetate 7mmol / L

[0081] Inosine monophosphate 7mmol / L

[0082] Reagent 2

[0083] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 500mmol / L

[0085] Methionine γ-lyase 12000U / L

[0086] Guanosine monophosphate reductase 12000U / L

[0087] Reagent 3

[0088] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0089] Stabilizer 500mmol / L

[0090] Betaine-homocysteine ​​methyltransferase 10000U / L

[0091] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0092] When measuring the concentration of homocysteine, set it on the automatic biochemic...

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Abstract

The invention relates to a homocysteine diagnosis / determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent compositions and components, which belong to the technical filed of medical test and determination. The reagent (kit) of the invention mainly includes: buffer solution, coenzyme, trimethyl amino acetate salt, inosine monophosphate, betaine-homocysteine methyltransgerase, methionine Gamma-catenase, guanosine monophosphate reductase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet / visible light analyzer to detect the increasing degree of absorbance at the 340nm position of a dominant wave so as to measure and calculate the concentration of the homocysteine.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / determination reagent (kit), and also relates to a method for measuring homocysteine ​​concentration, which belongs to the technical field of medical examination and determination. Background technique [0002] There are many methods for the determination of homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical and enzymatic methods, etc. [0003] 1. High performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. Replaced by fluorescence polarization analysis and enzymatic methods. High-performance liquid chromatography is: first use a reducing agent to convert all...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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