Air bag agitation-type solid-state fermentation method and device thereof

A solid-state fermentation and air bag technology, which is applied in the field of fermentation industry, can solve the problems of difficult amplification and heat transfer, and achieve the effects of wide application range, small shear force, and small mycelium damage

Inactive Publication Date: 2010-07-28
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of reactor does not solve the problem of heat transfer, so it is difficult to scale up; at the same time, this kind of reactor does not have a special device for strengthening gas-liquid transfer, and can only be used for anaerobic fermentation process

Method used

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  • Air bag agitation-type solid-state fermentation method and device thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] Example 1: Production of ethanol by solid-state fermentation of sweet sorghum

[0026] Preparation of materials: remove ears, leaves and roots from the sweet sorghum stalks harvested after maturity, and use a straw crusher to crush them;

[0027] Loading: the pulverized sweet sorghum is loaded into the fermentation tank from the feed port, and the charging coefficient is 50% of the volume of the fermentation tank.

[0028] Sterilization: Pass high-temperature steam through the air inlet (1) of the fermenter to sterilize. The sterilization temperature is 121-125°C, and the sterilization time is 15-20min.

[0029] Inoculation: Mix active dry yeast (Angel Yeast) with sterile water, the mixing ratio is 1g of yeast plus 40mL of sterile water, activate at 35°C for 25-30min, and then place it at room temperature for 45-60min after activation; Add 20ml of activation solution to mix sweet sorghum with activation solution;

[0030] Fermentation: Maintain the ambient temperatur...

example 2

[0032] Example 2: Production of cellulase by solid-state fermentation of steam-exploded wheat straw

[0033] Preparation of materials: The harvested wheat straw is steam-exploded and air-dried for later use.

[0034] Filling: the dry steam-exploded wheat straw and water are mixed and stirred evenly according to the mass ratio of 1:5, and filled into the fermentation tank, and the charging amount is 50% of the tank volume.

[0035] Sterilization: Pass high-temperature steam through the air inlet (1) of the fermenter to sterilize. The sterilization temperature is 121-125°C, and the sterilization time is 15-20min.

[0036] Inoculation: the green wood enzyme (Trichoderma viride) spores are blown into the solid-state fermentation tank with sterile air from the feed port (1) of the tank body, and the inoculum amount is 1g of green wood enzyme spores per tank.

[0037] Fermentation: Maintain the ambient temperature of the fermenter at about 30°C for fermentation. Blow in sterile a...

example 3

[0039] Example 3: Production of alkaline protease for steam-exploded wheat straw solid-state fermentation

[0040] Preparation of materials: Cut the cleaned polyurethane foam into square particles with a side length of 1 cm for later use. In addition, a liquid medium was prepared, and the ingredients were peptone 10g / L, beef extract 0.5g / L, and NaCl 0.5g / L.

[0041] Filling: Fill the above-mentioned dry polyurethane foam into the fermentation tank, and the charging amount is 50% of the tank volume.

[0042] Sterilization: Pass high-temperature steam through the air inlet (1) of the fermenter to sterilize. The sterilization temperature is 121-125°C, and the sterilization time is 15-20min. The medium was sterilized in a separate container at the same temperature and time.

[0043] Inoculation: Mix the suspension of Bacillus pumilus with a concentration of about 106 / ml with the sterilized and cooled medium at a volume ratio of 1:10, and spray it into the fermenter from the noz...

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Abstract

The invention discloses an air bag agitation-type solid-state fermentation method and a device thereof. The air bag type solid-state fermentation method disclosed by the invention is characterized in that a closed tank body is internally provided with an air bag which can inflate and deflate, materials are pushed to reciprocate in the tank body through the form changes caused by the inflation andthe deflation of the air bag, and at the same time, the interphase transmission process is strengthened through the changes of pressure in tank body, which is caused by the expansion and contraction of the air bag. The method strengthens the mass transport process of the solid-state fermentation through the gentle stirring and normal force, at the same time, avoids the hurt of shear force in the operation of a general dynamic solid-state fermentation reactor to microorganism. The invention not only can be used for the aerobic solid state fermentation process but also can be used for an anaerobic fermentation solid-state fermentation system, has simple structure and wide application range, and provides a new technical platform for the fermentation industry.

Description

technical field [0001] The invention belongs to the technical field of fermentation industry Background technique [0002] Solid-state fermentation is a biological reaction process carried out on a solid substrate that does not contain or contains a small amount of free water. Because solid-state fermentation has the characteristics of low energy consumption, simple process, ability to use solid substrates and less water consumption, solid-state fermentation plays an important role in the production of food, enzymes, biopesticides and other products. [0003] In recent years, due to the efforts of scientists and engineers, solid-state fermentation has moved from extensive traditional production methods to modern solid-state fermentation characterized by large-scale and pure strains (or combined strains). This has made great progress in the research and application of solid-state fermentation. However, due to the complexity and variety of solid-state fermentation systems, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02
CPCC12M21/16C12M41/40C12M27/00C12M27/02
Inventor 陈洪章张志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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