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Polypeptide with inhibiting virus mixed infection activity

A virus and active ingredient technology, applied in the direction of antiviral agents, medical preparations containing active ingredients, peptides, etc., can solve the problems of complicated epidemic situation, difficult diagnosis and prevention of livestock and poultry diseases, etc., and achieve good stability and inhibitory ability strong effect

Inactive Publication Date: 2010-08-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These new advances in human virology undoubtedly provide us with a lot of inspiration and ideas, but unfortunately, the research is highly concentrated on anti-HIV virus inhibitors, how to develop high-efficiency agents that can resist mixed virus infections in aquaculture on this basis, new challenges and opportunities
[0004] The three diseases caused by Newcastle disease virus, Marek virus and infectious bronchitis virus, which seriously threaten the poultry industry, usually occur in the form of mixed infection. The mixed infection of viruses complicates the epidemic situation and causes many difficulties in the diagnosis and prevention of livestock and poultry diseases

Method used

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  • Polypeptide with inhibiting virus mixed infection activity
  • Polypeptide with inhibiting virus mixed infection activity
  • Polypeptide with inhibiting virus mixed infection activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1. Preparation and functional testing of novel polypeptides

[0074] 1. Preparation of new peptides

[0075] Gene redesign and splicing were carried out on the basis of the sequence comparison of the HR regions of the two viruses (NDV and IBV), that is, according to the inherent characteristics of the HR region—the a site is highly conserved (mostly leucine I / isoleucine L), the non-important helix-forming unit is replaced by lys / glutamic acid without affecting the structure-in short, the designed HR polypeptide will only need to consider the amino acid properties of the d and e sites in the HR regions of two different viruses different( figure 1 ).

[0076] PGEX-6p-1 vector (purchased from Amersia Company, catalog number is P0048); BL21(DE3) bacteria (purchased from Beijing Kangwei Century Company, catalog number is CW0809).

[0077] 1. The amino acid sequence of the N1 polypeptide is: NASDMEIKKVNKKIEEYIKKIEEVEKKLEEVNKK (sequence 1 in the sequence listing). ...

Embodiment 2

[0182] Embodiment 2, preparation and functional detection of control polypeptide

[0183] 1. Antiviral activity of HR2 polypeptide of IBV, HR2 polypeptide of NDV and HR2 polypeptide of MDV

[0184] (1) Preparation of each polypeptide

[0185] 1. Gene cloning, protein expression and purification: Respectively digest and recover the following PCR products and clone them into the PGEX-6p-1 vector (purchased from Amersia Company, catalog number P0048), and transform the positive clone into BL21 (DE3 ) bacteria (purchased from Beijing Kangwei Century Company, the product catalog number is CW0809) for protein expression, the supernatant was obtained by centrifugation after ultrasonic cracking of the expressed bacteria, and the supernatant was passed through a Glutathione-Sepharose 4B affinity chromatography column equilibrated with PBS ( (purchased from Oriental Science and Technology Company), and then wash the affinity column with PBS for at least 10 column volumes, followed by a...

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Abstract

The invention discloses polypeptide with inhibiting virus mixed infection activity. The polypeptide is polypeptide shown as the following 1) or 2): 1) the polypeptide of which the amino acid sequence is shown in a sequence 1 in a sequence table; and 2) the polypeptide of which the amino acid sequence is shown in a sequence 2 in the sequence table. The polypeptide provides theoretical basis for developing high-efficiency novel micromolecular oral medicaments for resisting virus mixed infection, provides a new strategy for preventing and controlling viral diseases of bred livestock and poultry and economic animals, and has important application prospect and innovation significance in the field of animal medical science.

Description

technical field [0001] The invention relates to a polypeptide having the activity of inhibiting virus mixed infection. Background technique [0002] It is a very complicated process for enveloped viruses to invade host cells. According to the properties and structural changes of their envelope fusion glycoproteins, retroviruses, paramyxoviruses (such as Newcastle disease virus, NDV), coronaviruses ( Such as infectious bronchitis virus, Infectious bronchitis virus, IBV), orthomyxovirus and filovirus fusion protein is classified as type I enveloped virus glycoprotein, which can be seen under the electron microscope with obvious "fibrils" or spikes; Flaviviruses and togavirus fusion proteins are classified as type II enveloped virus glycoproteins, which do not form "fibrils" and are rich in β-sheet structures; recent studies have shown that herpes viruses (such as Marek's disease virus, MDV), The fusion glycoproteins of rhabdoviruses and baculoviruses have some characteristics...

Claims

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Application Information

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IPC IPC(8): C07K14/00C12N15/11C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A61K38/16A61P31/12
Inventor 王晓佳朱德兵汪明
Owner CHINA AGRI UNIV
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