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Issatchenkia occidentalis strain and preparation method thereof and method for preparing xylitol and ethanol with strain

A technology of Isaac yeast and xylitol, applied in the field of detoxification active microbial strains, can solve problems such as high cost and cumbersome process

Inactive Publication Date: 2010-08-18
唐传生物科技(厦门)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The purpose of the present invention is to solve the problems of cumbersome process and high cost in the existing hemicellulose hydrolysis physical chemical detoxification process

Method used

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  • Issatchenkia occidentalis strain and preparation method thereof and method for preparing xylitol and ethanol with strain
  • Issatchenkia occidentalis strain and preparation method thereof and method for preparing xylitol and ethanol with strain
  • Issatchenkia occidentalis strain and preparation method thereof and method for preparing xylitol and ethanol with strain

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Experimental program
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Embodiment Construction

[0079] operation 1

[0080] Weigh 3 kg of bagasse that has been washed and re-dried, add H2SO4 solution (2.5%, w / w) at a solid-to-liquid ratio (w / v) of 1:7, hydrolyze at 120 ° C for 2 h, collect the filtrate by centrifugation, wash the filter residue once, and combine The filtrate was adjusted to pH 3 with solid calcium carbonate, and the precipitate was removed by suction filtration to obtain bagasse hemicellulose hydrolyzate. The reducing sugar content of the hydrolyzate is 3%, and xylose monosaccharide accounts for more than 80% of the total sugar.

[0081] operation 2

[0082] Take 2 kg of crushed dry corncobs, add 2% (w / w) H at a solid-to-liquid ratio of 1:7 2 SO 4 The solution was hydrolyzed at 120°C for 2 hours, the liquid part was collected, neutralized with calcium carbonate to a pH value of 3, and the precipitate was removed by suction filtration to obtain a dilute acid hydrolyzate of corncob hemicellulose. The total sugar content of the hydrolyzate is 5%, wherei...

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Abstract

The invention relates to a microbiological strain Issatchenkia occidentalis (Lj-3) (CCTCC NO:M206097). The nucleotide characteristic sequence in the 26SrDNA D1 / D2 region of the strain has 100% homology with the characteristic sequence with registration number in GenBank being EF030708. The invention also discloses a preparation method and biological detoxication application of the strain. The preparation method is characterized by mixing and inoculating the strain and the microorganisms for producing xylitol by fermenting xylose to realize synchronous detoxication and fermentation of hemicellulose hydrolysates to produce xylitol; and mixing and inoculating the detoxified active strain and the microorganisms for producing ethanol by fermenting xylose to realize synchronous detoxication and fermentation of the hemicellulose hydrolysates to produce ethanol. Compared with the traditional art, the production efficiency and the product quality of the invention are higher.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for biological detoxification of hemifiber hydrolyzate and special detoxification active microbial strains. Background technique [0002] Hemicellulose is a polysaccharide other than cellulose in the plant cell wall. The content varies with different plants, accounting for 20-35% of the weight of the plant. It is the most abundant polysaccharide on the earth except cellulose. [0003] Cellulose is a homogeneous polysaccharide composed entirely of glucose units, while hemicellulose is mainly composed of xylose with β-(1-4) glycosidic bonds to form the main chain, and arabinose, mannose and galactose form branches. Non-uniform glycans with a chain structure. The xylan backbone of hemicellulose generally contains 50-150 xylose units, has no crystalline structure, and is bound to the surface of cellulose microfibrils. [0004] Hemicellulose is much easier to hydrolyze th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P19/02C12P7/10C12R1/74C12R1/645
CPCY02E50/16Y02E50/10
Inventor 张厚瑞覃香香蔡爱华周玉恒陈海珊
Owner 唐传生物科技(厦门)有限公司
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