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Application of Issatchenkia occidentalis in biological detoxication and method of utilizing Issatchenkia occidentalis to synchronously detoxify and ferment hemicellulose hydrolysates

A technology of hemicellulose and Isaac yeast, applied in the biological field, can solve the problems of high cost and cumbersome process

Inactive Publication Date: 2010-08-18
唐传生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to solve the problems of cumbersome process and high cost in the existing hemicellulose hydrolysis physical chemical detoxification process

Method used

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  • Application of Issatchenkia occidentalis in biological detoxication and method of utilizing Issatchenkia occidentalis to synchronously detoxify and ferment hemicellulose hydrolysates
  • Application of Issatchenkia occidentalis in biological detoxication and method of utilizing Issatchenkia occidentalis to synchronously detoxify and ferment hemicellulose hydrolysates
  • Application of Issatchenkia occidentalis in biological detoxication and method of utilizing Issatchenkia occidentalis to synchronously detoxify and ferment hemicellulose hydrolysates

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Experimental program
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Embodiment Construction

[0079] operation 1

[0080] Weigh 3 kg of bagasse that has been washed and re-dried, add H2SO4 solution (2.5%, w / w) at a solid-to-liquid ratio (w / v) of 1:7, hydrolyze at 120 ° C for 2 h, collect the filtrate by centrifugation, wash the filter residue once, and combine The filtrate was adjusted to pH 3 with solid calcium carbonate, and the precipitate was removed by suction filtration to obtain bagasse hemicellulose hydrolyzate. The reducing sugar content of the hydrolyzate is 3%, and xylose monosaccharide accounts for more than 80% of the total sugar.

[0081] operation 2

[0082] Take 2 kg of crushed dry corncobs, add 2% (w / w) H at a solid-to-liquid ratio of 1:7 2 SO 4 The solution was hydrolyzed at 120°C for 2 hours, the liquid part was collected, neutralized with calcium carbonate to a pH value of 3, and the precipitate was removed by suction filtration to obtain a dilute acid hydrolyzate of corncob hemicellulose. The total sugar content of the hydrolyzate is 5%, wherei...

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Abstract

The invention relates to a microbiological strain Issatchenkia occidentalis (Lj-3) (CCTCC NO:M206097) and application of a domesticated strain thereof. The invention is characterized in that the pure culture of Issatchenkia occidentalis (Lj-3) is inoculated to the hemicellulose hydrolysates and is cultured so as to be used for degrading the microbial metabolism inhibitors in the hemicellulose hydrolysates and further for realizing biological detoxication of the hemicellulose hydrolysates. The invention also discloses a method for preparing xylitol and ethanol with the strain. Compared with the traditional art, the production efficiency and the product quality of the invention are higher.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a biological detoxification application of Isakula westernis and a method for synchronous detoxification and fermentation of hemicellulose hydrolyzate. Background technique [0002] Hemicellulose is a polysaccharide other than cellulose in the plant cell wall. The content varies with different plants, accounting for 20-35% of the weight of the plant. It is the most abundant polysaccharide on the earth except cellulose. [0003] Cellulose is a homogeneous polysaccharide composed entirely of glucose units, while hemicellulose is mainly composed of xylose with β-(1-4) glycosidic bonds to form the main chain, and arabinose, mannose and galactose form branches. Non-uniform glycans with a chain structure. The xylan backbone of hemicellulose generally contains 50-150 xylose units, has no crystalline structure, and is bound to the surface of cellulose microfibrils. [0004] Hemicellulose is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P19/02C12P7/10C12R1/74C12R1/645
CPCY02E50/16Y02E50/10
Inventor 张厚瑞覃香香蔡爱华周玉恒陈海珊
Owner 唐传生物科技(厦门)有限公司
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