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Composite microbial preparation for degrading aflatoxin

A technology of compound microbial inoculum and aflatoxin, applied in the field of microorganisms, can solve the problems of nutrient deficiency, increased cost, vitamin and mineral adsorption, etc., and achieve the effects of increasing nutrients, changing odor, and reducing mycotoxins

Inactive Publication Date: 2010-08-25
沈阳科丰牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Existing research on mold removal is aimed at the screening of some strains, which can be applied in practice in microorganisms. At present, the method of irradiation is used. This method is to use electron beams to irradiate samples containing aflatoxin to obtain aflatoxins. For samples with reduced aspergillus toxin content, although the method of irradiation is simple, the bacteria will also release endotoxin during irradiation and sterilization, which will bring other unfavorable factors. There are also some mold removers that can cause vitamins and minerals to The adsorption of the fungicide will lead to nutrient deficiency. If the mold remover is added, the amount of micronutrients used will be increased, which will increase the cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0010] The preparation method of the composite microbial bacterial agent: count the Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis in parts by weight after the propagation and cultivation: Enterococcus faecalis: Lactobacillus acidophilus : Bacillus subtilis == 1: 1: 1: 1, and then cultivated in the culture medium for 60 hours at 28-30°C to form a composite microbial agent, the culture medium being 1g of agar, 1g of beef extract, yeast extract powder 2.5g, 35g sucrose, 1.5g defoamer, 1g peptone, add distilled water to 1000ml, pH 6.0, autoclave at 121°C for 20 minutes, set aside. The dosage form of the compound microbial bacterial agent is a spray type.

[0011] The Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis were proliferated and cultured in the following respective media according to conventional methods.

[0012] The Candida utilis was cultured in 3 g of potato dipping powder (commercially ava...

Embodiment 2

[0021] Composite microbial bacterial agent: the Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis after the proliferation culture are calculated in parts by weight. Candida utilis: Enterococcus faecalis: Lactobacillus acidophilus: Bacillus subtilis Bacteria == 1:2:2:1, and then cultivated in the medium for 72 hours at 30-32°C to form a compound microbial agent. Application example: apply the above-mentioned spray-type compound microbial agent to moldy feed, a total of 50kg , the above-mentioned composite microbial bacterial agent is 500ml, according to natural air-drying, can measure aflatoxin content (referring to Table 2).

[0022] Table 2 The average result of the detection of the aflatoxin content of the compound microbial preparation at different times (μg / kg)

[0023]

[0024] It can be seen from the table that the composite microbial spray has the effect of degrading aflatoxin, and it can be reduced to 66.40% in 24 hours. A...

Embodiment 3

[0026] Composite microbial bacterial agent: the Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis after the proliferation culture are calculated in parts by weight. Candida utilis: Enterococcus faecalis: Lactobacillus acidophilus: Bacillus subtilis Bacteria == 3:1:1:1, and then cultivated in the culture medium at 35-37°C for 65 hours, which is the composite microbial bacterial agent.

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PUM

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Abstract

The invention relates to the field of microbe, in particular to a composite microbial preparation for degrading aflatoxin. The composite microbial preparation consists of candida utilis, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis which are cultured in a culture medium for 60 to 72 hours at the temperature of between 28 and 37 DEG C, wherein the preparation consists of 1 to 3 weight parts of candida utilis, 1 to 3 weight parts of enterococcus faecalis, 1 to 3 weight parts of lactobacillus acidophilus and1 to 3 weight parts of bacillus subtilis.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a composite microbial bacterial agent for degrading aflatoxin. Background technique [0002] Existing research on mold removal is aimed at the screening of some strains, which can be applied in practice in microorganisms. At present, the method of irradiation is used. This method is to use electron beams to irradiate samples containing aflatoxin to obtain aflatoxins. For samples with reduced aspergillus toxin content, although the method of irradiation is simple, the bacteria will also release endotoxin during irradiation and sterilization, which will bring other unfavorable factors. There are also some mold removers that can cause vitamins and minerals to The adsorption of the fungicide will lead to nutrient deficiency. If the mold remover is added, the amount of micronutrients used will be increased, which will increase the cost. Contents of the invention [0003] The purpose o...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/16A01N63/02A01N63/04A01P3/00A62D3/02C12R1/72C12R1/01C12R1/23C12R1/125A62D101/20
Inventor 高林白子金朱立良张艳平徐斌龙张力邢迪黄鹤陈思赵明侯建华张玥罗桂扬吕良王福喜
Owner 沈阳科丰牧业科技有限公司