Method for preparing animal model with cardiac hypertrophy by unartificial ventilation

A technique of myocardial hypertrophy and animal model, which is applied in the fields of drug devices, other medical devices, animal husbandry, etc., can solve the problems of increasing experimental conditions and operation links, tracheal injury, complications of mechanical ventilation, etc., and achieves good modeling effect. The effect of the same degree of narrowing and simple preparation method

Inactive Publication Date: 2010-09-15
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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Problems solved by technology

It is generally believed that a more ideal pressure overload left ventricular hypertrophy model can be established by constriction of the proximal ascending aorta. Recently, there have been reports of related models abroad, but these studies often require tracheal intubation and ventilator assistance for experimental animals. Breathing (this method is called artificial ventilation), which increases the experimental conditions and operating links, and more importantly, may cause serious tracheal damage and complications of mechanical ventilation

Method used

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  • Method for preparing animal model with cardiac hypertrophy by unartificial ventilation
  • Method for preparing animal model with cardiac hypertrophy by unartificial ventilation
  • Method for preparing animal model with cardiac hypertrophy by unartificial ventilation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Establishment of rabbit aortic valve ascending aortic coarctation model

[0019] Healthy New Zealand white rabbits, male or female, weighing 1.94±0.11kg, were provided by the Experimental Animal Center of Xinqiao Hospital Affiliated to Third Military Medical University. They were randomly divided into two groups, ascending aortic coarctation group (AB group) and sham operation group (sham group).

[0020] Rabbits were anesthetized by intravenous injection of 3% pentobarbital sodium solution (30mg / kg), without endotracheal intubation and ventilator-assisted ventilation, keeping the rabbits in a natural breathing state, fixed on the operating table in supine position, without oxygen. In order to prevent the occurrence of pneumothorax, cut the sternum longitudinally from the xiphoid process strictly along the center of the sternum to the jugular notch, and use a children's chest opener to stretch the sternum. Before cutting the pericardium, do not stretch excessi...

Embodiment 2

[0021] Example 2 Ultrasonic Doppler Detection

[0022] Echocardiography detected the flow velocity at the constriction, estimated the pressure difference, and verified the effect of aortic constriction. The results are shown in Figure 1. The blood flow velocity at the constriction increased significantly in the AB group.

Embodiment 3

[0023] Example 3 Left Ventricle Myocardium Routine Pathological Slice Examination

[0024] Formaldehyde-fixed, paraffin-embedded, HE stained. The characteristics of pathological changes of myocardial fibrosis after pressure overload and the pathological outcome of myocardial fibrosis after complete pressure overload were examined by routine pathological sections.

[0025] The results are shown in Figure 2. The arrangement of myocardial fibers in the sham operation group was normal, without vasodilation and vascular wall thickening. In the ascending aorta coarctation group, the arrangement of myocardial fibers was disordered, the cardiomyocytes were hypertrophic, the branches increased, and interstitial collagen fibers proliferated significantly.

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Abstract

The invention relates to a method for preparing an animal model with cardiac hypertrophy by unartificial ventilation, comprising the following steps of: (1) narcotizing a healthy rabbit without using a breathing machine and keeping the natural breathing state of the rabbit; (2) ligaturing the root of an ascending aorta by using a Prolenen thread with the model of 3 / 0 so that the diameter of the ascending aorta is reduced by 45-55 percent; and (3) obtaining a model animal with obvious cardiac hypertrophy and coarctated ascending aorta after 4-8 weeks. The animal model is prepared without the breathing machine, thereby avoiding tracheal injury and complicating diseases of mechanical ventilation, reducing experimental facilities, lowering the cost, and playing a very important role in researching the mechanism of cardiac hypertrophy and treating.

Description

technical field [0001] The invention relates to a method for establishing an animal model, in particular to a method for preparing an animal model of cardiac hypertrophy without artificial ventilation. Background technique [0002] Myocardial hypertrophy is more common in diseases with long-term increase in pressure load, such as hypertension, aortic stenosis, etc. increase etc. This compensatory response is often beneficial in the early stage, and can maintain normal ventricular wall tension and oxygen consumption, but long-term left ventricular hypertrophy will significantly increase the incidence of heart failure and sudden death. At present, the molecular mechanism of cardiac hypertrophy is still unclear. [0003] The establishment of animal models plays a very important role in the study of the mechanism and treatment of myocardial hypertrophy. At present, the animal models of myocardial hypertrophy with pressure overload are mostly produced by spontaneous hypertensio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A01K67/027A61M31/00
Inventor 赵哲陈林肖颖彬郝佳张载高贝亚军解水本李寒迟海涛潘绪郑德志
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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