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Rice protein OsAHL and use thereof

A technology of rice and protein, applied in the field of genetic engineering, can solve problems such as specific functions that have not yet been involved in research

Inactive Publication Date: 2010-09-15
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The DUF296 functional domain is a kind of protein sequence and structure conserved in prokaryotes and plants, and its specific function has not been studied yet

Method used

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  • Rice protein OsAHL and use thereof
  • Rice protein OsAHL and use thereof
  • Rice protein OsAHL and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Analysis of the expression profile of rice gene OsAHL in rice variety IRAT109

[0061] 1. Coercion treatment

[0062] (1) Drought treatment: After germination of IRAT109 seeds, they were transplanted into soil for cultivation, and drought treatment was carried out at the seedling stage (4 leaves, one new), the tillering stage (6 leaves, one new), and the booting stage (meiosis stage). In each period, when the soil layer in the pot was dry, the water was cut off. At the same time, as the beginning of the drought treatment, the leaves of the plants were cut at the same time every day during the drought treatment period, and they were frozen in liquid nitrogen for RNA extraction.

[0063] (2) Treatment with hormones and chemical reagents at the seedling stage: After the seeds of IRAT109 were accelerated to germination, they were grown in hydroponics in the germination box. After the three-leaf stage, a nutrient solution (standard nutrient solution of the Interna...

Embodiment 2

[0071] Cloning of embodiment 2 rice OsAHL gene

[0072] 1. Seedling cultivation

[0073] Rice (variety "IRAT109") was collected and preserved by the Germplasm Resource Bank of Shanghai Agricultural Biological Gene Center. The rice was germinated at 35°C for 48 hours, and then sown in the greenhouse. When the rice leaves were 3-5 pieces, it was ready to be extracted. DNA or RNA.

[0074] 2. Extraction of RNA: RNA was extracted according to the method in Example 1.

[0075] 3. Full-length cloning of genes

[0076] According to the sequence information provided in the rice database information in GeneBank, primers were designed, and the full-length cDNA was cloned by RT-PCR method.

[0077] A cDNA sequence (SEQ ID NO.2) containing a complete open reading frame (SEQ ID NO.2) was obtained by RT-PCR [Pf1 (SEQ ID NO.3)+Pr1 (SEQ ID NO.4)], and the length was 1331bp; recovery, Connect to the pGEMT-Easy vector, use SP6 or T7 as a universal primer, and use the method of terminator fl...

Embodiment 3

[0104] Example 3 Sequence information and homology analysis of rice OsAHL gene

[0105] The rice OsAHL full-length cDNA of the present invention has a length of 1331bp, and its sequence is shown in SEQ ID NO.2, wherein the open reading frame is located at 69-1169 nucleotides (1101 nucleotides). The amino acid sequence of rice OsAHL was deduced according to the full-length cDNA, with a total of 366 amino acid residues, a molecular weight of 37.2k Daltons, and an isoelectric point (pI) of 7.57. The sequence is shown in SEQ ID NO.1.

[0106] The full-length cDNA sequence of rice OsAHL and its encoded protein were used to perform nucleotide and protein homology in Non-redundant GenBank+EMBL+DDBJ+PDB and Non-redundant GenBank CDS translations+PDB+SwissProt+Superdate+PIR databases using BLAST program The results of the sex search showed that the OsAHL gene was 100% homologous to the Os11g0149100 gene at the nucleotide and amino acid levels. Neither the Os11g0149100 gene nor its ass...

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Abstract

The invention provides a protein OsAHL associated with rice stress resistance (including but not limited to drought resistance, low-temperature resistance and high salt stress resistance) and chloroplast growth and a nucleotide sequence for coding polypeptide having the activity of the rice protein OsAHL. 70 percent of the nucleotide sequence is homologous with a nucleotide sequence from position 69 to position 1169 in a nucleotide sequence SEQ ID No.2, so the nucleotide sequence can be crossed with the nucleotide sequence from position 69 to position 1169 in the nucleotide sequence SEQ ID No.2. The rice gene OsAHL provided by the invention has the functions of regulating the growth of chloroplasts and improving the activity and stability of a photosynthetical system; the gene plays an obvious role in the aspect of plant stress resistance (including but not limited to drought resistance, low-temperature resistance and high salt stress resistance) and therefore has a high application value; and the gen can obviously reduce the loss of the yield and biological yield of crops under the drought, semi-arid, high-salt content and low-temperature conditions or other conditions.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to rice OsAHL protein and its application. Background technique [0002] Rice is the most water-consuming crop in agricultural production, and its water-saving and drought-resistant properties are of great significance to my country's food, water and ecological security. Molecular breeding using genetic engineering technology to transfer drought-resistant genes into excellent rice varieties, thereby improving their drought resistance, and cultivating new varieties of crops that are drought-resistant, high-yielding and high-quality is one of the effective ways of drought-resistant breeding. [0003] Discovering drought-resistant genes is the basis of drought-resistant molecular breeding. There are more and more published studies on drought resistance-related genes and their proteins. Trans-transcriptional regulators in a broad sense include all protein molecules that regulate gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/63C12N5/10C12N1/19A01H5/00
Inventor 周立国刘灶长刘运华孔德艳秦建英罗利军
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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