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Method for preparing gardenia red pigment by using immobilized enzyme

A technology of immobilizing enzymes and gardenia red, applied in the directions of immobilization on/in organic carriers, fermentation, etc., can solve the problems of difficult separation and recovery of enzyme liquid, complicated purification and enrichment process, low product color and price, etc. The effect of saving resources, low price and simple production process

Inactive Publication Date: 2010-09-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a method for preparing gardenia red pigment by using geniposide, a by-product of yellow pigment extracted from gardenia, as raw material, through alkali hydrolysis, immobilized enzyme enzymolysis, and acid precipitation enrichment, which can solve the problem of traditional In the process, the enzyme liquid is not easy to separate and recover, the purification and enrichment process is complicated, the cost is high, and the product color price is low, and the resource utilization rate can be improved

Method used

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  • Method for preparing gardenia red pigment by using immobilized enzyme
  • Method for preparing gardenia red pigment by using immobilized enzyme

Examples

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Effect test

Embodiment 1

[0053] (1) Preparation of immobilized enzyme

[0054] Mix 30g of sodium alginate with 20g of gelatin, add it to 1L of water, stir to dissolve at 40℃, add 40g of cellulase, stir and mix well, shake at 40℃ constant temperature shaker, 100r / min for 0.5h, Then the mixture was poured dropwise into 1.5mol / L calcium chloride solution, and allowed to stand for 60min at 4℃ to make it agglomerate and solidify, collect the gel pellets, and use 0.2mol / L sodium chloride solution Washing, suction filtration to remove free water, collecting gel pellets to obtain immobilized enzyme.

[0055] (2) Preparation of gardenia red pigment

[0056] Weigh 0.5Kg of geniposide powder and dissolve it in 3L of sodium hydroxide solution with a concentration of 4mol / L, and react on a shaker at 55°C for 8 hours. After the reaction is over, adjust the reaction solution with 5mol / L citric acid solution to pH4.5, add immobilized cellulase with a wet weight of 100g, hydrolyze at 50°C for 12 hours, filter, recover th...

Embodiment 2

[0058] (1) Preparation of immobilized enzyme

[0059] Mix 4g of sodium alginate solution with 2g of modified starch, add to 100ml of water, stir to dissolve at 40°C, add 2g of β-glucosidase, stir and mix well, place it on a constant temperature shaker at 37°C, 50r / Min shake for 1h, then inject the mixture dropwise into a 2.0mol / L calcium chloride solution, and let it stand for 120min at 4℃ to make it agglomerate and solidify. Collect the gel pellets and use 0.2mol / L Wash with sodium chloride solution, remove free water by suction filtration, collect gel pellets to obtain immobilized enzyme.

[0060] (2) Preparation of gardenia red pigment

[0061] Weigh 15g of geniposide powder and dissolve it in 90ml of 3.5mol / L sodium hydroxide solution, and react on a shaker at 50°C for 12 hours. After the reaction, adjust the reaction solution to pH4.5, add immobilized β-glucosidase with a wet weight of 5g, enzymolyze at 50℃ for 8 hours, filter, recover the immobilized enzyme, add 8g sodium ...

Embodiment 3

[0063] (1) Preparation of immobilized enzyme

[0064] Add 100g of sodium alginate to 8L of water, stir to dissolve at 38°C, add 50g of pectinase, stir and mix well, shake on a constant temperature shaker at 37°C at 80r / min for 0.6h, and inject the mixture dropwise In a 2.0mol / L calcium chloride solution, let stand for 100min at 3℃ to make it agglomerate and solidify, collect the gel pellets, wash with 0.15mol / L sodium chloride solution, remove free water by suction and collect The gel beads obtain immobilized enzymes.

[0065] (2) Preparation of gardenia red pigment

[0066] Weigh 1Kg of geniposide powder and dissolve it in 4L of sodium carbonate solution with a concentration of 5mol / L, and react on a shaker at 60℃ for 12 hours. After the reaction, adjust the reaction solution to pH 4.7 with 5mol / L acetic acid solution Add the immobilized pectinase with a wet weight of 150g, hydrolyze the enzyme at 50°C for 12 hours and filter, recover the immobilized enzyme, add 0.8Kg of glycine...

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Abstract

The invention provides a method for preparing a gardenia red pigment by using an immobilized enzyme, which comprises the following steps of: using geniposide as a raw material; performing alkaline hydrolysis and acidification on the raw material, and adding the immobilized enzyme into the raw material to perform enzymolysis; filtering the mixture to obtain enzymolysis solution; adding a primary amino compound into the enzymolysis into to perform a reaction; filtering the mixture to obtain solution of the gardenia red pigment; and performing acid deposition, deposition washing neutralization, dehydration and drying on the solution of the gardenia red pigment to obtain the gardenia red pigment. The method has the advantages that: an enzyme preparation can be recycled, the reaction condition is mild, an acid deposition method is adopted to perform enrichment, the process is simple, the energy consumption is low, and a product can be separated easily. The gardenia red pigment prepared by the method can obviously reduce the production cost. The product has the maximum absorption of 530 nm, has high water-solubility and high light, heat, acid, alkali and metal ion stability, and is favorable for widening the application range of the gardenia red pigment.

Description

Technical field [0001] The invention relates to a method for preparing gardenia red pigment by using an immobilized enzyme, in particular a method for preparing gardenia red pigment by using geniposide as a raw material through alkaline hydrolysis, immobilized enzyme hydrolysis, and acid precipitation enrichment. Background technique [0002] With the development of the food industry and the increasing improvement of people's living standards, food pigments as an important part of food additives have received more and more attention. Gardenia ( GardeniaJasminoide Eills) are widely distributed in southern my country. It is an ornamental medicinal plant that can be cultivated artificially and has abundant resources. Gardenia red pigment is a deep-processed product of Gardenia, with high safety, good stability, and good development and application prospects. [0003] The invention patent ZL200610038494.8 of the People’s Republic of China discloses a purification method for the produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/12C12N11/04
Inventor 甘纯玑周庆新谢苗
Owner FUJIAN AGRI & FORESTRY UNIV
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