Method for performing rapid gene typing on trace human papilloma virus (HPV)

Abstract

The invention discloses a method for performing rapid gene typing on trace human papilloma virus (HPV), which has the characteristics of wide application range, high sensitivity, accuracy and high efficiency. Based on a polymerase chain reaction (PCR) detection method, nested amplification is performed on an HPV L1 conservative region for three times, electrophoresis detection and sequencing are performed on a target product and the target product is typed by using special analysis software so as to correctly and rapidly diagnose trace HPV virus infection and detect and identify various HPV gene types, such as high-risk type, low-risk type and the like. The method has high sensitivity and wide application range, can be used for detecting various types of samples, such as paraffin sections, formalin-dipped tissues, blood samples, cast-off cells and the like, and is convenient to apply to early screening of cervical cancer.

Description

technical field [0001] The invention relates to a rapid genotyping method for trace HPV. Background technique [0002] Cervical cancer is the malignant tumor with the highest morbidity and mortality rate in the female reproductive tract. About hundreds of thousands of women die from cervical cancer in the world every year. It is the most serious disease that threatens women's lives. The occurrence and development of cervical cancer is a process from gradual change to mutation. Planned screening is very critical for effective prevention and early treatment of cervical cancer and its precancerous lesions. It is of great social significance to promote the application of advanced detection technology for early diagnosis and treatment of cervical cancer and precancerous lesions and to protect the reproductive health of women. [0003] Clinical scientific research shows that persistent infection of human papillomavirus (HPV) is the direct cause of cervical cancer. HPV can be div...

AI Technical Summary

Problems solved by technology

[0005] At present, the detection methods of HPV mainly include: PCR detection, the most commonly used is MY09 / 11, GP05 / 06 primer amplification, and then combined with ELESA agent PCR reverse dot hybridization, but the follow-up work is complicated and requires high experimental technology; combined target Automatic gene detection, rapid typing of HPV by using gene chip and electric sensor counting, but the cost is high; histopathology combined with in situ hybridization technology, but needs to analyze the tissue, which is not suitable for early screening work; hybrid capture DNA analysis, but only limited 13 kinds of HPV can be detected, and HPV typing cannot be carried out; HPV DNA detection in blood circulation, its disadvantage is that the positive rate of detection is not high, which is about 20%, and it is not high in women with current infection Less than half of them can detect the same subtype of HPV in serum and cervical specimens; in addition, Southern Blot is one of the methods for detecting HPV infection, but the operation is complicated and time-consuming, which is not conducive to large-scale screening

Among them, hybrid capture detection has high sensitivity, but its specificity is slightly lower than cytological screening, about 64.1%-95.1%. A comparative study of multiple screening methods for cervical cancer conducted by the Institute of Cancer, Chinese Academy of Medical Sciences, obtained The sensitivity and specificity of HPV-DNA capture method detection are 95% and 85%, respectively, but it cannot specifically distinguish high-risk and low-risk HPV subtypes; PCR detection has the highest sensitivity, and other methods can be used for specific classification type, broad-spectrum typing, but there is a problem of low specificity

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