A kind of treatment method of biological laboratory waste water

A treatment method and laboratory technology, applied in the field of biological laboratory wastewater treatment, can solve the problems of weak and unreliable degradation and removal of organic pollutants, and insufficient treatment efficiency of cancer cells, so as to avoid the generation of toxic disinfection by-products. , the effect of low health risk, low environmental toxicological ecological risk

Inactive Publication Date: 2012-02-01
FUDAN UNIV
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AI Technical Summary

Problems solved by technology

Practice has shown that the use of conventional disinfection methods (such as liquid chlorine disinfection) can inactivate bacteria in wastewater, but the treatment efficiency for viruses, gene fragments, and cancer cells is not high enough, and chlorine disinfection methods will produce chlorination disinfection side effects. Products and other environmental and ecological harmful substances
UV disinfection will not form toxic disinfection by-products, but the treatment efficiency of bacteria, viruses, gene fragments and cancer cells in wastewater is not high enough, and the germs after UV disinfection are easy to revive after exposure to light, which is not very reliable
In addition, methods other than ozone have weak degradation and removal functions for organic pollutants.

Method used

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  • A kind of treatment method of biological laboratory waste water

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Effect test

Embodiment 1

[0025] Wastewater from a biological laboratory in a university, the biological laboratory wastewater first enters the storage tank for flow regulation, and then enters the pH adjustment tank, where dilute H is added 2 SO 4 (30%) is used as a pH regulator to reduce the pH of the waste water to 2, stir for 20 minutes, and carry out preliminary sterilization; after sterilization, the waste water enters the chemical reaction tank, and CaO with a molar concentration of 0.75 times the LAS is added, and the medium speed (150r / min) Mix at high speed for 5 minutes for chemical reaction; the mixed water flows into the mixing tank for coagulation, add PAFCS 40mg / L, stir quickly (300r / min) for 1min, enter the flocculation tank after coagulation, add PAM 1.0mg / L, slowly (60r / min) stirred for 15min, entered the first sedimentation tank, then precipitated for 100min, and after solid-liquid separation, the supernatant continued to enter the Fenton oxidation tank, adding 0.044mol / L of H 2 o ...

Embodiment 2

[0029] A biological product development laboratory, water quality parameters COD 1550mg / L, LAS 25mg / L, pH7.5, turbidity 200NTU, SS60mg / L, NH 3 -N 4.3mg / L, T-N 7.1mg / L, T-P 0.36mg / L, the total number of bacteria 2.4×10 5 CFU / mL, ATP9.8×10 3 RLU / mL, biological toxicity is toxic. The water sample of biological laboratory wastewater first enters the storage tank for flow adjustment, and then enters the pH adjustment tank, and dilute H is added to the pH adjustment tank. 2 SO 4 (30%) as a pH regulator to lower the pH of the waste water to 2, stir for 10 minutes, and carry out preliminary sterilization, then enter the chemical reaction tank, add CaO with an amount of 1.0 times the molar concentration of LAS, quickly mix for 10 minutes, and pump the mixed water into Add PAFCS50mg / L to the mixing tank, stir rapidly (300r / min), stir for 1min, enter the flocculation tank after coagulation, add PAM0.5mg / L, stir slowly (60r / min), stir for 15min, and then let it settle 30min. After so...

Embodiment 3

[0032] The laboratory wastewater of the Medicine Valley Building in a high-tech park. The water samples of the laboratory wastewater first enter the storage tank for flow adjustment, and then enter the pH adjustment tank, and add dilute H to the pH adjustment tank. 2 SO 4 (30%) as a pH regulator to reduce the pH of the waste water to 2, stir for 30 minutes, and carry out preliminary sterilization, then enter the chemical reaction tank, add CaO with an amount of 1.0 times the molar concentration of LAS, quickly mix for 10 minutes, and pump the mixed water into In the mixing tank, add PAFCS60mg / L, stir rapidly (300r / min), stir for 1min, stir slowly (60r / min), stir for 15min, enter the flocculation tank after coagulation, add PAM0.8mg / L, and then let it settle 30min. After solid-liquid separation, the supernatant continues to enter the Fenton oxidation pool, adjust the pH of the water body in the oxidation pool to 3.5, react for 4 hours, mol (H 2 o 2 ) / mol(Fe 2+ ) ratio of 20...

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Abstract

The invention belongs to the technical field of environmental protection and relates to a biological laboratory wastewater treatment method. The present invention adopts the addition of pH regulator dilute H2SO4 30% as the primary sterilization method, and the chemical reaction agent calcium oxide, polyaluminium iron bis-acid coagulant and coagulant polyacrylamide are used together as coagulation precipitation to remove pollution in biological laboratory wastewater The material method, combined with the Fenton oxidation method, achieves the purpose of continuously reducing organic pollutants CODcr and LAS, and at the same time has the effect of killing bacteria, removing ATP, and reducing biological toxicity. The pH of the effluent is adjusted by calcium hydroxide, and after precipitation, the Measured, CODCr, LAS concentration, NH3-N, T-N, T-P in the biological laboratory wastewater discharged reached or exceeded the secondary discharge standard; the removal rate of the total number of bacteria reached 100%, the bacterial biological activity index value was 0, and the biological toxicity index The inhibition rate of luminescent bacteria is less than 30%.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a treatment method for biological laboratory wastewater. Background technique [0002] Bio-economy has become another new economic growth point in our country. Bio-science and technology have developed rapidly in the pharmaceutical industry, medical industry, agriculture, etc., and are widely used. They play an indispensable role in alleviating the contradictions of population, resources and environment faced by mankind missing roles. But its own potential threats and risks have increasingly aroused people's general attention and vigilance. According to the survey, the biologically active substances produced in the biological laboratory, the genetically engineered bacteria, viruses and cancer cells obtained by mutating or constructing organisms through artificial mutagenesis and genetic engineering in the laboratory, as well as the biological substan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/04C02F1/66C02F1/52C02F1/72
Inventor 刘燕杨楠桢李怀正金伟叶建锋刘翔代瑞华史舟吴金键张云蔡璇郭思
Owner FUDAN UNIV
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