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Smooth-surfaced gelatin microsphere and preparation method

A technology with smooth surface and gelatin, which is applied in the field of smooth surface gelatin microspheres and preparation, can solve the problems of poor surface smoothness of gelatin microspheres, large consumption of surfactants, organic solvents and oils, and achieves efficient preparation and simple preparation process Effect

Inactive Publication Date: 2010-11-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the surface smoothness of gelatin microspheres prepared by the method of acetone dehydration is not good, and surfactants or a large amount of organic solvent and oil phase are required in the preparation process.

Method used

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  • Smooth-surfaced gelatin microsphere and preparation method
  • Smooth-surfaced gelatin microsphere and preparation method
  • Smooth-surfaced gelatin microsphere and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dissolve 10g of gelatin in 25ml of 70°C distilled water to prepare a solution with a concentration of 0.4g / ml; add 150ml of liquid paraffin into a 500ml round bottom flask, keep it in a 70°C water bath for 60 minutes, and make the temperature reach 70°C; stir it mechanically (3000r / min), pour the gelatin aqueous solution into the above-mentioned 70°C liquid paraffin, and emulsify for 15 minutes; immediately pour the above-mentioned mixed solution into 100ml of pre-cooled (-10~0°C ice-salt bath), stir and cool After reaching below 0°C (60min), add 100ml 75% ethanol (-18°C) to it, stir and dehydrate for 60min, stop stirring and let it stand for 10min to obtain gelatin microspheres at the bottom of the beaker; after 85% ethanol, 95% ethanol, 100% Each 100ml of ethanol was washed three times (15min / time) to remove residual liquid paraffin; air-dried for 3 days to obtain gelatin microspheres. The SEM photo (a) and particle size distribution figure (b) of the resulting gelati...

Embodiment 2

[0018] Dissolve 20g of gelatin in 50ml of 70°C distilled water to prepare a solution with a concentration of 0.4g / ml; add 300ml of liquid paraffin into a 500ml round bottom flask, keep it in a 70°C water bath for 60 minutes, and make the temperature reach 70°C; stir it mechanically (3000r / min), pour the gelatin aqueous solution into the above-mentioned 70°C liquid paraffin, and emulsify for 15 minutes; pour the above-mentioned mixture into a beaker that has been placed in an ice-salt bath (-10~0°C), stir and cool to 0 Below ℃ (60min), add 200ml of 75% ethanol (-18℃) to it, stir and dehydrate for 60min, and get gelatin microspheres at the bottom of the beaker 10min after the stirring stops; wash with 200ml each of 85% ethanol, 95% ethanol, and 100% ethanol Three times (15min / time) to remove residual liquid paraffin; air-dried for 3 days to obtain gelatin microspheres. The SEM photo (a) and particle size distribution figure (b) of the resulting gelatin microspheres are as follow...

Embodiment 3

[0020] Dissolve 3g of gelatin in 20ml of 37°C distilled water to prepare a solution with a concentration of 0.15g / ml; add 200ml of liquid paraffin into a 500ml round bottom flask, keep it in a 37°C water bath for 60 minutes, and make the temperature reach 37°C; stir it mechanically (3000r / min), add gelatin aqueous solution dropwise into liquid paraffin at 37°C, and emulsify for 15 minutes; pour 100ml of liquid paraffin pre-cooled (in an ice-salt bath at -10 to 0°C) into the above mixed solution immediately, and solidify the microspheres After the above mixed solution is cooled to below 0°C (60min), add 100ml of 85% ethanol (-18°C), stir for 60min to dehydrate, and get gelatin microspheres at the bottom of the beaker 10min after the stirring stops; after 90% ethanol, 95% ethanol 100ml each of 100% ethanol was washed three times (15min / time) to remove residual liquid paraffin; air-dried for 3 days to obtain gelatin microspheres. The SEM photo (a) and particle size distribution f...

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Abstract

The invention relates to a smooth-surfaced gelatin microsphere and a preparation method. The preparation method comprises the following preparation steps of: under stirring, uniformly mixing a gelatin aqueous solution with liquid olefin at the temperature of 37-70 DEG C, emulsifying for 5-50 minutes, then mixing the mixed liquid with the liquid olefin at the temperature of minus10-0 DEG C, mechanically stirring, adding 75% (minus18-0 DEG C) of ethanol, stirring, settling for more than 10 minutes, and washing the gelatin microsphere three times through the ethanol (50-100% of ethanol) with gradient concentration to further dehydrate and wash out the liquid olefin remained on the surface of the microsphere; and finally carrying out air drying for a period of time to obtain the gelatin microsphere. The invention has the advantages that the gradient ethanol is used for dehydration after emulsification, and the smooth-surfaced gelatin microsphere is obtained under the condition of not using a surfactant. The invention has simple preparation process, no need of an organic solvent and fewer consumed oil phases, the smooth-surfaced gelatin microsphere can be obtained as long as the gradient ethanol is used for dehydration and other preparation parameters are changed, and a simple and easy method is supplied for efficiently preparing the smooth-surfaced gelatin microsphere.

Description

technical field [0001] The invention relates to a gelatin microsphere with a smooth surface and a preparation method thereof. Background technique [0002] The oil-in-water (W / O) single emulsion method can be used to prepare gelatin microspheres, wherein the water phase is an aqueous solution of gelatin, and the oil phase is usually mineral oil, olive oil, etc., and the formed W / O emulsion is cooled, and then used Acetone or the like replaces the water in the gelatin microspheres while washing away the residual oil on the surface of the microspheres. Gelatin is a hydrolyzed product of collagen, its chemical composition is very similar to collagen, and it has good biocompatibility. The prepared gelatin microspheres can be used as drug carriers and porogens for tissue engineering porous scaffolds. Gelatin microspheres can also modify the surface of the scaffold in situ, and have broad application prospects in the field of tissue engineering. [0003] Usually, when gelatin mi...

Claims

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Application Information

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IPC IPC(8): C08J3/00C08J3/12C08L89/00
Inventor 袁晓燕唐功文
Owner TIANJIN UNIV
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