Bacillus subtilis with bio-control effect on continuous cropping diseases of strawberries and application thereof

A technology of Bacillus subtilis and biological inoculants, applied in the directions of application, microorganism-based methods, and chemicals for biological control, etc., can solve the problems of continuous cropping diseases of strawberries and the inability of standardized crop rotation in production areas, and achieves less bacterial side effects. , pathogenic bacteria are really strong, and the effect of enhancing air permeability

Inactive Publication Date: 2010-11-24
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main strawberry planting areas in the world generally adopt the one-year planting system, but in my country, due to the limited number of arable land and the

Method used

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  • Bacillus subtilis with bio-control effect on continuous cropping diseases of strawberries and application thereof
  • Bacillus subtilis with bio-control effect on continuous cropping diseases of strawberries and application thereof
  • Bacillus subtilis with bio-control effect on continuous cropping diseases of strawberries and application thereof

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1, the isolation and identification of bacteria

[0028] 1. Isolation of bacteria

[0029] Using the five-point method, the strawberry rhizosphere soil was taken from the strawberry production field, put in a sterilized aluminum box, and brought back to the laboratory. Weigh 20g of soil sample and add 90ml of sterile water, absorb the supernatant to make a dilution of 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 soil solution. Pick 10 -3 , 10 -4 , 10 -5 For each of the three dilutions, draw 0.1 ml of the solution to coat the plate, and place it upside down in an artificial incubator for cultivation at 370°C. Bacteria with different phenotypes were streaked on the plate medium with an inoculation needle, and then cultured at 37°C. Bacteria isolation medium is: beef extract 5g / L, peptone 10g / L, NaCl 5g / L, agar powder 10g / L, pH 7.2. The agar blocks of pathogenic fungi (Fusarium oxysporum or Rhizoctonia solani) were inserted into one side of the plate by the ...

Embodiment 2

[0038] Embodiment 2, the application of bacteria

[0039] 1. Antagonistic effect of strain ZLS-01 on pathogenic fungi

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Abstract

The invention discloses bacillus subtilis with a bio-control effect on continuous cropping diseases of strawberries and application thereof. The bacillus subtilis is bacillus subtilis ZLS-01, and the preservation number of the bacillus subtilis is CGMCC No. 3513. Experiments prove that the bacillus subtilis has an antagonistic effect on fusarium oxysporum or rhizoctonia solani and can inhibit diseases. The bacillus subtilis inoculum has the advantages of increasing the number of predominant bacteria in soil, playing a positive role in preventing and inhibiting soil-borne diseases, reducing the pH value of the soil from 8.2 to 7.1, making the PH value tend to be neutral, enhancing the acid-base buffer capacity, the air permeability, the water permeability, the water-retaining property and the nutrition-retaining property of the soil, increasing organic matters of the soil from 2.80 percent to 3.20 percent year by year so as to improve soil fertility, obviously increasing alkaline hydrolytic nitrogen, rapid available phosphorus and rapid available potassium of rhizospheric soil of the strawberries, inhibiting continuous cropping diseases of the strawberries and guaranteeing the output of the strawberries.

Description

technical field [0001] The invention relates to a bacillus subtilis which plays a role of biological control in strawberry continuous cropping diseases and its application. Background technique [0002] Continuous cropping diseases lead to poor growth and development of many crops, including strawberries, serious root diseases, yield and quality decline, and yield reductions as high as 50% to 90%. In the past, people used various methods such as cultivation, physics, chemistry and microbial preparations to overcome continuous cropping diseases, among which chemical drugs such as methyl bromide were the most common methods to treat soil. However, the toxicity of chemical drugs poses a serious threat to human health, and it is inevitable to ban their use. Cultivation and physical methods such as: crop rotation, soil drying, soil fumigation or steaming disinfection can achieve certain effects, but there are practical difficulties and disadvantages. For example, due to the actu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C09K17/00C12R1/125C09K101/00C09K109/00
Inventor 张潞生
Owner CHINA AGRI UNIV
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