Biomolecule interaction detection method and system based on SPR interference imaging
A technology of biomolecules and interference imaging, applied in the biological field, can solve problems such as difficult to achieve high-precision detection, deformation of electro-optic crystal structures, measurement errors, etc.
Inactive Publication Date: 2010-12-15
TSINGHUA UNIV
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However, when a high voltage is applied to the electro-optic crystal, the inverse piezoelectric effect will appear, causing micro-scale deformation of the structure of the electro-optic crystal, causing a small shift in the optical path, that is, the position of the spot moves, directly generating measurement errors and affecting the array. The consistency of detection is poor, and it is difficult to meet the requirements of high-precision detection
This is an inherent defect of electro-optic crystals that cannot be remedied
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[0034] The method for detecting biomolecular interactions based on SPR interferometric imaging and the system for detecting biomolecular interactions based on SPR interferometric imaging described in the present invention will be illustrated below with reference to the accompanying drawings.
[0035] The calculation method of the present invention utilizes the characteristics that the amplitude and phase of the SPR reflected light vary dramatically with the refractive index, and the signal diagram A reflecting the refractive index distribution calculated by formula (1) and the actual refractive index relationship can be obtained through Fresnel Er formula simulation, the simulation results are as follows image 3 shown. It can be seen from the figure that there is a linear relationship between the two in a certain interval. Therefore, the change of A can reflect the actual change of the refractive index, and the position of the linear interval is determined by the size of the...
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The invention discloses a biomolecule interaction detection method comprising the following steps: 1) obtaining collimated light; 2) alternately modulating the phase difference between the P polarized component and the S polarized component of the collimated light to 90 degrees or -90 degrees; 3) allowing phase modulation light to enter into a biological sensing unit so as to stimulate surface plasma resonance, and reflecting light; 4) interfering the P polarized component with the S polarized component in the reflected light to generate an interference image; 5) imaging the interference image, and collecting the imaging interference image; 6) alternately collecting the imaging interference image generated after adding phase differences of 90 degrees and -90 degrees; and 7) utilizing the collected adjacent two frames of imaging interference images to calculate a distribution function reflecting the distribution of refractive index. The invention can continuously measure to obtain the refractive index distribution change information along with time in real time.
Description
technical field [0001] The present invention relates to the field of biotechnology, in particular to high-throughput, high-precision, label-free, real-time sensing protein-protein, protein-DNA, protein-effector, antigen-antibody, ligand-receptor, drug- A detection method and a detection system for the interaction of biomolecules such as targets. Background technique [0002] The essence of life process is a process of interaction of various biomolecules, and the occurrence and change of diseases are all related to molecular interactions. Because of this, the detection of biomolecular interactions has become one of the research hotspots in life sciences. Among them, protein is the product of gene expression, which performs the functions of life. The study of protein molecules and their interactions with other molecules is of great significance for elucidating the mechanism of life processes and disease occurrence and development, as well as for the discovery of new drugs. ....
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IPC IPC(8): G01N21/45
CPCG01N21/553G01N21/45
Inventor 王大千邓焱罗昭锋余兴龙
Owner TSINGHUA UNIV