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Method for controlling plant type of rice

A rice plant type and purpose technology, applied in the field of plant genetic engineering, can solve the problem of rice tiller number decline and other issues

Inactive Publication Date: 2010-12-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overexpression of OsTB1 leads to a sharp decrease in the number of rice tillers, basically no tillers, or only 1-2 tillers, but the axillary buds on the stem nodes can be seen when the leaves are peeled off, indicating that the formation of axillary buds has not been affected, but cannot be elongated to form normal tiller

Method used

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  • Method for controlling plant type of rice
  • Method for controlling plant type of rice
  • Method for controlling plant type of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Construction of the rice gene OsTB1 promoter-driven vector pTB1:156e of the osa-miR156e gene

[0032] The constitutive expression of miR156 has an important impact on rice plant architecture: the number of tillers increases greatly, the plants become shorter, the heading becomes later, the panicles become smaller, etc., the seed setting rate decreases, and the yield decreases. The invention drives the expression of miR156 in lateral meristems and other parts through a specific expression promoter, hoping to improve rice tillering ability without affecting other traits, achieve the purpose of improving plant type, and then apply it to rice breeding practice.

[0033] The promoter of the specific expression gene OsTB1 used is mainly expressed in the axillary buds, and also expressed at the base of the top growth point of the stem, the vascular tissue of the stem pith and the vascular tissue of the leaf junction.

[0034] The rice osa-miR156 gene family has 12 m...

Embodiment 2

[0069] Example 2. Genetic transformation and expression of the osa-miR156e gene under the regulation of the OsTB1 promoter

[0070] The pTB1:156e vector was introduced into the rice variety "Zhonghua 11" using the genetic transformation method mediated by Agrobacterium EHA105 (Wu et al., Development of enhancer trap lines forfunctional analysis of the rice genome. Plant J, 2003, 35: 418-427) superior.

[0071] 1) Callus induction

[0072] (1) Ripe rice seeds are dehulled, then treated with 70% ethanol for 1 minute, and 0.15% mercuric chloride for 15 minutes;

[0073] (2) Wash the seeds with sterilized water 4-5 times;

[0074] (3) seeds are placed on the induction medium;

[0075] (4) Culture in a dark place for 5 weeks at a temperature of 25-27°C.

[0076] 2) Callus subculture

[0077] Select bright yellow, compact and relatively dry embryogenic calli, and place them on the subculture medium for 2 weeks in the dark at a temperature of 25-27°C.

[0078] 3) Pre-culture

[...

Embodiment 3

[0104] Example 3. Improvement and application of rice plants transfected with pTB1:156e to plant type

[0105] Among all the transgenic plants transgenic for pTB1:156e, one T0 generation with the best phenotype was selected and planted as a single plant for the next generation. The agronomic traits such as tiller number and yield of a single plant were investigated under different planting densities ( Figure 4 ).

[0106] Planting Design:

[0107] Planting material: select a single-copy line, control species ZH11 and constitutively overexpressed osa-miR156e plant planting density 3 kinds:

[0108] (1) Thin planting: 8 inches x 8 inches (26.7cm x 26.7cm)

[0109] (2) Normal density: 5 inches x 8 inches (16.7cm x 26.7cm)

[0110] (3) Close planting: 5 inches x 5 inches (16.7cm x 16.7cm)

[0111] Each plot: plant 3-5 rows, 10 plants in each row. 2 trials were repeated. Seed test: Take the 3rd to 7th plants in the middle row of the plot, and take 5 plants in a row.

[011...

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Abstract

The invention relates to the field of plant genetic engineering, in particular to a method for controlling the plant type of rice by using molecules. The method is characterized in that the plant type of the rice is controlled by controlling the expression mode of an osa-miR156e gene or other homologous genes osa-miR156a and osa-miR156I in the gene family of the osa-miR156e gene by using the promoter of a specific expression gene OsTB1. The DNA sequence of the promoter of the invention is a sequence SEQ ID No.1. The DNA sequence of the osa-miR156e gene is a sequence SEQ ID No.2. In the invention, the plant type of rice is controlled by a molecular design process; and the method is expected to be used in rice plant type breeding to built ideal plant type and improve rice yield.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. In particular, it relates to a method for regulating rice plant type by a molecular design method. The method of the present invention is to regulate the expression of the rice osa-miR156e gene or its homologous gene through the promoter of the specific expression gene OsTB1, so as to achieve the purpose of regulating the plant type of rice, and using the method in the application of transgenic rice and transgenic rice seeds, Shape the ideal plant type of rice and increase the yield of rice. Background technique [0002] As an important food crop, rice is the staple food of more than one-third of the world's population. At the same time, rice is regarded as a model plant for genome research and genetic improvement due to its small genome, fine genetic and physical maps, relatively easy transgenic technology, and synteny with other grass crops. In order to solve the contradiction betwee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/113A01H5/00
Inventor 吴昌银陈志辉张启发
Owner HUAZHONG AGRI UNIV
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