Preparation method and applications of ant polypeptide

A technology of ants and ants dry powder, which is applied in the biological field, can solve the problems of unfavorable ant polypeptide industrial production, enzyme selection, and enzymatic hydrolysis conditions without substantive research, and achieve good solubility, low cost, and quick results. Effect

Inactive Publication Date: 2010-12-29
WUHAN TAIJI SANFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent "ant ginseng oral liquid and its preparation method" (CN1136931) introduces a method of enzymatic hydrolysis of ant protein, which uses protease to decompose the protein in the ant residue after water extraction and alcohol extraction into amino acids and polypeptid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 single enzyme hydrolysis method

[0038] Wash 500 grams of ants, dry them at 40°C for 8 hours, and with a water content of less than 10%, crush and sieve them to make 40-mesh dry ant powder, then place the dry ant powder in food-grade CO with a concentration of 99.5% by mass. 2 In the supercritical extractor, adjust the extraction solvent CO 2 The flow rate is 20L / h, the extraction pressure is 30Pa, and the temperature is 45°C, the extraction is carried out for 1 hour, and the ant oil and ant protein are separated and collected; the defatted ant protein is poured into the enzymatic hydrolysis tank. When the amount of enzyme added is 4000u of neutral protease to enzymatically hydrolyze ant protein, the enzymatic hydrolysis conditions are temperature 50°C, the pH value is adjusted to 6 with a calcium hydroxide solution with a mass percentage concentration of 10%, the enzymatic hydrolysis time is 3 hours, and the substrate concentration 3%; after the enzymati...

Embodiment 2

[0039] Embodiment 2 single enzyme hydrolysis method

[0040] Wash 500 grams of ants, dry them at 60°C for 6 hours, and have a water content of less than 10%, crush them and sieve them to make 80-mesh dry ant powder, then place the dry ant powder in food-grade CO with a concentration of 99.9%. 2 In the supercritical extractor, adjust the extraction solvent CO 2 The flow rate is 10L / h, the extraction pressure is 35Pa, and the temperature is 50°C, the extraction is carried out for 2 hours, and the ant oil and ant protein are separated and collected; the defatted ant protein is poured into the enzymatic hydrolysis tank. When the amount of enzyme added is 5000u papain to enzymolyze ant protein, the enzymolysis conditions are temperature 55°C, the pH value is adjusted to 7 with a calcium hydroxide solution with a mass percentage concentration of 30%, the enzymolysis time is 7h, and the substrate concentration is 7.5 %; After complete enzymatic hydrolysis, inactivate the enzyme at 1...

Embodiment 3

[0041] Embodiment 3 single enzyme hydrolysis method

[0042] Wash 1,000 grams of ants, dry them at 50°C for 6 hours, and keep the water content less than 10%, crush them and sieve them to make 60-mesh dry ant powder, then place the dry ant powder in food-grade CO with a concentration of 99.8%. 2 In the supercritical extractor, adjust the extraction solvent CO 2 The flow rate is 15L / h, the extraction pressure is 10Pa, and the temperature is 40°C, the extraction is carried out for 3 hours, and the ant oil and ant protein are separated and collected; the defatted ant protein is poured into the enzymatic hydrolysis tank. When the amount of enzyme added is 4000u bromelain to enzymatically hydrolyze ant protein, the enzymatic hydrolysis conditions are temperature 40°C, the pH value is adjusted to 6.5 with a calcium hydroxide solution with a mass percentage concentration of 20%, the enzymatic hydrolysis time is 3.5h, and the substrate concentration is 7 %; After the enzymatic hydrol...

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PUM

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Abstract

The invention relates to a preparation method and applications of ant polypeptide. The invention mainly overcomes the defects of difficult digestion and absorption and poor processing performance of ant protein. The method comprises the following steps: firstly, cleaning ants, drying the ants at low temperature, and crushing and sieving the ants to prepare dry ant powder; carrying out degreasing treatment by utilizing a supercritical CO2 extraction technology to prepare the ant protein; carrying out enzymolysis on the degreased ant protein by protease; then inactivating enzymes, sterilizing and filtering; carrying out vacuum concentration; and freeze-drying or spray-drying to obtain ant polypeptide powder. The ant polypeptide prepared according to the invention has stable properties and good processing performance, can be stored easily, and can be widely used for medicines, health care products, food raw materials or food additives; and because the ant polypeptide has low cost, less investment, high effect taking speed and high additional value, the ant polypeptide is suitable for industrialized production.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a preparation method and application of an ant polypeptide. Background technique [0002] Ants belong to Arthropoda, Insecta, Hymenoptera, Formicaceae, and there are many kinds of them. There are more than 16,000 species in the world, and it is estimated that there are more than 2,000 species in my country. At present, there are less than 20 kinds of ants in clinical application, among which 10 kinds of ants that have been named and commonly used as medicine are: Rhizoma ant, Red-tailed ant, Japanese archer ant, Yellow scavenger ant, Black mountain ant, Red forest ant, and double-toothed ant Prickly ants, pseudo-black thorny ants (also known as Dingtu thorny ants), giant leaf-cutter ants and double-bone pavement ants. Formally approved by the State Food and Drug Administration as new resource food and new resource traditional Chinese medicine, the ant species that can be use...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A23L1/29A23L1/30A61P3/10A61P15/08A61P17/14A61P19/10A61P25/28A61P37/04A61P39/06A23L33/18
CPCY02P20/54
Inventor 涂传荣刘学铭胡建平陈海锋刘军华刘安和
Owner WUHAN TAIJI SANFENG BIOTECH
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