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Method and special chemical luminous immunoassay kit for detecting clenbuterol

A chemiluminescence immunization and kit technology, applied in microorganism-based methods, biological tests, immunoglobulins, etc., can solve problems such as harm to public health, severe side effects of clenbuterol, residues in livestock and poultry products, etc., and achieve preservation. The effect of good stability and stability, high sensitivity and high specificity

Active Publication Date: 2011-01-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, clenbuterol has relatively high toxicity and side effects, and countries such as the European Union and China have banned its use as a feed additive. Some criminals still use it in animal husbandry production regardless of the health and safety of consumers, resulting in its use in livestock and poultry products. Residues, endangering public health

Method used

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  • Method and special chemical luminous immunoassay kit for detecting clenbuterol
  • Method and special chemical luminous immunoassay kit for detecting clenbuterol
  • Method and special chemical luminous immunoassay kit for detecting clenbuterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, preparation and detection method of chemiluminescent immunoassay kit

[0040] 1. The chemiluminescent immunoassay kit includes:

[0041] (1) Original coating solution: obtained by dissolving the original coating in the coating buffer, wherein the concentration of the original coating in the original coating solution is 0.08 μg / mL; the original coating is the clenbuterol hapten Conjugates with ovalbumin.

[0042] (2) Horseradish peroxidase-labeled goat anti-mouse anti-antibody working solution:

[0043] Dilute horseradish peroxidase-labeled goat anti-mouse anti-antibody with diluent, the dilution ratio is 1:2000;

[0044] The diluent is obtained by mixing 50mL bovine serum albumin and 950mL phosphate buffer; the concentration of the phosphate buffer is 0.02M, and the pH value is 7.4.

[0045] Horseradish peroxidase-labeled goat anti-mouse anti-antibody was purchased from Jackson ImmunoResearch Laboratories Inc. catalog number 115-035-003

[0046](3) Cle...

Embodiment 2

[0099] Embodiment 2, test kit sensitivity, accuracy and shelf life test

[0100] 1. Kit sensitivity experiment

[0101] The zero standard solution (that is, the diluent is pH7.4, 0.05M phosphate buffer) was tested 20 times, and the average value of the measurement results plus 3 times the standard deviation was used as the minimum detection limit of the kit.

[0102] Table 1 Statistical table of zero standard measurement results μg / L

[0103]

[0104] It can be seen from Table 1 that the minimum detection limit of the kit is 0.1 μg / L.

[0105] 2. Standard product precision test:

[0106] From the three batches of kits described in Example 1 (batch 01, batch 02, and batch 03), 10 kits were extracted from each batch, and the luminous intensity value of the 1 μg / L standard solution was measured to calculate the coefficient of variation. The detection method is consistent with that described in Experiment 3 in Example 1.

[0107] The experiment was repeated three times, and...

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Abstract

The invention discloses a method and a special chemical luminous immunoassay kit for detecting clenbuterol. The special chemical luminous immunoassay kit for detecting clenbuterol comprises a clenbuterol specific antibody, a coating antigen and standard solution; and the coating antigen is a coupling compound of a clenbuterol hapten and a carrier protein. The detection method has the characteristics of simple sample preprocessing process, simple and convenient operation, low cost, high specificity, high sensitivity, high accuracy and the like, can perform monitoring on site and is suitable for screening a large number of samples.

Description

technical field [0001] The invention relates to a method for detecting clenbuterol and a special chemiluminescent immunoassay kit. Background technique [0002] At present, the safety of animal food has become the focus of attention all over the world, and the problem of veterinary drug residues is one of the most important factors affecting the safety of animal food. Due to the complex composition of animal samples, the concentration of analytes is low, and most of the samples are very small , which puts forward higher requirements on the selectivity and sensitivity of the analytical method. Chemiluminescence immunoassay (Chemiluminescence analysis, CLIA) technology combines highly sensitive chemiluminescence technology with highly specific immune reaction, which has high sensitivity, strong specificity, wide linear range, easy operation, and does not require very expensive equipment Features. CLIA does not require external light sources, has a higher signal-to-noise rati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/577G01N33/531C07K16/44C12N5/20C12R1/91
Inventor 程林丽沈建忠张素霞王战辉史为民吴聪明曹兴元汤树生
Owner CHINA AGRI UNIV
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