Method for rapidly detecting content of aflatoxin

A kind of aflatoxin content technology, which is applied in the direction of material analysis by observing the influence on chemical indicators, fluorescence/phosphorescence, analysis by making materials undergo chemical reactions, etc., can solve the problems of high detection cost and complicated detection steps , to achieve the effects of overcoming hazards and pollution, lowering detection limits, and less environmental pollution

Inactive Publication Date: 2012-10-17
云南健牛环境监测有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this type of kit still has problems such as high detection cost and complicated detection steps.

Method used

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  • Method for rapidly detecting content of aflatoxin
  • Method for rapidly detecting content of aflatoxin
  • Method for rapidly detecting content of aflatoxin

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Detect whether aflatoxin B1 in the early compound feed of piglets exceeds the standard. According to GB13078-2001 "Feed Hygiene Standard", the compound feed and concentrated feed for meat piglets are ≤10μg / kg. Calculation formula of sample Mg: Mg=(0.04μg / ml×1ml) / 10μg / kg, Mg=4g. Take 4g of the sample to be tested that has been ground through a 20-mesh sieve and put it in a 100ml beaker, add 10ml of chloroform solution, shake for 2min, and filter with qualitative filter paper. The filtrate was heated and concentrated in a water bath at 70°C, volatilized to about 0.5ml, and made up to 1ml with chloroform (referred to as the test product). On a thin-layer silica gel plate with a length of 10 cm, 30 μl each of the test sample and aflatoxin B1 standard substance (5 μl each time, 6 times in total), and then add oxalic acid with a concentration of 1.5% by weight and volume. 10 μl as derivatization reagent. After being evaporated to dryness, it was put into a chromatographic ...

Embodiment 2

[0030] Detection of aflatoxin B1 in the mid-term compound feed of 212 growing chickens. According to GB13078-2001 "Feed Hygiene Standard", it is stipulated that the compound feed and concentrated feed of broiler chickens, growing chickens, laying hens should be less than 20μg / kg. Calculation formula of sample Mg: Mg=(0.20μg / ml×1ml) / 20μg / kg, Mg=10g. Take 10g of the sample to be tested that has been ground through a 20-mesh sieve and put it in a 100ml beaker, add 20ml of dichloromethane solution, shake for 5min, and filter with quantitative filter paper. The filtrate was heated and concentrated in a water bath at 65°C, evaporated to about 1ml, and the volume was adjusted to 2ml with dichloromethane (referred to as the test product). On a thin-layer silica gel plate with a length and width of 12cm, 40μl each of the test sample and aflatoxin standard B1 (5μl each time, a total of 8 times), and then add 3% derivatization reagent dilute hydrochloric acid. 15 μl. After being evapo...

Embodiment 3

[0032] Detect whether aflatoxin B1 in corn exceeds the standard. According to GB2715-2005 "Food Hygiene Standard", the maximum allowable content of aflatoxin B1 in corn is ≤20μg / kg. Calculation formula of sample Mg: Mg=(0.20μg / ml×1ml) / 20μg / kg, Mg=10g. Take 10g of the sample to be tested that has been ground through a 20-mesh sieve and put it in a 100ml beaker, add 30ml of isopropanol solution to ultrasonic for 5min, and filter with qualitative filter paper. The filtrate was heated and concentrated in a water bath at 80°C, volatilized to about 2ml, and made up to 3ml with isopropanol (referred to as the test product). On a thin-layer silica gel plate with a length of 15cm, 24μl each of test sample ① and standard B1 (6μl each time, 4 times in total), and then add 5μl of 2% derivatizing reagent sulfuric acid. After being evaporated to dryness, put it into a hexane chromatographic cylinder filled with solvent to carry out a development, the front of the solvent is developed 10cm...

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Abstract

The invention discloses a method for rapidly detecting the content of aflatoxin. The method comprises the following steps of: after smashing a sample to be detected, putting the sample powder into a beaker, and adding an extractant for shaking or performing ultrasonic extraction; filtering, performing water bath evaporation on a filtrate, and metering the volume to obtain a test sample; respectively pointwise adding the test sample and an aflatoxin B1 standard substance which are commensurate on a thin-layer silica gel plate, pointwise adding derivative reagents into sampling points, and respectively developing by developers; and comparing the fluorescence intensity of the test sample and the aflatoxin B1 standard substance under an ultraviolet lamp to judge whether the content of the aflatoxin in the test sample exceeds the standard. The method for rapidly detecting the content of the aflatoxin has the advantages of small solvent amount, simple sample treatment and short measurement time; the method does not need a professional, only needs small apparatus, and is particularly suitable for rapid field detection; and the method effectively avoids the harm and the pollution of trifluoroacetic acid to operators and the environment, improves the method sensitivity, and has the lowest detectable amount of 0.00032 mu g and the lowest detection limit of 3 mu g / kg.

Description

technical field [0001] The invention belongs to the technical field of food safety inspection, and in particular relates to a method for rapidly detecting the residual content of aflatoxin in food and feed. Background technique [0002] Aflatoxins are metabolites produced by Aspergillus flavus and Aspergillus parasiticus. When the grain is not dried in time and stored improperly, it is often easily contaminated by Aspergillus flavus or Aspergillus parasiticus to produce such toxins. The presence of aflatoxins in food and feed in hot and humid regions is high. Aflatoxin mainly contaminates feed, grain and oil food, animal and plant food, etc. Aflatoxin contamination occurs in feed, peanuts, corn, rice, wheat, beans, nuts, peppers, etc. Among them, feed, pepper, peanut and corn are the most seriously polluted. When people accidentally eat food contaminated with aflatoxins, it will cause harm to their health. At present, it is still difficult to prevent aflatoxin contaminat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/77G01N21/78G01N21/64
Inventor 杨亚玲吕金玲李冉
Owner 云南健牛环境监测有限公司
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