Pig small intestine epithelial cell line and construction method thereof

A technology of epithelial cells and establishment methods, applied in the field of porcine small intestinal epithelial cell lines and their establishment, can solve the problems of small number of cell growth, inconvenient popularization and application, high production cost, etc., and achieve good growth status, low nutritional requirements, The effect of strong vitality
CN101955907AInactive Publication Date: 2011-01-26NORTHWEST A & F UNIV
3 Cites 21 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2011-01-26
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a pig small intestine epithelial cell line (ZYM-SIEC02), which was preserved in China Center for Type Culture Collection on January 13th, 2010 with a collection number of CCTCC-C201001. The invention also discloses the construction method of the cell line. The cell line has the advantages of low nutrient condition requirements, quick cell growth, easy cultivation and convenient promotion and application.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of animal cell engineering and relates to the establishment of a new cell line, in particular to a pig small intestinal epithelial cell line (ZYM-SIEC02) and its establishment method. Background technique

[0002] Intestinal epithelial cells (IEC) are the main functional cells of the intestine, which participate in digestion, absorption, immune barrier and stress response of the intestine, and are closely related to the endocrine and exocrine functions of the intestine. Therefore, the isolation and culture of small intestinal epithelial cells is one of the main means to study small intestinal function, nutrient absorption mechanism and its regulation.

[0003] Instructor Zhang Yanming, College of Animal Science, Northwest Agriculture and Forestry University, and others successively worked on the isolation and culture method of pig umbilical vein endothelial cells, the preliminary study on the application of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More