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Method for extracting polysaccharide and polyphenol of oil-tea camellia husk

A technology of Camellia oleifera and Pu polyphenols, applied in the direction of pharmaceutical formulations, etc., can solve the problems of long time consumption, reduce the biological activity of polysaccharides, and the content of impurities in the extract, and achieve the effects of reducing production costs, reducing the amount of organic solvents, and retaining activity

Inactive Publication Date: 2012-01-04
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many methods for extracting polysaccharides and polyphenols from tea leaves, but there are few literature and patent reports about the processing of Camellia oleifera, and only Chinese patents CN101560265A and CN101560266A disclose the preparation method and purification method of polysaccharides from Camellia oleifera, but there are deficiencies : (1) the technological method gained only has a kind of active component of camellia oleifera polysaccharide, which causes a waste of resources; (2) the extraction of camellia oleifera polysaccharide adopts alcohol precipitation method, which needs to consume a large amount of organic solvent, takes a long time, and extracts impurities High content, complex post-treatment process; at the same time, it is easy to reduce the biological activity of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 100g of Camellia oleifera, after drying and crushing, pass through a 60-80 mesh sieve; weigh 10g of crushed Camellia oleifera, use 150mL of water as the extraction agent, extract at a stirring speed of 400rpm and a temperature of 80°C for 1.5h, and extract the liquid through The supernatant was obtained by centrifuging at 4000 rpm for 10 minutes; a total of 3 extractions were carried out according to the above conditions, and the crude extracts of polysaccharides and polyphenols of Camellia oleifera were obtained after combination.

[0025] Add ethanol and ammonium sulfate respectively, so that the mass concentration of ethanol in the formed two-phase extraction system is 25%, the mass concentration of ammonium sulfate is 20%, stir and extract for 30min, then stand for stratification. The ethanol phase, the lower layer is the ammonium sulfate aqueous phase containing Camellia oleifera polysaccharide.

[0026] The upper phase of ethanol obtained after phase separati...

Embodiment 2

[0037] Take 100g of Camellia oleifera, after drying and crushing, pass through a 80-100 mesh sieve; weigh 10g of crushed Camellia oleifera, use 100mL of water as the extraction agent, extract at a stirring speed of 300rpm and a temperature of 95°C for 1h, and extract the Centrifuge at 4000rpm for 10min to obtain the supernatant; extract twice according to the above conditions, and combine to obtain the crude extract of Camellia oleifera polysaccharides and polyphenols.

[0038] Add ethanol and dipotassium hydrogen phosphate respectively, so that the mass concentration of ethanol in the two-phase aqueous extraction system formed is 25%, and the mass concentration of dipotassium hydrogen phosphate is 18%, stir and extract for 30min, then stand and separate layers, and the upper layer contains The ethanol phase of Camellia oleifera polyphenols, the lower layer is the ammonium sulfate aqueous phase containing Camellia oleifera polysaccharides.

[0039] The upper phase of ethanol o...

Embodiment 3

[0042] Take 200g of Camellia oleifera, after drying and crushing, pass through a 100-120 mesh sieve; weigh 10g of crushed Camellia oleifera, use 100mL of water as the extraction agent, extract at a stirring speed of 300rpm and a temperature of 90°C for 1h, and extract the liquid by rotating speed Centrifuge at 4000rpm for 10min to obtain the supernatant; extract twice according to the above conditions, and combine to obtain the crude extract of Camellia oleifera polysaccharides and polyphenols.

[0043] Add ethanol and ammonium sulfate respectively, so that the mass concentration of ethanol in the formed two-phase extraction system is 25%, the mass concentration of ammonium sulfate is 20%, stir and extract for 30min, then stand for stratification. The ethanol phase, the lower layer is the ammonium sulfate aqueous phase containing Camellia oleifera polysaccharide.

[0044] The upper phase of ethanol obtained after phase separation was taken, concentrated in vacuo, washed with e...

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Abstract

The invention relates to a method for extracting polysaccharide and polyphenol of oil-tea camellia husk, which takes the oil-tea camellia husk as a raw material. The method comprises the following steps of: (1) putting dried and smashed oil-tea camellia husk into an extracting device, extracting under stirring by taking water as a leaching solvent and performing centrifugal separation so as to obtain crude extract of the polysaccharide and polyphenol of the oil-tea camellia husk; (2) adding ethanol and inorganic salt into the crude extract so as to form a double-water phase system, adding theoil-tea camellia husk polyphenol into an ethanol upper phase and adding the oil-tea camellia husk polysaccharide into an inorganic salt lower phase; (3) concentrating the ethanol upper phase in vacuum, removing salt by washing with absolute ethyl alcohol and performing freeze drying so as to obtain an oil-tea camellia husk polyphenol product; and (4) concentrating the inorganic salt lower phase with an ultrafiltration membrane and performing freeze drying so as to obtain an oil-tea camellia husk polysaccharide product. The polysaccharide and polyphenol of the oil-tea camellia husk are extracted by double-water phase technology, so that comprehensive utilization of active ingredients of the oil-tea camellia husk is realized, the production cost of the active ingredients of the oil-tea camellia husk is lowered and the activity preservation of the oil-tea camellia husk polysaccharide is facilitated.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides and polyphenols from Camellia oleifera, in particular to purifying the water extract containing polysaccharides and polyphenols from Camellia oleifera by using a two-water phase extraction method. Background technique [0002] Camellia oleifera (Camellia oleifera Abel.) is a plant of the genus Camellia in the family Theaceae, and is known as the world's four largest woody edible oil source tree species together with oil palm, coconut and olive. Camellia oleifera in my country is rich in resources, mainly distributed in Jiangxi, Hunan, Guangxi, Zhejiang, Fujian, Anhui, Guizhou and other regions. Camellia oleifera is the shell of camellia oleifera, accounting for about 2 / 3 of the total amount of camellia oleifera, and is a by-product of camellia oil production. Although my country is rich in Camellia oleifera resources, the development and utilization of Camellia oleifera is very weak. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K131/00
Inventor 徐化能顾峰庞博韬
Owner JIANGNAN UNIV