Method for extracting hydroxytyrosol

A technology of hydroxytyrosol and extraction method, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of single raw material and low yield, improve conversion rate, save production cost, and shorten conversion the effect of time

Active Publication Date: 2011-02-16
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of the product obtained in this invention is relatively high, and the original quality of the natural product is also maintained to a large extent. However, this method does not convert the oleuropein in the oli

Method used

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  • Method for extracting hydroxytyrosol

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Preparation of olive leaf extract

[0031] Use fresh olive leaves as raw materials, crush them into coarse powder, add water to decoct and extract twice (the amount of water added each time is 10 times the weight of the raw materials, and extract for 100 minutes each time), combine the decoction liquid, concentrate to the weight of the raw materials, and filter to obtain Olive Leaf Extract.

[0032]After determination, the content of iridoid glucoside oleuropein in the extract is 8%. The method of determination is (the same below): HPLC, chromatographic condition: chromatographic column (Kromasil 100-5C18); Mobile phase: acetonitrile: water=25: 75, ultraviolet detection wavelength: 285nm, column temperature: 27 ℃, flow velocity: 1mL / min).

[0033] 2. Preparation of hydroxytyrosol

[0034] 1) Take 1L of the above-mentioned extract as a raw material, add concentrated hydrochloric acid to make the pH value 1, boil for 3 hours, and enter the hydrolyzed solution into ...

Embodiment 2

[0037] 1. Preparation of Rhodiola rosea extract

[0038] Use Rhodiola rosea as the raw material, crush it to 100 mesh, add water to decoct and extract 3 times (add water 10 times the weight of the raw material for the first time, extract for 90 minutes, add water 6 times the weight of the raw material for the second time, extract for 30 minutes, and add water for the third time Extract with 6 times the weight of the raw material in water for 30 minutes), combine the decoction liquid, concentrate to the weight of the raw material, and filter to obtain the Rhodiola rosea extract.

[0039] After determination, the content of iridoid glucoside oleuropein in the Rhodiola daflora extract is 1.1%.

[0040] 2. Preparation of hydroxytyrosol

[0041] 1) Take 1L of the above-mentioned extract as a raw material, add concentrated hydrochloric acid to make the pH value 2, boil for 4 hours, adjust the pH value of the obtained hydrolyzate to 6.0 with sodium hydroxide, concentrate to the weig...

Embodiment 3

[0044] 1. Preparation of Japanese privet extract

[0045] Use the dried branches and leaves of Privet japonica as raw materials, crush them to 80 mesh, add 12 times the weight of raw materials to decoct and extract for 150 minutes, collect the decoction liquid, concentrate to the weight of raw materials, and filter to obtain Ligustrum chinensis extract liquid.

[0046] After determination, the content of iridoid glucoside oleuropein in the extract of Privet japonica is 0.8%.

[0047] 2. Preparation of hydroxytyrosol

[0048] 1) Take 1L of the above-mentioned extract as a raw material, add concentrated hydrochloric acid to make the pH value 1.5, boil for 2 hours, and enter the hydrolyzed solution into D101 macroporous adsorption resin column chromatography, collect the effluent, and use pH = 1.5 hydrochloric acid ( 3 times the volume of the resin column bed) to elute, combine the effluent and pickling solution, adjust the pH value to neutral with sodium hydroxide, concentrate...

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Abstract

The invention discloses a method for extracting hydroxytyrosol, which comprises the following steps of: preparing raw material extracting solution; taking one kind of extracting solution, adding concentrated hydrochloric acid to make the pH value thereof 0 to 2, boiling for 2 to 4 hours and hydrolyzing; allowing the hydrolyzate to pass through macroporous adsorption resin for column chromatography, eluting by using acid liquor with the pH value of 1 to 2, mixing the effluent liquid and pickling liquor, regulating the pH value to neutral, concentrating and filtering; making the filter liquor to pass through the macroporous adsorption resin for column chromatography, eluting by using 10 to 30 volume percent ethanol, collecting the eluent, concentrating, and drying to prepare the hydroxytyrosol. By acidifying the raw material extracting solution, the method realizes conversion of the hydroxytyrosol through the boiling process, greatly shortens the conversion time of the hydroxytyrosol, improves the conversion rate, integrally shortens the production cycle and saves the production cost. The hydroxytyrosol is prepared through separation and purification by the macroporous adsorption resin.

Description

technical field [0001] The invention relates to a method for extracting effective components in plants, in particular to a method for extracting hydroxytyrosol. Background technique [0002] The molecular formula of 3,4-dihydroxyphenylethanol (hydroxytyrosol) is C 8 h 10 o 3 , the structural formula is [0003] [0004] Hydroxytyrosol was originally a phenolic compound extracted from olive oil known as "liquid platinum", "beauty oil" and "Mediterranean nectar". Its content in olive oil is about 4.2mg / (100g virgin oil) and about 0.5g / (100g refined oil). Due to its excellent antioxidant properties, it is widely used in the following fields: 1. Cardiovascular drugs, which have miraculous effects on the prevention and treatment of arteriosclerosis, hypertension, heart disease, cerebral hemorrhage, etc., which are superior to similar drugs; 2. Applied in Beauty products and health care products can effectively enhance skin elasticity and moisturization, and have the effec...

Claims

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Application Information

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IPC IPC(8): C07C37/50C07C39/11C07C37/82
Inventor 孙步祥吴柏峰汤凌志陆玮易永基李霞
Owner GUILIN NATURAL INGREDIENTS CORP
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