Method for extracting polymyxin B and E from fermentation technique culture

A technology for polymyxin and culture, applied in the field of E, extraction of polymyxin B, can solve the problems of low extraction yield, high production cost, long process, etc., achieves high yield, low characteristic requirements, cost low effect

Inactive Publication Date: 2011-02-16
SHANDONG LUKANG PHARMA
View PDF11 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of this invention is to provide a method for extracting polymyxin B and E from fermentation technology culture, which is characterized by simple and efficient extraction of polymyxin B and E from fermentation broth, and solves the problem of other extraction methods. The problem of high production cost caused by high labor intensity, long process and low extraction yield in the process is an economical, green and environmentally friendly extraction method suitable for industrial production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The content of 3.0L of polymyxin B fermentation broth is 1.7g / l. First, cool the fermentation broth to 15°C; then adjust the pH to 12.5 with 4mol / L KOH solution, stir for 30 minutes and keep the temperature at 15°C; centrifuge the alkalized fermentation broth at 3000 rpm for 15 minutes, and collect the centrifuged product. After washing twice with 60ml of anhydrous salt water, adjust the pH of the supernatant to 3.0-4.0 with 1.0mol / L HCL solution to dissolve the polymyxin B precipitate; suction and filter the dissolved solution to obtain a concentrated solution plus 0.08% permanganic acid Potassium was stirred for 30 minutes, the decolorized concentrated liquid obtained was suction filtered and treated by nanofiltration. The salt-removed concentrated liquid obtained was spray-dried at an inlet temperature of 120°C, an outlet temperature of 80°C, polymyxin B hydrochloride 4.10 g, content 80.5 %.

Embodiment 2

[0023] The content of polymyxin B fermentation broth 4.0L is 1.5g / l. The temperature of the fermentation broth was first cooled to 20°C; then the pH was adjusted to 13.5 with 4mol / L NaOH solution, then stirred for 30 minutes and kept at 20°C; the alkalized fermentation broth was centrifuged at 3000 speeds for 20 minutes, and the centrifuged was collected. After washing twice with 75ml of anhydrous saline, then use 1.0mol / L of H 2 SO 4 Adjust the pH of the supernatant to 3.0-4.0 to dissolve the polymyxin B precipitate. Suction and filter the dissolved solution, add 0.07% potassium permanganate to the concentrated solution, stir for 30 minutes, suction filter the decolorized concentrated solution, nanofiltration to obtain the salt-removed concentrated solution, spray dry the inlet air temperature of 120 ℃, the outlet temperature of 75 ℃ Polymyxin B sulfate 4.76 grams, the content is 80.0%.

Embodiment 3

[0025] The content of 3.0L of polymyxin E fermentation broth is 5g / l. First, cool the fermentation broth to 20°C; then adjust the pH to 11.O with 4mol / L NaOH solution, then keep the temperature at 20°C and stir for 30 minutes; centrifuge the alkalized fermentation broth at 3000 speed for 15 minutes, and collect the centrifuged product , After washing three times with 100 anhydrous salt water, then use 1.0mol / L H 2 SO 4 Adjust the pH of the supernatant to 3.0-4.0 to dissolve the polymyxin E precipitate; filter the dissolved solution with suction to obtain a concentrated solution, add 0.1% potassium permanganate and stir for 30 minutes, filter the resulting decolorized concentrated solution with nanofiltration Obtain a demineralized concentrated solution, spray dried at an inlet temperature of 110°C, an outlet temperature of 75°C, 13 grams of polymyxin E sulfate, with a content of 81.5%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting polymyxin B and E from a fermentation technique culture, which comprises the following steps of: adjusting the pH value of the fermentation technique culture to 10.0 to 14.0 by utilizing an alkaline substance under the temperature of 0 to 25 DEG C and then stirring for 30 min; collecting fungus dregs by utilizing a solid-liquid separation technique to obtain a polymyxin B and E free alkali precipitate; adjusting the pH value of the free alkali precipitate obtained by the solid-liquid separation technique to 1.0 to 6.0 by using an acid substance or solution; after the polymyxin B and E free alkali precipitate is dissolved, carrying out solid-liquid separation to obtain the concentrated solution of polymyxin B and E salts; removing inorganic salts by using a nanofiltration technology; and finally carrying out spray drying to obtain the finished product. The method is characterized by extracting the polymyxin B and E from fermentation liquor in a convenient and high-efficiency mode, solves the problem of high production cost because of high labor strength, long working procedure and low extraction yield in the production process of other extraction methods, and has the advantages of no use of solvents, low cost, environment protection, high yield and low characteristic requirements on the fermentation liquor and equipment.

Description

Technical field: [0001] The invention relates to a method for extracting polymyxin B and E from fermentation technology culture. Background technique: [0002] Polymyxin is also called colistin sulfate, its English common name: Polymyxin BSulfate, molecular formula: C 56 H 98 N 16 O 13 H 2 SO 4 , Molecular weight: 1301.56. [0003] Polymyxin is the general term for a family of basic peptide antibiotics, which is produced by Bacillus polymyxa (Bacillus polymyxa), and is composed of a variety of amino acids and fatty acids. Polymyxins produced by different strains are The difference between amino acids and fatty acids is divided into polymyxin A, B, C, D, E, and M. Polymyxin B (polymyxin B) is divided into B1 and B2, polymyxin E (polymyxin E, colistin,) is divided into E1 and E2, polymyxin M is also known as polymyxin A, polymyxin Both polymyxin B and polymyxin E have similar pharmacological effects and are widely used. Polymyxin B is mostly used for human medicine, and polymyxin E...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/62C07K1/14
Inventor 左良成常晓菲张利强于雪梅
Owner SHANDONG LUKANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap