Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of multi-level structure immobilized enzyme

A technology for immobilizing enzymes and preparation steps, applied in the field of bioengineering, can solve the problems of high enzyme activity and stable immobilization, and achieve good activity and operational stability, high enzyme load, and prevent the loss of enzymes. Effect

Inactive Publication Date: 2012-05-30
BEIJING UNIV OF CHEM TECH
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing immobilized enzymes with a multi-level structure, which solves the problem that it is difficult to have both high activity and stable immobilization of enzymes in the immobilized enzyme system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of alumina-silicon oxide composite film

[0019] Dissolve 1 g of polyvinyl ether-polypropylene ether-polyvinyl ether block copolymer (P123) in a mixture of 5 g of ethanol and 1.2 g of 0.2 mol / L hydrochloric acid at room temperature, add 0.03 g of toluene, and stir for 2 hours. 2.08 g of ethyl orthosilicate was added dropwise into the solution, and stirred for 1 hour to obtain a silicon oxide precursor solution. An anodic aluminum oxide film with a diameter of 47mm and a pore size of 200nm was immersed in the silicon oxide precursor solution, and volatilized under vacuum for 1 hour. The aluminum oxide film was taken out and naturally crystallized for 1 day. 180mL ethanol was refluxed three times for 24 hours to extract the block copolymer P123. Dry at room temperature.

[0020] (2) Filtration and adsorption of hemoglobin

[0021] Take 5 mL of 0.1 mol / L disodium hydrogen phosphate and 90 mL of 0.1 mol / L potassium dihydrogen phosphate solution to prepar...

Embodiment 2

[0030] (1) Preparation of alumina-silicon oxide composite film

[0031] Dissolve 1 g of polyvinyl ether-polypropylene ether-polyvinyl ether block copolymer (P123) in a mixture of 5 g of ethanol and 1 g of 0.2 mol / L hydrochloric acid at room temperature, add 0.15 g of toluene, and stir for 2 hours. 2.08 g of ethyl orthosilicate was added dropwise into the solution, and stirred for 1 hour to obtain a silicon oxide precursor solution. An anodic aluminum oxide film with a diameter of 47mm and a pore size of 200nm was immersed in the silicon oxide precursor solution, and volatilized under vacuum for 1 hour. The aluminum oxide film was taken out and naturally crystallized for 1 day. 180mL ethanol was refluxed three times for 24 hours to extract the block copolymer P123. Dry at room temperature.

[0032] (2) Filtration and adsorption of myoglobin

[0033]Take 10 mL of 0.1 mol / L disodium hydrogen phosphate and 90 mL of 0.1 mol / L potassium dihydrogen phosphate solution to prepare a...

Embodiment 3

[0042] (1) Preparation of alumina-silicon oxide composite film

[0043] Dissolve 0.687g of polyvinyl ether-polypropylene ether-polyvinyl ether block copolymer (P123) in a mixture of 4.8g of ethanol and 1.2g of 0.2mol / L hydrochloric acid at room temperature, add 0.01g of toluene, and stir for 2 hours . 2.08 g of ethyl orthosilicate was added dropwise into the solution, and stirred for 1 hour to obtain a silicon oxide precursor solution. An anodic aluminum oxide film with a diameter of 47mm and a pore size of 200nm was immersed in the silicon oxide precursor solution, and volatilized under vacuum for 1 hour. The aluminum oxide film was taken out and naturally crystallized for 1 day. 180mL ethanol was refluxed three times for 24 hours to extract the block copolymer P123. Dry at room temperature.

[0044] (2) Filtration and adsorption of trypsin

[0045] Take 100mL of 0.1mol / L Tris and 11.4mL of 0.1mol / L hydrochloric acid solution to prepare a pH 9 buffer solution to prepare ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of multi-level structure immobilized enzyme, belonging to the technical field of biological engineering. The preparation method thereof includes: (1) alumina-silicon oxide composite membrane in mesoporous structure is prepared; (2) filtering and absorption are carried out, and enzyme is fixed on the alumina-silicon oxide composite membrane; (3) a mesoporous nano particle membrane is modified on the surface of the alumina-silicon oxide composite membrane, thus forming the multi-level structure immobilized enzyme. The method has mild preparation conditions, enzyme and carrier are absorbed by virtue of physical action, and enzyme can maintain high activity; mesoporous nano particle membrane prevents drain of enzyme without influencing substrate pass, so that immobilized enzyme has good operation stability; and meanwhile the preparation method can realize continuous operation of immobilized enzyme and direct separation of products.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for preparing immobilized enzymes with a multi-level structure. In the method, the enzyme is fixed in the mesopore of the aluminum oxide-silicon oxide composite membrane, and the surface of the aluminum oxide-silicon oxide composite membrane is further modified with the mesoporous nanoparticle membrane to prevent the loss of the enzyme. The preparation method can obtain the immobilized enzyme with high activity and high stability, and has universal applicability. Background technique [0002] Immobilized enzymes have important applications in food, medicine, chemical industry and biosensor manufacturing. After more than 50 years of research and development, enzyme immobilization technology has made great progress, and a variety of immobilization methods and carrier materials with various performances have been developed. It can not only stabilize enzymes and ach...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14
Inventor 何静卢珊
Owner BEIJING UNIV OF CHEM TECH