Laxogenin and derivative thereof-containing plant growth regulator and application thereof
A plant growth regulator, laxogenin technology, applied in the field of development and utilization of plant growth regulators, can solve the problems that the actual application of laxogenin and its derivatives has not been reported, and achieve the improvement of agronomic properties, experimental The effect of fewer steps and less technical difficulty
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Embodiment 1
[0058] Example 1 Laxogenin (La, R=H) and its derivative A (LG, R=β-D-glu-) on the growth regulation of Pinus tabulaeformis seeds
[0059] 1. Materials and methods
[0060] 1.1 Test materials
[0061] 1.1.1 Species to be tested
[0062] Pinus tabulaeformis seeds: collected in Luliang, Shanxi, China in October 2008, with an initial moisture content of 9.05% and a thousand-grain weight of 48.78g. Initial treatment: Wash the seeds, remove the bad seeds, rotten seeds and sundries floating on the water surface, and rinse with clean water. Lay flat in a well-ventilated place to dry quickly, and store in a 4°C refrigerator for later use.
[0063] 1.1.2 Reagents
[0064] Laxogenin (La, R=H) and Laxogenin A (LG, R=β-D-glu-) self-made; control: brassinosteroid (BR, Chengdu New Chaoyang Biochemical Co., Ltd.)
[0065] 1.2 Research Methods
[0066] 1.2.1 Seed treatment
[0067] By adding water regularly and quantitatively to control the water absorption speed and water absorption am...
Embodiment 2
[0137] Example 2 Laxoside B (LGB, R=α-L-Ara-(1→6)-β-D-glu-) Regulatory Effect on the Growth of Pinus tabulaeformis Seedlings
[0138] 1. Materials and methods
[0139] 1.1 Test materials
[0140] 1.1.1 Species to be tested
[0141] Pinus tabulaeformis seeds: collected in Luliang, Shanxi, China in October 2008, with an initial moisture content of 9.05% and a thousand-grain weight of 48.78g. Initial treatment: Wash the seeds, remove the bad seeds, rotten seeds and sundries floating on the water surface, and rinse with clean water. Lay flat under a fan or in a well-ventilated place to dry quickly, and store in a 4°C refrigerator for later use.
[0142] 1.1.2 Reagents
[0143] Laxoside B (LGB, R=α-L-Ara-(1→6)-β-D-glu-) self-made, polyethylene glycol (Tianjin Kemiou Chemical Reagent Development Center), brassinolide ( BR, Chengdu New Chaoyang Biochemical Co., Ltd.),
[0144] 1.2 Research Methods
[0145] 1.2.1 Seed treatment
[0146] By adding water regularly and quantitati...
Embodiment 3
[0227] Example 3 Laxogenin and its derivative C (R=I) on the growth regulation of Chinese seabuckthorn seeds
[0228] 1 Materials and methods
[0229] 1.1 Test material
[0230] Instruments and equipment: Visible ultraviolet spectrophotometer UV-1700 (Shimadzu Japan), Li-6400 portable light and measurement system (Licor America), initiator (diameter 9cm, height 15cm flat-bottom inoculation bottle with cover), nutrition bowl ( Upper diameter 15cm, lower diameter 10cm, height 25cm)
[0231] Test reagents: thiobarbituric acid, nitro blue tetrazolium, enone, ninhydrin and Coomassie brilliant blue.
[0232] Test materials: Hippophae rhamnoides seeds collected in Inner Mongolia, China in October 2007, with a thousand-grain weight of 9.1609g and an initial water content of 9.03%.
[0233]Laxogenin and Laxogenin C (R=I): self-made by the School of Life Sciences, Northwest A&F University, China.
[0234] Brassinolide: Chengdu New Chaoyang Biochemical Co., Ltd.
[0235] In this tes...
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