Method for recovering glucomannan and protein in konjac flying powder by enzyme process

A technology of konjac flying powder and glucomannan, which is applied in animal feed, animal husbandry, application, etc., can solve problems such as technical difficulties in protein and konjac glucomannan recovery, improve resource utilization efficiency, reduce pollution, and facilitate The effect of the operation

Inactive Publication Date: 2011-04-20
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of starch and high-viscosity glucomannan will cause konjac fly powder to form a viscous, gel-like solution after mixing with water, making it difficult to separate protein, starch and other components by traditional protein extraction methods. Mannan recovery presents technical difficulties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Ingredients: Weigh 5g of konjac powder, add it into a beaker with 200 mL of water, and stir it fully with an electric stirrer to obtain an aqueous solution of konjac powder;

[0031] Liquefaction: Heat the aqueous solution of konjac powder in a water bath to 45°C, adjust the pH to 4.0, add fungal α-amylase, the amount of fungal α-amylase is 10 U / g konjac powder, and liquefy for 2 hours. Until the starch is fully degraded (judged by iodine-potassium iodide reagent: add iodine-potassium iodide reagent to the solution, if no blue color appears in the solution, it indicates that the starch has been fully degraded), the solution viscosity decreases significantly, and the reducing sugar content increases;

[0032] Saccharification: adjust the temperature of the liquefied solution to 40°C, adjust the pH to 3.0, add amyloglucosidase, the amount of amyloglucosidase is 10 U / g konjac powder, stir evenly, and continue to react for 24 hours;

[0033] Centrifugation: adjust the pH of...

Embodiment 2

[0038] Ingredients: Weigh 5g of konjac flying powder, add it to a beaker with 1000 mL of water, and use an electric stirrer to fully stir it evenly to obtain an aqueous solution of konjac flying powder;

[0039] Liquefaction: Heat the aqueous solution of konjac powder to 65°C in a water bath, adjust the pH to 6.6, add fungal α-amylase, the amount of fungal α-amylase is 500 U / g konjac powder, react for 2 hours, until The starch is fully degraded (judged by iodine-potassium iodide solution), the viscosity of the solution is significantly reduced, and the content of reducing sugar is increased;

[0040] Saccharification: adjust the temperature of the liquefied solution to 60°C, adjust the pH to 5.5, add amyloglucosidase, the amount of amyloglucosidase is 500 U / g konjac fly powder, stir evenly, and continue to react for 3 hours;

[0041] Centrifugation: adjust the pH of the saccharified solution to 5, set the centrifugation parameters as: centrifugation rate 5000 rpm / min, centrifu...

Embodiment 3

[0046] Ingredients: Weigh 5g of konjac flying powder, add it to a beaker with 400 mL of water, and use an electric stirrer to fully stir it evenly to obtain an aqueous solution of konjac flying powder;

[0047] Liquefaction: Heat the aqueous solution of konjac powder in a water bath to 50°C, adjust the pH to 5.0, add medium-temperature α-amylase, the amount of medium-temperature α-amylase is 10 U / g konjac powder, react for 2 hours, until The starch is fully degraded (judged by iodine-potassium iodide solution), the viscosity of the solution is significantly reduced, and the content of reducing sugar is increased;

[0048] Saccharification: adjust the temperature of the liquefied solution to 40°C, adjust the pH to 3.0, add amyloglucosidase, the amount of amyloglucosidase is 10 U / g konjac powder, stir evenly, and continue to react for 24 hours;

[0049] Centrifugation: adjust the pH of the saccharified solution to 3.5, set the centrifugation parameters as follows: centrifugation...

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Abstract

The invention relates to a method for recovering glucomannan and protein in konjac flying powder by the enzyme process, which comprises the following process steps: (1) liquification: adding alpha-amylase into water solution of the konjac flying powder and liquifying till starch is fully degraded; (2) saccharification: adding amyloglucosidase into solution in the step (1) for saccharification; (3) centrifugation: regulating the pH of the solution in the step (2) to 3.5-5.0, centrifugating and getting precipitate; (4) acid washing: adding water into the precipitate in the step (3), regulating the pH of the solution to acidic condition, stirring, centrifugating, and getting the precipitate; and (5) drying: regulating the pH of the precipitate after the acid washing of the step (4) to be neutral, drying and finally getting a final extract. The process flow is simple, the operation is easy, the content of the glucomannan and crude protein in the got extract is high, and the extract can be used as a high-quality feed or a protein raw material and the like; and a stinking matter of trimethylamine and an antinutritional factor of tannin can be effectively removed.

Description

technical field [0001] The invention relates to a method for recovering glucomannan and protein in konjac powder by enzymatic method. Background technique [0002] Konjac fly powder (Konjac Fly Powder, KFP) is a by-product produced during the dry processing of glucomannan, accounting for 36-45% of the mass of konjac powder before processing. It contains about 50% of total sugar (mainly starch and glucomannan), about 20% of protein, about 0.5% of fat, about 2.5% of cellulose and about 7% of minerals and other nutrients, as well as nerve Amides, flavonoids, alkaloids and other bioactive substances. According to a conservative estimate, in 2004 the national konjac planting area has reached more than 24,000 hectares, and there are more than 100 konjac deep-processing enterprises nationwide. Only about 85% (about 80,000 tons) of konjac flying powder is not utilized in Hubei Province every year, and it is easy to cause great environmental pollution. However, konjac powder conta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L5/02C12P19/08C12P21/02A23K1/14A23J1/00A23K10/37
CPCY02P60/87
Inventor 王程刘慧
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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