Microbial bait additive and preparation method thereof

A technology of additives and microorganisms, applied in the field of microbial bait additives and its preparation, can solve the problems of growth and the influence of digestive enzyme activity, which are rarely reported, and achieve the effect of ensuring the number of effective viable bacteria, good effect and good reproducibility

Inactive Publication Date: 2011-04-27
夏学德 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial feed additives for Penaeus vannamei are widely used in aquaculture, but their effects on the growth and digestive enzyme activities of Penaeus vannamei are rarely reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, microbial bait additive, comprises Bacillus cereus culture 15%, Bacillus subtilis culture 15%, Bacillus coagulans 10%, Lactobacillus plantarum culture 25%, Lactobacillus acidophilus culture 25% and swamp Rhodopseudomonas culture 10%.

[0048] Prepare as follows:

[0049] a, preparation of Bacillus cereus culture: insert the Bacillus cereus seed solution into the sterilized medium, the medium contains 5% bean sprout juice, 0.2% glucose and 1% peptone by weight, The balance is water as a solvent, the inoculation amount is 0.3%, and after aerobic fermentation at a temperature of 32° C. and a pH value of 7.2 for 24 hours, the tank is placed to obtain a Bacillus cereus fermentation culture;

[0050] B, Bacillus subtilis culture: Bacillus subtilis seed liquid inserts in the substratum after the sterilization, and substratum contains the bean sprouts juice that is 5% by weight, the glucose of 0.2% and the peptone of 1%, the surplus is as The water of the solven...

Embodiment 2

[0056] Embodiment 2, after centrifuging each strain culture of embodiment 1, mix with calcium carbonate carrier in a weight ratio of 1:3, and dry at 45°C-75°C to prepare the above-mentioned bacterial culture Solid bacterial powder, the mixing and ratio of various solid bacterial powders in the final product are: Bacillus cereus 1%, Bacillus subtilis 1%, Lactobacillus plantarum 2%, Lactobacillus acidophilus 2%, Bacillus coagulans 1%, marsh 1% rhodopseudomonas, the carrier is seaweed powder, mixed and packaged to make solid microbial bait additive.

Embodiment 3

[0057] Embodiment 3, microbial bait additive, comprises Bacillus cereus strain and Bacillus subtilis mixed culture 30%, Bacillus coagulans culture 10%, Lactobacillus plantarum and Lactobacillus acidophilus mixed culture 50% and swamp red pseudo single Bacteria culture 10%.

[0058] Prepare each mixed strain fermented liquid as follows:

[0059] a. Prepare the mixed culture of Bacillus cereus strain and Bacillus subtilis: insert the seed liquid of Bacillus cereus and Bacillus subtilis in a ratio of 1:1 by weight into the sterilized medium, the medium contains 5% bean sprouts juice, 0.5% glucose and 1% peptone, the balance is water as a solvent, the inoculum size is 0.3%, after aerobic fermentation at a temperature of 32°C and a pH value of 7.2 for 24 hours, put tank to obtain a mixed fermentation culture of spores;

[0060] b. Preparation of mixed culture of Lactobacillus plantarum and Lactobacillus acidophilus: insert the seed solution of Lactobacillus plantarum and Lactobac...

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PUM

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Abstract

The invention discloses a microbial bait additive, which comprises 10 to 40 percent of bacillus cereus culture, 10 to 40 percent of bacillus subtilis culture, 5 to 20 percent of bacillus coagulans, 10 to 40 percent of lactobacillus plantarum culture, 10 to 40 percent of lactobacillus acidophilus culture and 5 to 20 percent of rhodopseudomonas palustris culture. The microbial bait additive can improve the stress resistance of penaeus vannamei boone, has the effects of increasing young shrimp yield and improving aquaculture water quality, and can fully utilize the growth characteristics of each strain to reduce fermentation times and obtain the largest amount of beneficial microorganisms so as to reduce production cost by single strain batch fermentation and multiple strain mixed fermentation technology.

Description

technical field [0001] The invention relates to a microbial bait additive and a preparation method thereof. Background technique [0002] In recent years, my country's vannamei aquaculture industry has been developing continuously, but with the expansion of aquaculture scale, the aquaculture environment has deteriorated day by day, and there have been some problems such as decreased immunity of adult shrimp, slow growth of juvenile shrimp, and low survival rate. Seriously restricted the development of vannamei aquaculture. Penaeus vannamei microbial bait additive, as a green additive, has developed very rapidly in recent years. It can not only improve the intestinal micro-ecological environment of animals and secrete digestive enzymes, improve the conversion rate of bait, prevent diseases, and promote growth; it can also reduce ammonia nitrogen in water. , nitrite and H 2 The content of S and other substances also plays a very critical role in improving water quality. Micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/18C12N1/20C12R1/125C12R1/085C12R1/25C12R1/23A23K10/18A23K50/80
CPCY02A40/818
Inventor 夏学德袁杰利唐玉光曲嫣红姜成斌
Owner 夏学德
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