Microbial live bacterium agent, preparation method and application thereof

A technology of live bacteria preparation and microorganism, applied in the field of microorganism preparation, can solve problems such as being unfavorable to human health, unable to guarantee vitality, and reducing the number of live bacteria, and achieve the effects of comprehensive health care efficacy, low cost and wide applicability

Active Publication Date: 2011-04-27
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned microbial preparations use milk powder, glucose, etc. as microbial freeze-drying protective agents, which are costly and contain animal-derived ingredients, which are not conducive to human health and are not suitable for specific groups of people.
In addition, although it can protect microorganisms to a certain extent, the number of viable bacteria decreases rapidly during a longer storage period, and its vitality cannot be guaranteed after long-term storage

Method used

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  • Microbial live bacterium agent, preparation method and application thereof
  • Microbial live bacterium agent, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of live lactic acid bacteria preparation:

[0025] Take the prepared bacterial cellulose membrane (also commercially available), cut it into 5 × 5mm cubic particles, sterilize and set aside;

[0026] Take 10g of peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, 801ml of Tween, 2g of diammonium hydrogen citrate, 5g of sodium acetate, 2g of dipotassium hydrogen phosphate, MnSO 4 ·7H 2 O0.05g, MgSO 4 ·7H 2 O 0.2g, calcium carbonate 20g, agar 20g, distilled water 1L to prepare the culture medium, adjust the pH to 6.8 with NaOH, and sterilize.

[0027] Add the above-mentioned bacterial cellulose particles into the culture medium in a volume ratio of 30:100, and stir and mix;

[0028] Inoculate Lactobacillus bulgaricus and culture at 37°C for 12 hours;

[0029] Remove bacterial cellulose particles under aseptic conditions and wash with sterile distilled water;

[0030] Use a freeze dryer to freeze-dry for 12 hours at -40 ° C and a vacuum of ...

Embodiment 2

[0033] Preparation of live bifidobacterium preparations:

[0034] Take the prepared bacterial cellulose membrane, cut it into 3 × 3mm cubic particles, sterilize and set aside;

[0035] Get commercially available bifidobacterium BS culture medium (Shanghai Hufeng Biotechnology Co., Ltd.), and sterilize;

[0036] Add the above-mentioned bacterial cellulose particles into the culture medium in a volume ratio of 50:100, and stir and mix;

[0037] Inoculate Bifidobacterium bifidum, culture at 37°C for 10 hours;

[0038] Remove bacterial cellulose particles under aseptic conditions and wash with sterile distilled water;

[0039] Pre-cool at -10°C for 15 minutes;

[0040] Use a freeze dryer to freeze-dry for 8 hours at -80°C and a vacuum of 40Pa;

[0041] Pulverize and pack aseptically to obtain a powdered live bifidobacterium preparation.

Embodiment 3

[0043] Preparation of live yeast preparations:

[0044] Take the prepared bacterial cellulose membrane, cut it into 8×8mm cubic particles, sterilize and set aside;

[0045] Get commercially available yeast YPD medium (Shanghai Jiemei Gene Pharmaceutical Technology Co., Ltd.), and sterilize;

[0046] Add the above-mentioned bacterial cellulose particles into the culture medium in a volume ratio of 70:100, and stir and mix;

[0047] Inoculate Saccharomyces cerevisiae and culture at 27°C for 5 hours;

[0048] Remove bacterial cellulose particles under aseptic conditions and wash with sterile distilled water;

[0049] Pre-cool at -20°C for 8 minutes;

[0050]Use a freeze dryer to freeze-dry for 7 hours at -100°C and a vacuum of 60Pa;

[0051] Pulverize and pack aseptically to obtain a powdered live yeast preparation.

[0052] Detection:

[0053] The live bacterial preparations in Examples 1-3 were respectively taken and stored at 4°C. Determination of the number of live bac...

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Abstract

The invention belongs to the field of microbial agents, and in particular relates to a microbial live bacterium agent and a preparation method thereof. The microbial live bacterium agent is prepared by a microbial agent preparation process and by taking bacterial cellulose as a freeze-drying protective agent and carrier; the preparation method for the microbial live bacterium agent comprises the following steps of: preparing the bacterial cellulose into particles, adding the particles into a microbiological culture medium, uniformly stirring and mixing the particles, and then inoculating microorganisms and culturing; separating the bacterial cellulose particles under the aseptic condition, and washing the bacterial cellulose particles with sterile distilled water; and freezing and drying the bacterial cellulose particles, then crushing the particles into powder, and finally packaging the powder under the aseptic condition. The microbial live bacterium agent provided by the invention can effectively protect the activity of lactic acid bacteria; and the microorganisms still have high activity. The microbial live bacterium agent has the characteristics of convenience for use, low cost, wide applicability and the like, can be widely applied in food industry, and has more complete healthcare effects.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and in particular relates to a live microbial preparation, a preparation method thereof, and an application in the food field, including a composite live microbial preparation containing the live microbial preparation. Background technique [0002] Microorganisms are tiny, but they are closely related to human life. Many microorganisms are also used in the production of food, such as brewing wine, making vinegar, producing bread, dairy products and beverages. At present, the utilization of food microorganisms can be roughly divided into two categories, one is to use the fermentation of microorganisms to produce food by fermentation, such as brewing beer with beer yeast, and the other is to use microorganisms to improve the micro-ecological balance of the host and play a beneficial role Features, directly added to various foods, such as the use of probiotics. When using microorganisms, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12
Inventor 钟春燕
Owner 钟春燕
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