Method for quickly and simply analyzing microbial community structure of yeast for traditional brewage
A microbial community, a simple technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, analysis materials, etc., to achieve the effect of effective analysis methods, solving analysis errors, and simple operation
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[0012] Add 3.2ml of phosphate buffer, 4ml of chloroform and 8ml of methanol to 3g of Daqu sample successively, shake in the dark at room temperature at 250r / min for 2h. Add 4.8ml of buffer solution and 6ml of chloroform successively, shake vigorously and mix well, and let stand overnight. The chloroform phase (lower layer) was transferred to a new test tube and blown dry with nitrogen. The extracted fatty acid was dissolved in chloroform, transferred to a silica gel column, and eluted with 10ml chloroform, 10ml acetone, and 10ml anhydrous methanol respectively, and the methanol eluate was collected and blown dry with nitrogen. Add 1ml of toluene:methanol ((1:1) v / v) to the dried solid component, 1ml of 0.2mol·l -1 Potassium hydroxide-methanol, 37°C water bath for 15min, after cooling, add 2ml ultrapure water and 0.3ml acetic acid solution (1mol / L), extract FAME with n-hexane (4ml), take the organic phase, blow dry with nitrogen, add internal standard FAME samples were analyz...
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