Interference imaging method and system thereof for real-time detection of interaction of biological molecules

Abstract

The invention relates to an interference imaging method and a system thereof for real-time detection of interaction of biological molecules, belonging to the field of biotechnologies; and the method comprises the following steps: light emitted by a light source is subject to collimation, polarization and beam expansion, and then is radiated into a sensing unit; the light reflected by the sensing unit passes through a half-wave plate, an imaging lens and a polarizing beam splitter prism, and then is divided into two paths of light, which are the transmitted light and the reflected light in theorthometric propagation directions, and further imaged on two CCDs (charge coupled devices); a computer is used for collecting two frames of interference images in real time, and an information diagram of refractive index distribution is obtained through resolution; and the sensing unit is measured continuously so as to obtain a real-time information diagram of the distribution of a series of refractive indexes along with the time change. The system comprises an incident arm for generating collimated light, the biological sensing unit for receiving the collimated light and exciting surface plasmon resonance and a collecting and processing unit; and by using the method and the system, protein chips can be detected in a high throughput, high precision, real-time and unmarked manner, and interaction information between protein molecules and between proteins and other biological molecules can be further obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to high-throughput, high-precision, label-free, real-time sensing protein-protein, protein-DNA, protein-effector, antigen-antibody, ligand-receptor, drug- A method for the interaction of biomolecules such as targets and a protein chip detection system thereof. Background technique [0002] Life science has entered the era of proteomics research. Proteomics is the study of a complete set of proteins expressed by a genome, including all proteins expressed by a cell or even an organism. It must be on a large-scale level to study the characteristics of proteins, including protein expression levels, post-translational modifications, and protein-protein interactions; at the same time, it also focuses on studying all protein types involved in specific physiological or pathological states and their relationship The relationship between the surrounding environment (molecules). In t...

AI Technical Summary

Problems solved by technology

However, when a high voltage is applied to the electro-optic crystal 107, the inverse piezoelectric effect will appear, resulting in micro-scale changes in the structure of the electro-optic crystal, causing a small shift in the optical path, that is, the position of the spot moves, and directly produces measurement errors. meet high precision requirements

This is an inherent defect of electro-optic crystals that cannot be remedied

Not only that, this method is time-sharing instead of collecting 5 frames of images at the same time. Although the interval is short, it can only be regarded as "quasi-real-time", not real-time in the true sense

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