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Hydrogen peroxide electrochemical sensor and manufacturing method thereof

A chemical sensor, hydropower technology, applied in the direction of material electrochemical variables, etc., can solve the problems of difficult electron transfer, difficult exposure, poor chemical stability and thermal stability of enzyme molecules, etc., to achieve rapid electrocatalytic performance and electrochemical signal improvement , the effect of good response

Inactive Publication Date: 2013-05-29
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are still many shortcomings in the application of such enzyme-based bioelectrochemical sensors in the field of detection, mainly reflected in: (1) poor chemical stability and thermal stability of enzyme molecules; (2) active sites of enzyme molecules Usually surrounded by peptide chains, the interior is not easily exposed, so it is difficult for electrons to transfer between the enzyme and the electrode

Method used

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  • Hydrogen peroxide electrochemical sensor and manufacturing method thereof
  • Hydrogen peroxide electrochemical sensor and manufacturing method thereof
  • Hydrogen peroxide electrochemical sensor and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. The preparation mass fraction is the naphthol green solution A of 1g / L;

[0029] 2. Preparation of nitrate hydrotalcite by ion exchange method:

[0030] a. Add 0.01mol of solid Co(NO 3 ) 2 ·6H 2 O and 0.005 mol of solid Al(NO 3 ) 3 9H 2 O and 0.06mol urea were dissolved in 50mL of deionized water, and crystallized in a 90ml polytetrafluoroethylene pressure reaction vessel at 120°C for 48 hours, then centrifugally washed with deionized water until the pH was about 7,50 Dry at ℃ for 24 hours to obtain carbonate intercalated hydrotalcite powder;

[0031] b. Take 0.3 g of the above-mentioned carbonate intercalation hydrotalcite powder and solid NaNO 3 63.75g dissolved in 300mL to remove CO 2 After uniform dispersion in deionized water, add 0.09mL of concentrated nitric acid and stir at 20°C under a nitrogen atmosphere to carry out ion exchange reaction for 24 hours and then use 2 Centrifugal washing with deionized hot water until the pH is about 7, and vacuum dr...

Embodiment 2

[0037] 1. The preparation mass fraction is the naphthol green solution A of 1g / L;

[0038] 2. With embodiment 1;

[0039] 3. Obtain clear transparent colloidal solution B with embodiment 1;;

[0040] 3. The glassy carbon electrode sheet is made of 0.05μm Al 2 o 3 The powder is ground into a mirror surface, cleaned with ultrapure water, ultrasonicated in concentrated nitric acid, absolute ethanol, and ultrapure water for 30 seconds, and the cleaned glassy carbon electrode sheet is soaked in solution A for 10 minutes, and fully cleaned with deionized water Finally, placed in solution B, soaked for 10 minutes and fully cleaned to obtain a cycle of naphthol green and hydrotalcite composite film;

[0041] 4. Repeat steps 3 and 12 times to obtain a multilayer composite film of naphthol green and hydrotalcite, and vacuum-dry to obtain a hydrogen peroxide electrochemical sensor.

[0042] Utilize the electrochemical workstation with silver / silver chloride as the reference electrode...

Embodiment 3

[0044] 1. The preparation mass fraction is the naphthol green solution A of 2g / L;

[0045] 2. With embodiment 1;

[0046] 3. Obtain clear transparent colloidal solution B with embodiment 1;;

[0047] 3. The glassy carbon electrode sheet is made of 0.04μm Al 2 o 3 The powder is ground into a mirror surface, cleaned with ultrapure water, ultrasonicated in concentrated nitric acid, absolute ethanol, and ultrapure water for 20 seconds, and the cleaned glassy carbon electrode sheet is soaked in solution A for 12 minutes, and fully cleaned with deionized water Finally, placed in solution B, soaked for 12 minutes and fully cleaned to obtain a cycle of naphthol green and hydrotalcite composite film;

[0048] 4. Repeat steps 3 and 15 times to obtain a multi-layer composite film of naphthol green and hydrotalcite, and vacuum-dry to obtain a hydrogen peroxide electrochemical sensor.

[0049] Use silver / silver chloride as a reference electrode, platinum wire as a counter electrode, th...

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Abstract

The invention discloses a hydrogen peroxide electrochemical sensor decorated by naphthol green and multiple layers of houghite composite films and a production method, belonging to the technical field of electrochemical sensor manufacturing. The production method of the hydrogen peroxide electrochemical sensor comprises the following steps of: preparing a houghite nanosheet colloidal solution anda naphthol green solution; and alternately assembling the treated glassy carbon electrode piece with negative charge in the two solutions to obtain the hydrogen peroxide electrochemical sensor decorated by the naphthol green and multiple layers of houghite composite films. The invention has the advantages that hydrogen peroxide is subjected to electrocatalysis by a houghite nanosheet as an electroactive substance through the self oxidation-reduction reaction; and by using a layer-by-layer self-assembling method, active components are combined in the molecular level and the films are controllable in the nanometer level, and obtained high-orientation films ensures that the sensor has favorable current response.

Description

technical field [0001] The invention belongs to the technical field of preparation of electrochemical sensors, in particular to a hydrogen peroxide electrochemical sensor modified by naphthol green and hydrotalcite multilayer composite films and a preparation method thereof. Background technique [0002] In recent years, due to its high selectivity, high sensitivity, fast analysis, convenient operation and other advantages, electrochemical biosensors have been widely demanded in the fields of biomedicine, environmental monitoring, food and pharmaceutical industries. In 1962, Clark first proposed the design principle of developing a glucose enzyme biosensor. On this basis, UPdike and Hicks successfully developed a glucose oxidase sensor in 1967, which was used to quantitatively detect the content of glucose in serum. Since then, research on biosensors based on enzyme electrodes has developed rapidly. At present, there are still many shortcomings in the application of such en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 卫敏孔祥贵段雪
Owner BEIJING UNIV OF CHEM TECH
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