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Method for extracting yellow mealworm active proteins by using alkali-enzyme method

An active protein and enzymatic extraction technology, applied in the field of deep processing of Tenebrio molitor protein, can solve the problems of less development and utilization, waste of nutrient resources, lack of systematic research, etc., and achieves short heating time, less investment equipment, and easy control of product quality. Effect

Inactive Publication Date: 2012-12-05
CHANGSHA SAIBANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, due to the lack of systematic research on it, it has been less developed and utilized, and a considerable part of it has been used as feed, causing a large amount of nutritional resources to be wasted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take 100 g of fresh Tenebrio molitor larvae and put them into a freeze dryer at -15°C for 10 hours, then dry them continuously for 24 hours under the condition of vacuum degree of 100 Pa and cold trap temperature of -50°C to obtain dried Tenebrio molitor larvae, which are ready for use. Use petroleum ether with a relative density of 0.64 to 0.66 as the leaching solvent, add petroleum ether at the ratio of 1g insect powder to 3ml petroleum ether, and perform secondary extraction. The extraction temperature is 60°C, and the extraction time is 2 hours each time to obtain Tenebrio molitor. Skimmed dry powder. Freeze-dried and degreased Tenebrio molitor dry powder was pre-extracted at 45°C for 1 hour with 0.08mol / L NaOH solution at a ratio of 6ml NaOH solution per gram of insect powder. Then, adjust the pH value to 7.0, at 55°C, add 2.0g of neutral protease and papain, the mass ratio of neutral protease and papain is 1:1, after the extraction time of 120min, heat up to 90°C,...

Embodiment 2

[0016] Take 100g of fresh Tenebrio molitor larvae and pre-freeze them at -15°C for 15 hours before putting them into a freeze dryer, and then dry them continuously for 24 hours under the conditions of a vacuum of 100Pa and a cold trap temperature of -50°C to obtain dry Tenebrio molitors for later use. Use petroleum ether as the leaching solvent, add 500ml petroleum ether (relative density is 0.64-0.66, analytically pure) for secondary extraction, the leaching temperature is 80°C, and each leaching time is 3h, to obtain the degreasing dry powder of Tenebrio molitor. Freeze-dried and degreased Tenebrio molitor dry powder was pre-extracted at 50°C for 2 hours with 0.10mol / L NaOH solution at a ratio of 8ml NaOH solution per gram of insect powder. Then, adjust the pH value to 7.5, at 60°C, add 3.0g of neutral protease and papain, the mass ratio of neutral protease and papain is 1: 1.5, after the extraction time of 150min, heat up to 90°C, and keep warm for 5 ~10min to inactivate th...

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Abstract

The invention provides a method for extracting yellow mealworm active proteins by using an alkali-enzyme method, comprising the following steps: drying fresh yellow mealworm larvae to obtain dried yellow mealworm larvae of which the moisture content is lower than 5%, and smashing the dried yellow mealworm larvae; carrying out secondary digestion by taking petroleum ether of which the relative density is 0.64-0.66 as an extraction solvent; carrying out digestion with a 0.08-0.10mol / L NaOH solution; regulating the pH value to 7.0-7.5, carrying out enzymatically hydrolyzed extraction at a temperature of 55-60 DEG C by complex enzyme which is composed of neutral protease and papain at the mass ratio of 1:1-1:1.5; destroying enzyme; and centrifuging and dialyzing to obtain yellow mealworm protein powder. The yellow mealworm protein powder has the advantages of light yellow color and luster, beauty, fragrant odor as well as fine and smooth taste; and the protein content of the yellow mealworm protein powder reaches 94.5% and the extraction efficiency of the yellow mealworm protein powder reaches 75%.

Description

Technical field: [0001] The invention relates to the field of food, in particular to a deep processing technology for extracting Tenebrio molitor protein which can be used in food production by using an alkaline enzyme method. Background technique: [0002] Tenebrio molitor (Tenebrio molitor.Linne), also known as: yellow powder armor, commonly known as bread worm, belongs to Insecta, Coleoptera, Pedidae, Tenebrio genus, widely distributed in my country's Heilongjiang, Liaoning, Gansu, Sichuan, Shandong, In Hubei and other provinces, the growth cycle is one or two generations a year. Tenebrio molitor is rich in nutrients and delicious in taste, and is a protein resource worthy of development and utilization. Its fresh larvae contain about 68.5% Tenebrio protein, 18.9% fat, 1.45% inorganic salt, 0.20% vitamin and 10.95% other. Tenebrio molitor has a complete range of amino acids, and contains 18 essential amino acids and histidine necessary for the human body. The content of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/00
Inventor 吴卫国张凯
Owner CHANGSHA SAIBANG BIOTECH
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